An integrated paper-based microfluidic platform for screening of early-stage Alzheimer's disease by detecting Aβ42.

Alzheimer's disease (AD) is the leading cause of dementia worldwide, and the development of early screening methods can address its significant health and social consequences. In this paper, we present a rotary-valve assisted paper-based immunoassay device (RAPID) for early screening of AD, featuring a highly integrated on-chip rotary micro-valve that enables fully automated and efficient detection of the AD biomarker (amyloid beta 42, Aβ42) in artificial plasma. The microfluidic paper-based analytical device (μPAD) of the RAPID pre-stores the required assay reagents on a μPAD and automatically controls the liquid flow through a single valve.

Multifunctional metal-organic frameworks with photothermal-triggered nitric oxide release for gas/photothermal synergistic cancer therapy.

Photothermal therapy (PTT) utilizing cyanine dyes (Cy) and nitric oxide (NO) gas therapy via BNN6 have demonstrated significant potential in cancer treatment. However, the rapid clearance of these small molecules from the body limits their accumulation at tumor sites, thereby reducing therapeutic efficacy. To address this, we employed the acid-sensitive nanomaterial ZIF-8 as a carrier to encapsulate Cy and BNN6, creating functionalized BNN6-Cy@ZIF-8 Nanoparticles (B-C@Z NPs) for the targeted delivery and release of Cy and BNN6 at tumor sites.

An antimicrobial and adhesive conductive chitosan quaternary ammonium salt hydrogel dressing for combined electrical stimulation and photothermal treatment to promote wound healing.

The aim of this study is to investigate the effect of the adhesive, conductive hydrogel on wound healing when used as a therapeutic dressing. Herein, a dressing of PVA/QCS/TP@Fe (PQTF) was designed and prepared integrating polyvinyl alcohol (PVA), chitosan quaternary ammonium salt (QCS), tea polyphenol (TP), and ferric ions (Fe) by a simple one-pot and freeze-thaw method. In view of the comprehensive properties of PQTF hydrogel, including adhesion, electrical conductivity, and swelling performance, PQTF was selected for subsequent in vitro and in vivo healing promotion studies.

Cauda Equina Syndrome Without Perineal Sensory Changes or Lower Extremity Neurological Deficits Following Postoperative Spinal Epidural Hematoma: A Case Report and Literature Review.

Background: Postoperative spinal epidural hematoma (SEH) is a rare but serious complication following lumbar surgery, with cauda equina syndrome (CES) being one of its most devastating outcomes. While CES typically presents with a combination of bladder and/or bowel dysfunction, diminished sensation in the saddle area, and motor or sensory changes in the lower limbs, atypical cases with isolated urinary symptoms are less recognized and pose significant diagnostic challenges.

Case Presentation: We report the case of a 46-year-old male who developed CES following lumbar microdiscectomy, presenting solely with urinary retention, without the classic signs of lower limb weakness or perineal sensory loss.

[Zero-profile intervertebral fusion with cage-titanium plate for the treatment of multilevel cervical spondylotic myelopathy].

Objective: To explore clinical effect of Zero-profile intervertebral fusion with cage-titanium plate in treating multilevel cervical spondylotic myelopathy.

Methods: From January 2016 to January 2020, 107 patients with multisegmental cervical spondylotic myelopathy treated by surgery were retrospectively analyzed and divided into Hybrid group and control group according to different surgical methods. There were 54 patients in Hybrid group, including 42 males and 12 females, aged from 33 to 77 years old with an average of (57.

Enhancing Chemotherapy Efficacy via an Autologous Erythrocyte-Anchoring Strategy with a Closed-System Drug-Transfer Device.

Chemotherapeutic drugs often fail to localize efficiently to tumors when administered intravenously, causing off-target effects. This study proposes an autologous erythrocyte (ER)-anchoring strategy to improve chemotherapy efficacy and reduce side effects. Utilizing a modified hemodialysis instrument, a closed-system drug-transfer device was developed for autologous ER procurement and immunogenicity mitigation.

Impact of ground-glass component on prognosis in early-stage lung cancer treated with stereotactic body radiotherapy via Helical Tomotherapy.

Purpose: This study aims to investigate the prognostic impact of ground-glass opacity (GGO)-component in early-stage lung cancer patients treated with stereotactic body radiotherapy (SBRT).

Methods: From January 2013 to December 2022, 239 early-stage lung cancer patients (T1-2N0M0) underwent SBRT. They were categorized into two groups based on the presence of GGO-component: 65 patients in the subsolid group with a consolidation tumor ratio (CTR) between 0.

[Prospective study of three-dimensional-printed guide plates assisted hip arthroscopy in treatment of Cam-type femoroacetabular impingement].

Objective: A prospective study was conducted to investigate the feasibility and effectiveness of three-dimensional printed guide plates assisted hip arthroscopy in the treatment of Cam-type femoroacetabular impingement (FAI).

Methods: The clinical data of 25 patients with Cam-type FAI who met the selection criteria between December 2016 and September 2022 were collected. There were 13 males and 12 females with an average age of 42 years (range, 19-66 years).

Effective identification of Alzheimer's disease in mouse models via deep learning and motion analysis.

Spatial disorientation is an early symptom of Alzheimer's disease (AD). Detecting this impairment effectively in animal models can provide valuable insights into the disease and reduce experimental burdens. We have developed a markerless motion analysis system (MMAS) using deep learning techniques for the Morris water maze test.

Start-up of Anammox-HAP in IC reactors: Revelation of sludge characteristics and microbial community structure.

