The Diagnostic Performance of AFP, AFP-L3, DCP, CA199, and Their Combination for Primary Liver Cancer.

Purpose: The prevalence of primary liver cancer (PLC) is rising, yet strategies for its early diagnosis remain inadequate. This study aims to identify novel biomarkers to improve the diagnostic ability of PLC.

Patients And Methods: This study included 94 patients with PLC, 128 patients with benign liver disease (BLD), and 79 normal controls (NC) were included.

OsCBL1 Modulates the Nitrate-Induced Phosphate Response by Altering OsNLP4 Cytoplasmic-Nucleus Shuttling.

Nitrate can directly activate phosphate (Pi) starvation signaling, ultimately promoting plant growth by enhancing phosphorus absorption and utilization and optimizing the balance of nitrogen and phosphorus nutrients. However, the complex mechanisms by which plants integrate complex nutrient signals from nitrogen to phosphorus are not well understood. This study highlights the importance of Calcineurin B-like protein-1 (OsCBL1), a calcium sensor, in coordinating nitrogen and phosphorus signaling in rice.

Study on HIF-PHI combined with iron supplement in treatment of renal anemia in rats.

Background: Roxadustat is a novel hypoxia- inducible factor-prolyl hydroxylase inhibitor(HIF-PHI) used to treat anemia in chronic kidney disease (CKD) patients. It has been reported that roxadustat can slow down kidney damage and delay the development of kidney fibrosis. Anemia and iron deficiency are often associated with the vast majority CKD patients, and insufficient available iron or total iron storage is often the most common cause of anemia and ESAs resistance in CKD patients.

Advances and Challenges in Traumatic Brain Injury from a Forensic Perspective.

Traumatic brain injury (TBI) is one of the leading causes of death and disability. Animal and clinical studies of TBI have greatly changed the clinical practice of TBI with the development and application of new technologies. However, with the development of forensic science, legal issues related to TBI continue to emerge, and it is still far from satisfactory that the practical application of relevant research findings as legal evidence in court practice.

Tensile Properties and Constitutive Model of BFRP-Steel-BFRP Composite Plates.

Traditional materials such as steel and concrete often face limitations in terms of corrosion resistance and long-term performance. Over the past few decades, the search for alternative reinforcement solutions has grown, driven by the need for more sustainable, lightweight, and corrosion-resistant materials. Basalt fibers, with their superior mechanical properties and resistance to environmental degradation, have emerged as a promising candidate.

Recent Studies on the Effects of Static Magnetic Fields (SMF) on Reproductive Function.

Background: With the widespread use of static magnetic fields (SMFs) in applications such as magnetic resonance imaging (MRI) and electric vehicles, concerns have arisen regarding their potential effects on reproductive health. Despite increasing research, the impact of SMFs on reproductive function remains a subject of debate, requiring further exploration.

Methods: This review synthesizes animal and clinical studies on the effects of SMF on reproductive function.

Isoforskolin, adenylate cyclase agonist, inhibits endothelial-to-mesenchymal transition in atherosclerosis.

Background: Atherosclerosis is a common chronic disease characterized by the formation of atheromatous plaques and endothelial dysfunction. Endothelial-to-mesenchymal transition (EndMT) has been identified as a crucial driver of atherosclerosis, with TGF-β serving as a pivotal mediator of EndMT. Isoforskolin (ISOF), derived from the plant Coleus forskohlii, is an effective activator of adenylyl cyclase (AC).

Construction of a circadian rhythm-related gene signature for predicting the prognosis and immune infiltration of breast cancer.

Objectives: In this study, we constructed a model based on circadian rhythm associated genes (CRRGs) to predict prognosis and immune infiltration in patients with breast cancer (BC).

Materials And Methods: By using TCGA and CGDB databases, we conducted a comprehensive analysis of circadian rhythm gene expression and clinicopathological data. Three different machine learning algorithms were used to screen out the characteristic circadian genes associated with BC prognosis.

The Overexpression of RTN4 Significantly Associated With an Unfavourable Prognosis in Patients With Lower-Grade Gliomas.

Gliomas, the most prevalent primary malignancy of the central nervous system, is characterised by its high mortality rates and unfavourable prognosis. Despite extensive research, the underlying mechanisms of glioma pathogenesis remain elusive. The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) databases provided the lower-grade gliomas (LGG) transcriptome and related clinical data, which were downloaded separately.

Manipulation of seed coat content for increasing oil content via modulating BnaMYB52 in Brassica napus.

Seed oil is synthesized in the embryo, which is surrounded by the seed coat. However, the genetic and molecular mechanisms of how seed coat development affects seed oil content (SOC) remains less studied. Through genome-wide association studies in Brassica napus accessions, we identify BnaA09.

Necklace-Structured PVA Sponges for Fast Bleeding Control in the Punctured Femoral Artery Hemorrhage.

Uncontrollable hemorrhage from deep, narrow wounds poses a critical threat to life, as locating the bleeding arteries in such areas is challenging. Rapid bleeding control remains a significant challenge in pre-hospital care. Herein, an injectable hemostatic device filled with disc-like PVA sponges is developed for this objective.

Overexpression of metalloproteinase PAPPA accelerates cancer progression and correlates with immune cell infiltration in gastric cancer: insights from bioinformatics and in vitro investigations.

Background: Gastric cancer (GC) is one of the most common malignant tumors in the digestive system. However, the development of its targeted therapies has been slow. Therefore, exploring the mechanisms of malignant behavior of GC is key to developing their treatment methods.

Feature-Driven Whole-Tissue Imaging with Subcellular Resolution.

