Coexistence of ferromagnetism and superconductivity in YBCO nanoparticles.

Nanoparticles of superconducting YBa(2)Cu(3)O(7-δ) were synthesized via a citrate pyrolysis technique. Room temperature ferromagnetism was revealed in the samples by a vibrating sample magnetometer. Electron spin resonance spectra at selected temperatures indicated that there is a transition from the normal to the superconducting state at temperatures below 100 K.

[Study on the haemostatic efficiency of composite bio-particles].

A porous composite particle (CP) was fabricated by the methods of emulsification and cross-link based on chitosan, alginate and collagen protein, and the tranexamic acid-loaded composite particles (TACP) was prepared by immersing the composite particle into the solution of tranexamic acid and by freeze drying. In the hepatic and splenic hemorrhage model of rabbits, CP and TACP were randomly used as haemostatic agents, and the Suxiaozhixuefen (Flashclot) was used as control. The corresponding hemostatic time and bleeding amount were observed respectively.

Effects of Cu on metabolisms and enzyme activities of microbial communities in the process of composting.

With the compost matrix of pig manure, wheat straw, and spent mushroom substrate, and then inoculated with the Compound Microbe Preparation, the study investigated the effects of the heavy metal Cu on the process of composting. Biolog EcoPlate™ test revealed that at a low content, Cu could improve the capacities of microbial communities to transform and exploit carbon sources in the form of polymer, thus speeding up the decomposition of agricultural wastes, and at a high content, Cu presented inhibiting effect on microbial communities to exploit complex macromolecular carbon sources, thus extending the decomposition of agricultural wastes. Enzyme activity testing showed that at a low content, Cu presented enzyme activity-activating effect at the early period of composting and inhibiting effect in the late period of composting, and at a high content, Cu presented enzyme activity-inhibiting effects through the process of composting.

Molecular genetic features of polyploidization and aneuploidization reveal unique patterns for genome duplication in diploid Malus.

Polyploidization results in genome duplication and is an important step in evolution and speciation. The Malus genome confirmed that this genus was derived through auto-polyploidization, yet the genetic and meiotic mechanisms for polyploidization, particularly for aneuploidization, are unclear in this genus or other woody perennials. In fact the contribution of aneuploidization remains poorly understood throughout Plantae.

Rapamycin prevents endothelial cell migration by inhibiting the endothelial-to-mesenchymal transition and matrix metalloproteinase-2 and -9: an in vitro study.

Purpose: To evaluate the influence of rapamycin on endothelial-mesenchymal transition and matrix metalloproteinase (MMP) secretion by human umbilical vein endothelial cell line EA.hy926 and explore rapamycin's angiogenesis inhibition mechanism.

Methods: EA.

[Surveillance of schistosomiasis in Hubei Province in 2010].

Objective: To understand the endemic situation of schistosomiasis in surveillance sites of Hubei Province in 2010.

Methods: According to The Scheme of Schistosomiasis Surveillance in Hubei Province, 207 endemic villages were selected as surveillance sites, where the surveys of residents, livestock and Oncomelania snails were carried out.

Results: In the 207 surveillance sites, the infection rates of residents and cattle were 1.

Advanced topography-guided (OcuLink) treatment of irregular astigmatism after epikeratophakia in keratoconus with the WaveLight excimer laser.

Purpose: To evaluate the feasibility, safety, and predictability of treatment for high irregular astigmatism with advanced topography-guided laser epithelial keratomileusis in symptomatic eyes after epikeratophakia (EP) for keratoconus.

Methods: In a prospective case series, 10 consecutive eyes (10 patients) with high irregular astigmatism after epikeratophakia for keratoconus received OcuLink laser-assisted subepithelial keratectomy (LASEK) using the Allegretto Wave excimer laser. Uncorrected visual acuity (UCVA), best spectacle-corrected visual acuity (BSCVA), manifestation and refraction, corneal topographic examination with asphericity, and regularity were evaluated.

