Amphetamine inhibits the N-methyl-D-aspartate receptor-mediated responses by directly interacting with the receptor/channel complex.

Amphetamine (AMPH) induces behavioral sensitization and neurotoxicity primarily by enhancing the dopamine-mediated neurotransmission. However, the involvement of the N-methyl-D-aspartate (NMDA) receptor in AMPH-induced neuropathology is also known. Recent investigation has found that high concentration of dopamine could inhibit NMDA receptor-mediated responses by blocking the NMDA receptor channel.

Rapid Fluorescence Concentration Immunoassay for Detection of Salmonellae in Chicken Skin Rinse Water.

Salmonellae in chicken carcass rinse water or enrichment broth were detected with a fluorescence concentration immunoassay (FCIA) using intact salmonellae cells as the reacting particles. Sample Solutions and reagents were added to a 96-well fluoricon assay plate and incubated 30 min in the chamber of a fluorescence concentration analyzer at room temperature. The bound and free components were separated by automated vacuum filtration and washings through the membrane filters equipped at the bases of the wells.

A Membrane Blotting Procedure for Detection of Salmonella on Chicken Carcasses.

Salmonellae attached to artificially contaminated chicken carcasses were identified in less than 24 h using a nitrocellulose membrane lift method. Six pieces of nitrocellulose membrane were pressed onto each chicken carcass at the breast, the thigh, and the back in order to lift the salmonellae from the carcasses. After direct incubation of the membranes on xylose lysine tergitol 4 agar overnight at 37°C, the appearance of black colonies on the white membrane was considered a positive presumptive test for salmonellae.