Publications by authors named "Hsiu-Lien Herbie Lin"

Currently, glycerol is the most effective cryoprotectant when combined with straw packaging for preserving chicken sperm. Glycerol, however, has toxic effects on sperm cells, which can reduce fertility when present in inseminated semen. Historically, the serial dilution (SD) method was developed to eliminate glycerol and mitigate its adverse effects.

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Glycerol is a cryoprotectant used widely for the cryopreservation of animal sperm, but it is linked to a decrease in fertility. The mechanism underlying the negative effects of glycerol remains unclear. Therefore, in this study, we aimed to gain a better understanding by using the chicken model.

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Article Synopsis
  • A standard concentration of 11% glycerol is used for freezing chicken sperm, but even 2% glycerol can reduce fertility, requiring its removal before artificial insemination.
  • The study developed a simpler glycerol removal method using a sucrose-based Percoll solution, which minimizes osmotic stress on sperm.
  • Results showed that this new method achieved fertility rates similar to conventional techniques while saving 44% of the time and allowing for flexible temperature operation.
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Male subfertility causes are very varied and sometimes related to post-gonadic maturation disruption, involving seminal plasma constituents. Among them, extracellular vesicles are involved in key exchanges with sperm in mammals. However, in birds, the existence of seminal extracellular vesicles is still debated.

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