Publications by authors named "Hriju Adhikari"
Wiskott-Aldrich syndrome (WAS) is a rare X-linked disorder characterized by combined immunodeficiency, eczema, microthrombocytopenia, autoimmunity, and lymphoid malignancies. Gene therapy (GT) to modify autologous CD34+ cells is an emerging alternative treatment with advantages over standard allogeneic hematopoietic stem cell transplantation for patients who lack well-matched donors, avoiding graft-versus-host-disease. We report the outcomes of a phase 1/2 clinical trial in which 5 patients with severe WAS underwent GT using a self-inactivating lentiviral vector expressing the human WAS complementary DNA under the control of a 1.
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- The SARS-CoV-2 pandemic originated from animal-to-human spillover, and white-tailed deer have been found to be significantly infected with the virus in North America.
- In a study conducted in Pennsylvania, 16.3% of nasal swab samples from deer tested positive for SARS-CoV-2, revealing the presence of alpha and delta variants in this species.
- The findings indicate that the virus may be circulating among deer populations, suggesting a potential new animal reservoir that could lead to future spillback infections in humans.
Mutations in the genome of SARS-CoV-2 can affect the performance of molecular diagnostic assays. In some cases, such as S-gene target failure, the impact can serve as a unique indicator of a particular SARS-CoV-2 variant and provide a method for rapid detection. Here, we describe partial ORF1ab gene target failure (pOGTF) on the cobas SARS-CoV-2 assays, defined by a ≥2-thermocycle delay in detection of the ORF1ab gene compared to that of the E-gene.
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- Mutations in the SARS-CoV-2 virus can affect how accurately diagnostic tests work, with some mutations, like S gene target failure, serving as indicators of specific variants.
- * The study introduces a new measure called partial ORF1ab gene target failure (pOGTF), which shows high sensitivity (97%) and specificity (99%) for detecting the BA.2.12.1 variant.
- * Monitoring pOGTF rates can quickly track the spread of the BA.2.12.1 variant, making it useful for labs that don’t have sequencing technology, as it correlates with overall test positivity in the community.
β-Thalassemias are inherited anemias that are caused by the absent or insufficient production of the β chain of hemoglobin. Here we report 6-8-year follow-up of four adult patients with transfusion-dependent β-thalassemia who were infused with autologous CD34 cells transduced with the TNS9.3.
View Article and Find Full Text PDFBackground: Mutations in gene regulatory networks often lead to genetic divergence without impacting gene expression or developmental patterning. The rules governing this process of developmental systems drift, including the variable impact of selective constraints on different nodes in a gene regulatory network, remain poorly delineated.
Results: Here we examine developmental systems drift within the cardiopharyngeal gene regulatory networks of two tunicate species, and Cross-species analysis of regulatory elements suggests that -regulatory architecture is largely conserved between these highly divergent species.