The scarcity of seed sludge poses a significant barrier to the advancement of anaerobic ammonia oxidation (anammox) process. In this investigation, two alternative sludge (anaerobic granular sludge (AGS) and activated flocculent sludge (AFS)) were employed to start up the anammox process in internal circulation (IC) reactors with the hydroxyapatite (HAP) strategy. Both reactors achieved rapid start-up on days 83 and 53, respectively.

Enhancing mesophilic methanogenesis in oleate-rich environments through optimized micro-aeration pretreatment.

Micro-aeration pretreatment has emerged as a promising technology for improving the performance of anaerobic bioreactors in the treatment of lipid-rich organic waste, particularly in mitigating the accumulation of long-chain fatty acids (LCFAs). Micro-aeration intensity is a critical factor in optimizing substrate hydrolysis and methanogenesis efficiency. In this study, optimal micro-aeration intensities for acetoclastic (30 mL-air/g-COD) and overall methanogenesis (7.

Effect of Serum Interleukin-6 Concentration on the Prognosis After Radiotherapy for Patients With Hepatocellular Carcinoma.

The aim of this study was to explore the association between interleukin-6 (IL-6) concentration before radiotherapy (RT) and the prognosis after RT for patients with hepatocellular carcinoma (HCC). The clinical data for 101 patients with HCC who received RT from October 2016 to June 2021 were retrospectively analyzed. In these patients, the tumors were confined to the liver, and IL-6 concentration was measured before RT.

Controllable All-in-One Biomimetic Hollow Nanoscaffold Initiating Pyroptosis-Mediated Antiosteosarcoma Targeted Therapy and Bone Defect Repair.

Pyroptosis has gained attention for its potential to reinvigorate the immune system within the tumor microenvironment. However, current approaches employing pyroptosis inducers suffer from limitations. They primarily rely on single agents, lack precise targeting, and potentially disrupt the intricate bone formation microenvironment, hindering local repair of tumor-induced bone defects.

Perilla Seed Oil: A Review of Health Effects, Encapsulation Strategies and Applications in Food.

Perilla ( L.) is an annual herbaceous plant whose seed oil is rich in unsaturated fatty acids such as alpha-linolenic acid (ALA). This oil exhibits various health benefits, including antioxidant, anti-inflammatory, lipid-lowering, hypoglycemic, neuroprotective and immunomodulatory activities.

Lycium barbarum Extract Enhanced Neuroplasticity and Functional Recovery in 5xFAD Mice via Modulating Microglial Status of the Central Nervous System.

Objective: Alzheimer's disease (AD) is the most prevalent neurodegenerative disease with limited treatment options. This study aimed to investigate the effects of Lycium barbarum extract (LBE), a Chinese herb, on the central nervous system (CNS)-including the retina, brain, and spinal cord-in 5xFAD transgenic mice after the onset of AD.

Methods: Starting at 6 months of age, 5xFAD mice received daily intragastric gavage of LBE (2 g/kg) for 2 months.

Research on the rock cutting performance and feasibility verification of small-scale rotary cutting test for disc cutter.

Small-scale rock cutting tests serve as a simple approach to evaluate the performance of tunnel boring machines (TBMs), but the feasibility of this method requires further investigation. Herein, a small-scale rotary cutting machine is developed to conduct rock cutting tests, and the cutting performance is investigated. The results indicate that similar cutting performance can be achieved through both small-scale and full-scale tests.

Analysis of genotype resistance and HIV-1 transmission risk in HIV-1-infected men who have sex with men in Guiyang, China.

Background: As the social economy has developed and population mobility has increased, differences in the Human immunodeficiency virus type 1 (HIV-1) genotype distribution among men who have sex with men (MSM) have become apparent in the provinces and cities across China. The high variability and drug resistance characteristics of HIV-1 can lead to the widespread spread of resistant strains, which may also result in antiretroviral therapy failure and an increase in the mortality rate.

Objective: The genotypic drug resistance characteristics and HIV-1 transmission risks among HIV-1-infected MSM in Guiyang, Guizhou Province were analyzed in the current study.

A novel method to judge the safe position of C2 lamina screw with double holes.

Background: To evaluate the feasibility of C2 laminar screw (C2LS) fixation assisted by double holes and to explore a novel method for judging its safe positioning.

Methods: The Digital Imaging in Communications format data of 25 C2 vertebrae specimens were obtained by computed tomography thin-slice scanning, and the data were imported into Mimics software for three-dimensional reconstruction. The bone cortex was removed at the starting and terminal midpoints of the lamina as the observation holes.

OsIAA23 Promotes Heading by Directly Downregulating Ghd7 in rice.

Ghd7 is a central regulator to multiple growth and development processes in rice. While it is not clear how Ghd7 is regulated by upstream factors. To identify its upstream regulator, the truncated Ghd7 promoter fragments were used to screen cis elements binding to rice total nuclear proteins.

Photothermally Reinforced Nanozyme Remodeling Tumor Microenvironment of Redox and Metabolic Homeostasis to Enhance Ferroptosis in Tumor Therapy.

The acidity and high GSH level in the tumor microenvironment (TME) greatly limit the antitumor activity of nanozymes. Thus, enhancing nanozymes' activity is fundamentally challenging in tumor therapy. Although the combination of photothermal therapy (PTT) and nanozymes can enhance the catalytic activity, cancer cells will overexpress heat shock proteins (HSPs) at high temperature, aggravating the heat resistance of tumor cells, which in turn compromises the outcome of chemodynamic therapy.