Understanding how subcellular structures and events shape tissue-wide phenomena remains a central challenge in biology. We present Multiscale Cleared Tissue Axially Swept Light-Sheet Microscopy (MCT-ASLM), a platform combining cm-scale imaging with targeted high-resolution interrogation of intact tissues in human-guided or autonomous modes. Capable of capturing fields of view up to 21 mm at micron-scale resolution, MCT-ASLM can seamlessly transition to submicron (~300 nm) isotropic resolution for targeted imaging.

The association between dietary creatine intake and cancer in U.S. adults: insights from NHANES 2007-2018.

Background: Creatine has anti-inflammatory, antioxidant, and immunomodulatory effects. However, its impact on tumors remains uncertain.

Methods: This study used data from the National Health and Nutrition Examination Survey (NHANES) from 2007 to 2018 to investigate the relationship between dietary creatine intake and cancer in American adults.

Revolutionizing Tissue Clearing and 3-Dimensional Imaging: Transparent Embedding Solvent System for Uniform High-Resolution Imaging.

Combining transparent embedding with sectioning is likely to be the future direction for tissue clearing and 3-dimensional (3D) imaging. A newly published transparent embedding system, TESOS (Transparent Embedding Solvent System), ensures consistent submicron resolution imaging throughout the entire sample, and can be compatible with different microscopy systems. This method shows great potential in connectome mapping, and might be an optimal option for future 3D multiplex immunofluorescence and RNA in situ hybridization imaging.

Association between cumulative loneliness and risk of cardiovascular diseases in middle-aged and older adults: A longitudinal study.

Objective: Previous longitudinal studies have investigated the association between baseline loneliness and the risk of developing cardiovascular diseases (CVD). Nevertheless, the cumulative effect of loneliness on CVD remains uncertain. The objective of this study is to assess the relationship between the cumulative duration of loneliness and the risk of CVD in middle-aged and older adults.

GSTA2 overexpression alleviates bis (2-ethylhexyl) phthalate (DEHP)-induced male reproductive disorders by inhibiting oxidative stress-mediated cell apoptosis via the activated PI3K/AKT signaling pathway.

Male reproductive disorders are responsible for approximately 50% of infertility cases. Bis (2-ethylhexyl) phthalate (DEHP) is a common environmental pollutant known for its reproductive toxicity. Oxidative stress is a key mechanism in response to DEHP exposure.

Therapeutic Potential of Vanillic Acid in Ulcerative Colitis Through Microbiota and Macrophage Modulation.

This study investigated the protective effects of the dietary polyphenol vanillic acid (VA) on dextran sulfate sodium-induced acute ulcerative colitis (UC) in mice, focusing on its impact on the gut microbiota and inflammatory responses. VA was supplemented following dextran sulfate sodium administration, and key indicators, including body weight, disease activity index, colon length, spleen index, and inflammatory markers, were assessed. VA supplementation significantly alleviated UC symptoms, preserved intestinal barrier integrity, and reduced pro-inflammatory cytokine levels.

Feedback regulation of mA modification creates local auxin maxima essential for rice microsporogenesis.

N-methyladenosine (mA) RNA modification and its effectors control various plant developmental processes, yet whether and how these effectors are transcriptionally controlled to confer functional specificity so far remain elusive. Herein, we show that a rice C2H2 zinc-finger protein, OsZAF, specifically activates the expression of OsFIP37 encoding a core component of the mA methyltransferase complex during microsporogenesis in rice anthers. OsFIP37, in turn, facilitates mA modification and stabilization of an auxin biosynthesis gene OsYUCCA3 to promote auxin biosynthesis in anthers.

Deep imaging of LepR stromal cells in optically cleared murine bone hemisections.

Tissue clearing combined with high-resolution confocal imaging is a cutting-edge approach for dissecting the three-dimensional (3D) architecture of tissues and deciphering cellular spatial interactions under physiological and pathological conditions. Deciphering the spatial interaction of leptin receptor-expressing (LepR) stromal cells with other compartments in the bone marrow is crucial for a deeper understanding of the stem cell niche and the skeletal tissue. In this study, we introduce an optimized protocol for the 3D analysis of skeletal tissues, enabling the visualization of hematopoietic and stromal cells, especially LepR stromal cells, within optically cleared bone hemisections.

Spontaneous bifacial capping of perovskite film for efficient and mechanically stable flexible solar cell.

Flexible perovskite solar cells (F-PSCs) are appealing for their flexibility and high power-to-weight ratios. However, the fragile grain boundaries (GBs) in perovskite films can lead to stress and strain cracks under bending conditions, limiting the performance and stability of F-PSCs. Herein, we show that the perovskite film can facilely achieve in situ bifacial capping via introducing 4-(methoxy)benzylamine hydrobromide (MeOBABr) as the precursor additive.

KDELR3 overexpression as a novel prognostic and diagnostic biomarker in glioma: comprehensive bioinformatic analysis insights.

Glioma, the most prevalent primary intracranial malignancy among adults, is distinguished by its high morbidity and recurrence rates, posing a considerable threat to patients' quality of life and survival prospects. Consequently, the pursuit of efficacious molecular prognostic markers holds paramount importance. The exploration of the role of the KDELR3 kinase family in various neoplastic conditions constitutes an emerging area of research.

Establishment and characterization of a new intestinal-type ampullary carcinoma cell line, DPC-X3.

Ampullary carcinoma (AC) of the intestinal type represents a distinct variant within the broader category of ampullary neoplasms. The scarcity of pertinent cellular models has constrained investigations centered on this particular malignancy. This research effectively generated a cell line (CL) of intestinal-type AC (DPC-X3).