Publications by authors named "Hua'nan Ren"
[Genetic analysis of a case with Al Kaissi syndrome and a literature review].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
February 2024
Objective: To explore the genetic etiology of a child with delayed growth and development and carry out a literature review.
Methods: A child suspected for Al Kaissi syndrome at the First Affiliated Hospital of Zhengzhou University on March 6, 2021 was selected as the study subject. Following extraction of genomic DNA, the child was subjected to copy number variation sequencing (CNV-seq) and whole exome sequencing (WES), and candidate variants were verified by PCR-agarose gel electrophoresis and quantitative real-time PCR (qPCR).
[Prenatal diagnosis of partial deletion of NRXN1 gene with combined CNV-seq and qPCR assays].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
November 2022
Article Synopsis
- The study focused on the genetic diagnosis of 7 fetuses with specific deletions in the NRXN1 gene located at 2p16.3, using low-depth copy number variation sequencing (CNV-seq).
- All 7 fetuses exhibited confirmed deletions through quantitative real-time PCR (qPCR), with details on the size and origin of the deletions documented, including some inherited from parents and some being de novo.
- After genetic counseling, pregnancy outcomes varied: one couple opted for abortion, one case is still ongoing, and five children were born healthy with no reported mental abnormalities.
Article Synopsis
- Trichinellosis is a serious parasitic disease that poses risks to public health and food safety and is caused by the T. spiralis parasite.
- The study focused on metalloproteinase Tsdpy31, which is vital for the molting process of T. spiralis larvae and was shown to be active in various life stages of the parasite.
- Silencing Tsdpy31 reduced larval burden in mice and indicated its importance in cuticle synthesis and embryonic development, suggesting it could be a potential target for vaccine development.
Article Synopsis
- The activation of Trichinella spiralis larvae from muscle larvae (ML) to intestinal infective larvae (IIL) is crucial for infection, with IIL’s excretory/secretory (ES) proteins playing a key role in interacting with the host.
- The study aimed to identify natural active proteases in T. spiralis IIL ES proteins using techniques like Western blot and LC-MS/MS, discovering significant proteolytic activities that assist in tissue invasion.
- A total of 30 proteins were identified, mainly serine proteinases, and the higher expression of certain protease genes at the IIL stage suggests their involvement in the parasite's invasion of the host's intestines and evasion of immune responses.
A Trichinella spiralis aminopeptidase (TsAP) has been identified in intestinal infectious larvae (IIL) and adult worms (AW), but its biological function in the T. spiralis life cycle is unknown. The aim of this study was to characterize TsAP and ascertain its functions in the invasion, development and fecundity of T.
View Article and Find Full Text PDFThe aim of this study was to ascertain the characteristics of a Trichinella spiralis cathepsin X (TsCX) and its role on larval invasion of intestinal epithelial cells (IECs). The full-length of TsCX cDNA sequence was cloned and expressed in Escherichia coli BL21. The results of RT-PCR, IFA and Western blot revealed that TsCX was expressed at T.
View Article and Find Full Text PDFMolting is a key step for body-size expansion and environmental adaptation of parasitic nematodes, and it is extremely important for Trichinella spiralis growth and development, but the molting mechanism is not fully understood. In this work, label-free LC-MS/MS was used to determine the proteome differences between T. spiralis muscle larvae (ML) at the encapsulated stage and intestinal infective larvae (IIL) at the molting stage.
View Article and Find Full Text PDFThe aim of this study was to observe the intestinal mucosal/systemic responses triggered by intranasal vaccination using recombinant Trichinella spiralis serine protease (rTsSP) and its capacity to elicit immune protection against larva challenge in a murine model. rTsSP coupled with cholera toxin B subunit (CTB) was used to vaccinate mice via intranasal route. The results revealed that intranasal vaccination with rTsSP plus CTB elicited significantly intestinal local sIgA response and a TsSP-specific systemic antibody response in vaccinated mice.
View Article and Find Full Text PDFBackground: Trichinella spiralis is an important foodborne zoonotic parasite and it is necessary to develop a vaccine in order to interrupt transmission from animals to humans. A 31 kDa protein from T. spiralis (Ts31) is an antigen targeted by protective antibodies, and Ts31 contains a domain of trypsin-like serine protease that might have the function of serine protease.
View Article and Find Full Text PDFTrichinellosis is one of the most serious foodborne parasitic zoonosis with worldwide distribution, and it is necessary to develop a vaccine to interrupt transmission from animals to humans. Trichinella spiralis adult-specific DNase II-1 (TsDNase II) were identified by immunoproteomics in surface or excretory/secretory proteins of adult worms (AW) and intestinal infective larvae (IIL). The aim of this study was to investigate the systemic, mucosal responses and immune protection elicited by oral vaccination with TsDNase II DNA vaccine delivered by attenuated Salmonella typhimurium strain⊿cyaSL1344.
View Article and Find Full Text PDFBackground: Trichinella spiralis serine protease inhibitor (TsSPI) was identified in ES proteins of adult worms (AW), the TsSPI gene was highly expressed at enteral stage worms (AW and newborn larvae), distributed mainly in the cuticle and stichosome of this nematode. Vaccination of mice with rTsSPI exhibited a 62.2% reduction of intestinal AW and a 57.
View Article and Find Full Text PDFArticle Synopsis
- Identified a protein called TsSP from Trichinella spiralis that may help it invade hosts and grow, with potential as a vaccine target.
- Conducted tests to measure the expression of TsSP during different larval stages and found that it binds to and enters intestinal cells.
- Immunizing mice with TsSP significantly reduced the number of adult worms and larvae, demonstrating TsSP's role in parasite invasion and indicating its potential for vaccine development against Trichinella infections.
The aim of this study was to detect Trichinella spiralis DNA in mouse feces during the early stages of infection using PCR. The target gene fragment, a 1.6kb repetitive sequence of T.
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