Orthodontic force regulates metalloproteinase-3 promoter in osteoblasts and transgenic mouse models.

Background/purpose: Previously we demonstrated up-regulation of matrix metalloproteinase-3 () in human osteoblasts under compression and in bony specimens of experimental orthodontic tooth movement (OTM). Here, we studied the temporal characteristics of compression stimulation in human and mouse osteoblast cell lines, and generated a transgenic mouse model for assessing the expression during OTM.

Materials And Methods: We investigated expressions in human and murine osteoblasts through RT-PCR and luciferase assay, after compressive force loading.

Development and Evaluation of Multistructured and Hierarchical Epidermal Growth Factor-Poly ( ε -Caprolactone) Scaffolds.

In this paper, we separately fabricated the poly ( ε -caprolactone) (PCL) scaffolds containing epidermal growth factor (EGF) by using our-self fabricated electrospinning machine for tissue regeneration application. Several fundamental properties, including the dimensions, wettability, and EGF release profiles, of the fabricated EGF-PCL bead, fibrous, and multistructured scaffolds were characterized by using the scanning electron microscopy (SEM), contact angle goniometer, and vertical diffusion system. The EGF release profiles of three scaffolds were measured for 200 h, and the multistructured scaffold performed stable and long EGF release properties.

Switchable holographic image splitter fabricated with dye-doped liquid crystals.

Two electrically controllable holographic polarization gratings are spatially multiplexed to serve as an image splitter for further stereogram application. An external AC voltage is used to modulate the diffraction efficiency of the fabricated holographic image splitter for the switching of a 3D stereogram and a 2D plane image. The polarization grating was generated by two writing beams with orthogonal circular-polarization state in dye-doped liquid crystal films.

Fabrication of carbohydrate microarrays through boronate formation.

A straightforward method for fabricating a stable and covalent carbohydrate microarray based on boronate formation between the hydroxyl groups of carbohydrate and boronic acid (BA) on the glass surface was used to identify carbohydrate-protein interactions.