A rat model of cerebral small vascular disease induced by ultrasound and protoporphyrin.

Cerebral small vascular disease (CSVD) has a high incidence worldwide, but its pathological mechanisms remain poorly understood due to the lack of proper animal models. The current animal models of CSVD have several limitations such as high mortality rates and large-sized lesions, and thus it is urgent to develop new animal models of CSVD. Ultrasound can activate protoporphyrin to produce reactive oxygen species in a liquid environment.

Accurate nano-injection system for capillary gas chromatography.

The first version of nano-injection device for capillary gas chromatography (cGC) based on inkjet microchip was developed. The nano-injector could accurately control the injection volume in nano-liter, even pico-liter range. Its configuration and mechanism were discussed in detail.

Development of a gel monolithic column polydimethylsiloxane microfluidic device for rapid electrophoresis separation.

A beta-cyclodextrin (beta-CD)-bonded gel monolithic column polydimethylsiloxane (PDMS) microfluidic device was developed in a simple and feasible way. Before preparation of gel monolithic column in PDMS microchannel, PDMS surface was activated by UV light to create silanol groups, which is an active molecule to covalently bond 3-(trimethoxysilyl)-propyl methacrylate (Bind-Silane) and seal microfluidic device. By the way, Bind-Silane is a bifunctional molecule to link polyacrylamide (PAA) gel and inner wall of PDMS microchannel covalently.

Property of ionic liquid in electrophoresis and its application in chiral separation on microchips.

The aqueous solution of a kind of room-temperature ionic liquids (RTILs), 1-ethyl-3-methylimidazolium-tetrafluoroborate (1E-3MI-TFB), demonstrated its exclusive electroosmotic property in microchip electrophoresis. It was applied as the working electrolyte for chiral separation in glass microchip electrophoresis. Compared with boric acid buffer, 1E-3MI-TFB aqueous solution exhibited a broader separation window for enantiomers of dipeptides.

Steady surface modification of polydimethylsiloxane microchannel and its application in simultaneous analysis of homocysteine and glutathione in human serum.

A novel polydimethylsiloxane (PDMS) surface modification method for microchip electrophoresis has been developed to make a stable and sufficient electroosmotic flow (EOF). Poly(l-glutamic acid) (PGA) which had ionizable carboxyl groups at a high pH-range was immobilized on the surface of microchannel fabricated with PDMS. The surface modification involved surface oxidation by plasma, the silanization of 3-aminopropyldimethylethoxysilane (APDMES) and immobilization of PGA via amide bond.

Chiral separation of FITC-labeled amino acids with gel electrochromatography using a polydimethylsiloxane microfluidic device.

A chiral separation model of gel electrochromatography in a polydimethylsiloxane (PDMS) microfluidic device for amino acids (AAs) is presented. Six pairs of fluorescein isothiocyanate (FITC)-labeled dansyl amino acids (Dns-AAs) were separated in a 36-mm effectual separation channel in less than 120 sec, with resolutions all above 0.96.