Publications by authors named "Hsu Po Chiang"
Article Synopsis
- Sonothrombolysis, which involves using high mechanical index impulses during an intravenous microbubble infusion, can improve myocardial perfusion in patients with acute ST segment elevation myocardial infarction, but its impact on diastolic dysfunction and left atrial mechanics was assessed for the first time.
- In a study with 100 patients split into two groups (therapy plus PCI vs. PCI only), those receiving sonothrombolysis showed less worsening of diastolic dysfunction and better left atrial mechanics over a follow-up period.
- Overall, the therapy group experienced significantly improved left atrial global longitudinal strain and were less likely to undergo left atrial remodeling compared to the control group, suggesting that sonothrombolysis may offer protective benefits
Background: It has recently been demonstrated that the application of high-energy ultrasound and microbubbles, in a technique known as sonothrombolysis, dissolves intravascular thrombi and increases the angiographic recanalization rate in patients with ST-segment-elevation myocardial infarction (STEMI).
Objective: To evaluate the effects of sonothrombolysis on left ventricular wall motion and myocardial perfusion in patients with STEMI, using real-time myocardial perfusion echocardiography (RTMPE).
Methods: One hundred patients with STEMI were randomized into the following 2 groups: therapy (50 patients treated with sonothrombolysis and primary coronary angioplasty) and control (50 patients treated with primary coronary angioplasty).
Article Synopsis
- Cardiac shock-wave therapy (CSWT) is an emerging treatment for patients with refractory angina, showing immediate and long-term benefits in heart blood flow.
- In a study involving 15 patients who received nine CSWT sessions, treated ischemic heart segments showed a significant increase in myocardial blood flow reserve (MBFR) six months post-treatment.
- Patients also reported improved angina symptoms, with notable decreases in Canadian Cardiovascular Society scores and increases in Seattle Angina Questionnaire scores, all without major cardiovascular events during follow-up.
Article Synopsis
- Preclinical studies indicate that high mechanical index (MI) ultrasound impulses can enhance blood flow during treatment for ST-segment elevation myocardial infarction (STEMI).
- A clinical trial involving 100 patients tested sonothrombolysis combined with standard percutaneous coronary intervention (PCI) against PCI alone to evaluate its effects on heart function and recovery.
- Results showed that patients receiving sonothrombolysis had significantly better outcomes in terms of heart function improvement, reduced infarct size, and higher rates of blood flow restoration compared to those who only underwent PCI.
Non-covalent protein-carbohydrate interactions mediate molecular targeting in many biological processes. Prediction of non-covalent carbohydrate binding sites on protein surfaces not only provides insights into the functions of the query proteins; information on key carbohydrate-binding residues could suggest site-directed mutagenesis experiments, design therapeutics targeting carbohydrate-binding proteins, and provide guidance in engineering protein-carbohydrate interactions. In this work, we show that non-covalent carbohydrate binding sites on protein surfaces can be predicted with relatively high accuracy when the query protein structures are known.
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- * The study uses the antibody-VEGF interaction to demonstrate that amino acid preferences at the antibody-antigen interface can be understood through 3D atomic distributions from protein structure databases, applying machine learning to make predictions.
- * A methodology was developed that can predict amino acid preferences for specific interactions with reasonable accuracy, offering a potential alternative to traditional antibody engineering that relies on animal immune systems.
Article Synopsis
- Protein acetylation, a vital post-translational modification affecting key biological processes, is identified through a new computational method called N-Ace that leverages solvent accessibility and protein properties.
- N-Ace utilizes a two-stage support vector machine to enhance predictive accuracy of acetylation sites by 5% to 14% compared to models that only analyze amino acid sequences.
- Achieving 90% predictive accuracy, N-Ace is user-friendly and serves as a valuable resource for researchers, available for free online.
Article Synopsis
- Protein Post-Translational Modification (PTM) is crucial for regulating various aspects of protein function, such as stability and activity, by altering their physical and chemical properties.* -
- The updated dbPTM2.0 database now serves as an expert system for PTMs, including experimental and predictive data on modification sites along with structural and functional information about proteins.* -
- This new version offers enhanced accessibility to sequence- and structure-based information on PTMs, and is freely available online for researchers and students.*
Tyrosine sulfation is a post-translational modification of many secreted and membrane-bound proteins. It governs protein-protein interactions that are involved in leukocyte adhesion, hemostasis, and chemokine signaling. However, the intrinsic feature of sulfated protein remains elusive and remains to be delineated.
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- Recent studies have focused on protein methylation related to gene transcription, highlighting lysine and arginine as key sites for this modification.
- A new method called MASA combines machine learning with protein sequence and structure data to predict methylation sites on various amino acids, considering solvent-accessible surface area (ASA) for better accuracy.
- Evaluation shows that MASA can predict methylation sites with high accuracy (80.8% for lysine and 85.0% for arginine), and the tool is accessible online for researchers.