2'-Methyl-pyrazolo[4',3':16,17]androst-5-en-3β-ol.

In the title compound, C(21)H(30)N(2)O, there are five fused rings. The A and C rings adopt chair conformations, ring B adopts an 8β,9α-half-chair conformation and ring D adopts a 14α-envelope conformation. The pyrazole ring is planar.

Bioptic significance of incarcerated contents at sclerotomy sites during vitrectomy.

Purpose: To study the clinical significance of incarcerated materials at sclerotomy sites during vitrectomy.

Methods: Fifty samples prolapsed from the entries to standard three-port pars plana vitrectomies were collected along the scleral surface. Samples from rhegmatogenous retinal detachments (RD) (n = 28), traumatic RD (n = 4), miscellaneous vitreous hemorrhages (n = 12), and intraocular foreign bodies (n = 6) were stained with hematoxylineosin and examined under light microscopy after being fixed in paraffin sections or smeared on slides.

[Subretinal transplantation of retinal cells by outer approach].

Objective: To investigate the approaches and effect of pure retinal photoreceptor cells and mixed retinal cells subretinal transplantation on retinal degenerative diseases.

Methods: 16 Kunming mice were divided into two groups: group A and group B (n = 8). Through sclera and choroid, mixed retinal cells were injected into the subretinal space of mice eyes of group A and pure retinal photoreceptors were injected into the subretinal space of mice eyes of group B by using a specific micro-syringe under operative microscopy.

[Effect of endotoxin-induced uveitis on GATA-3 expression and ACAID development].

Objective: To investigate the changes of the GATA-3 expression and development of anterior chamber associated immune deviation (ACAID) after anterior chamber (AC) injection of interphotoreceptor retinoid binding protein (IRBP) under ocular inflammation.

Methods: ACAID was induced by injection of IRBP into the AC of 30 Spar-Dawley (SD) rats. Then the animals were divided into -4 days group, -24 hours group, 0 hour group, 3 days group, and 7 days group according to the lipopolysaccharide (LPS) injection 4 days and 24 hours before, or 0 hour, 3 days and 7 days after IRBP inoculation respectively.

[A study on the effects of rAd-gfp-bcl-X(L) on retinal degeneration in mice].

Objective: To observe the therapeutic effect of rAd-gfp-bcl-X(L) on retinal degeneration (RD) in mice.

Methods: rAd-gfp-bcl-X(L) granules were injected into the subretinal space of right eyes of RD mice in Group A (postnatal days 10, p10) and Group B (postnatal days 22, p22) (n = 20). The morphological changes were observed by light microscopy and transmission electron microscopy at 15 - 30 days post-injection.

[Clinical analysis of tumors of the iris and ciliary body].

Objective: To evaluate the clinical features, different diagnosis, pathological features, management, and prognosis of tumors of the iris and ciliary body.

Methods: Medical records, photographs, pathological findings and the results of follow-up of 37 cases with tumors of the iris and ciliary body were reviewed as a retrospective study.

Results: Of the 37 cases with tumors of the iris and ciliary body, 26 were male and 11 were female.

[Effect of bcl-2 antisense oligonucleotides on multidrug resistance of cultured uveal melanoma cells].

Objective: To evaluate the chemotherapeutic sensitivity of uveal melanoma in vitro and reverse its drug resistance by the delivery of bcl-2 antisense oligodeoxynucleotide (AS-ODN).

Methods: The drug sensitivity tests of primary cultured uveal melanoma cells toward 5-flurouracil (5-FU), thiophosphamide (TSPA), cisplatin (DDP), adriamycin (ADM), vinblastine (VLB), dacarbazine (DTIC) were performed with 3, -4, 5 dimethyliazol-2,5 diphenyl tetrazolium bromide (MTT). AS-ODN bcl-2 were delivered with cationic lipid to down-regulate bcl-2 expression.