: The purpose of this study is to investigate and compare the effects of the antimicrobial agents and bioactive glass nanoparticles activated by femtosecond laser light on the biomimetic dentin remineralization using teeth having carious dentin ICDAS code 3. : A total of 27 dentin surface samples were divided into three groups: the first group was treated with a extract, while the second group was treated with bioactive glass nanoparticles, and as for the control group, the third group received no additional agent. All groups were subjected to femtosecond laser light at three different wavelengths: 390 nm, 445 nm, and 780 nm.
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