[Clinical follow-up study of ulnar styloid fractures and classification of distal radial fractures].

Objective: To analyze the result of the distal radial fractures with or without ulnar styloid fracture.

Methods: From January 2007 to October 2010, 62 cases with distal radial fractures were treated by ORIF, of which 52 were followed-up entirely with an average of 15 months (6-34 months), including 6 male cases and 46 female cases at an average age of (62.25±8.

Deep understanding of structure-solubility relationship for a diverse set of organic compounds using matched molecular pairs.

Aqueous solubility is an important biopharmaceutical property in drug discovery and development. Although it has been studied for decades, the impact on solubility by the substructures (or fragments) of compounds are still not fully understood and characterized. This study aims to obtain fragment-solubility relationships using matched molecular pairs, and to provide further insight and suggestions for chemists on structural modifications to improve solubility profiles of drug-like molecules.

Loss of PINK1 function decreases PP2A activity and promotes autophagy in dopaminergic cells and a murine model.

Parkinson's disease (PD) is the most common neurodegenerative movement disorder. Mutations in PTEN-induced kinase 1 (PINK1) are a frequent cause of recessive PD. Autophagy, a pathway for clearance of protein aggregates or impaired organelles, is a newly identified mechanism for PD development.

Tumor Cell Selective Cytotoxicity and Apoptosis Induction by an Herbal Preparation from Brucea javanica.

The plant Brucea javanica has shown impressive efficacy for treating various diseases including cancer. However, the mechanism by which B. javanica acts is poorly understood.

[The preliminary study of establishing animal model of chronic allograft dysfunction after penetrating keratoplasty].

Objective: To establish a murine model of chronic corneal allograft dysfunction (CCAD) after penetrating keratoplasty (PK).

Methods: Experimental study. PK model in mice: Semiallogeneic CB6F1 mice were obtained from matching of female BALB/c and male C57BL/6 mice.

α-Synuclein overexpression impairs mitochondrial function by associating with adenylate translocator.

α-Synuclein (α-syn), a protein involved in the pathogenesis of Parkinson's disease (PD), is known to accumulate in mitochondria, disrupt mitochondrial function. However, the molecular mechanisms that link these pathological responses have not been investigated. In rats overexpressing α-syn in the substantia nigra (SN) through adeno-associated virus (AAV) transduction, about 50% of tyrosine hydroxylase positive neurons were lost after 24 weeks.

Silencing of PINK1 induces mitophagy via mitochondrial permeability transition in dopaminergic MN9D cells.

Accumulation of dysfunctional Mitochondria has been implicated in the pathogenesis of Parkinson's disease (PD). Mutations in PTEN-induced kinase 1 (PINK1), which encodes a putative mitochondrial serine/threonine kinase, have been identified in early-onset forms of PD. Recent data showed that the loss of PINK1 function led to oxidative stress, mitochondrial damage and autophagic elimination of damaged mitochondria.

Clinical features and surgical treatment of peripheral staphyloma.

Purpose: To analyze the anatomical features of peripheral staphyloma and evaluate the clinical effects of double lamellar keratoplasty on this eye disease.

Methods: Four cases of peripheral staphyloma were treated by double lamellar keratoplasty. One third of the degenerative iris was excised, and the iris was returned into the anterior chamber.

Expression of MMP, HPSE, and FAP in stroma promoted corneal neovascularization induced by different etiological factors.

Purpose: To observe the relationship between the expression of matrix metalloproteinases (MMP-2, MMP-9), heparanase (HPSE), and fibroblast activation protein (FAP) in stroma and corneal neovascularization induced by different etiological factors.

Methods: Five models were established: alkaline burn, fungal infection, suturing, immunogen implantation, and tumor cell implantation. The ingrowth time and morphology of corneal neovascularization in each model was observed by slit lamp.

Inhibition of APE1/Ref-1 redox activity with APX3330 blocks retinal angiogenesis in vitro and in vivo.

This study examines the role of APE1/Ref-1 in the retina and its potential as a therapeutic target for inhibiting retinal angiogenesis. APE1/Ref-1 expression was quantified by Western blot. The role of APE1/Ref-1 redox function in endothelial cell in vitro angiogenesis was examined by treating retinal vascular endothelial cells (RVECs) with APX3330, a small molecule inhibitor of APE1/Ref-1 redox activity.

Telomerase recruitment in Saccharomyces cerevisiae is not dependent on Tel1-mediated phosphorylation of Cdc13.

In Saccharomyces cerevisiae, association between the Est1 telomerase subunit and the telomere-binding protein Cdc13 is essential for telomerase to be recruited to its site of action. A current model proposes that Tel1 binding to telomeres marks them for elongation, as the result of phosphorylation of a proposed S/TQ cluster in the telomerase recruitment domain of Cdc13. However, three observations presented here argue against one key aspect of this model.

[Clinical application of irrigation in therapeutic keratoplasty for suppurative keratitis with severe hypopyon].

Objective: To investigate the safety and clinical efficacy of anterior chamber irrigation combined with corneal transplantation in the treatment of corneal ulcer with severe hypopyon.

Methods: Non-randomized retrospective case series. Twenty patients (20 eyes) suffered from corneal ulcer with severe hypopyon were enrolled in Shandong Eye Hospital from January 2008 to June 2009, and 13 patients with significant corneal endothelial plaque, which hard to determine the lesion edge under microscope.

Features of corneal neovascularization and lymphangiogenesis induced by different etiological factors in mice.

Purpose: To analyze the features of corneal neovascularization and lymphangiogenesis induced by different etiological factors.

Methods: A total of 150 mice were randomly divided into five groups to establish models of corneal neovascularization and lymphangiogenesis by alkali burn, suturing, fungal infection, bovine serum albumin (BSA) injection, and tumor cell implantation, respectively. Morphology and length and quantity of corneal neovascularization were monitored daily by slit-lamp microscopy.

Spontaneous closure of macular hole in a patient with x-linked juvenile retinoschisis.

Purpose: To observe macular hole in a patient with juvenile retinoschisis.

Methods And Patient: A 4-year-old boy with X-linked juvenile retinoschisis was examined and followed-up for 2 years. Optical coherence tomography was used to study his maculae.

Establishment of a murine model of chronic corneal allograft dysfunction.

Background: To establish a murine model of chronic corneal allograft dysfunction (CCAD) that permits molecular evaluation of chronic allograft dysfunction after corneal transplantation.

Methods: C57BL/6 (allogeneic), CB6F1 (semiallogeneic) and BALB/c (syngeneic) corneal grafts were transplanted orthotopically to BALB/c recipients and to the BALB/c mice as a control group. The follow-up time was more than 100 days.

Assessment of in silico models for fraction of unbound drug in human liver microsomes.

Importance Of The Field: Fraction of unbound drug in human liver microsome (fu(mic)) incubation media is an important parameter for accurate assessment of hepatic intrinsic clearance and drug-drug interactions. In recent years, there have been considerable advances in understanding structure-microsomal binding relationships.

Areas Covered In The Review: This review highlights the in silico modeling techniques for fu(mic) including physicochemical properties-based modeling, pharmacophore feature-based classification modeling and more complex statistical method-based modeling.

Lack of appreciable species differences in nonspecific microsomal binding.

Species differences in microsomal binding were evaluated for 43 drug molecules in human, monkey, dog and rat liver microsomes, using a fixed concentration of microsomal protein. The dataset included 32 named drugs and 11 proprietary compounds encompassing a broad spectrum of physicochemical properties (11 acids, 24 bases, 8 neutral, c log D -1 to 7, MW 200 to 700 and free fraction <0.001 to 1).