Bion/Winnicott encounter: absence, failure and the negative in the work of Bion and Winnicott.

After the death of Freud, a major thrust of the expansion of psychoanalytic theory involved the increasing recognition that the actuality of the emotional functioning of the object,-the primary objects in the infant's development, the analyst as object in the treatment process-were crucial determinants of developmental and therapeutic outcome. This recognition has been the driving force behind the evolution of various iterations of the role of interaction, inter-affectivity and intersubjectivity in two-person theories of psychic development and therapeutic action. This paper attempts to briefly trace in the work of Bion, Winnicott, Green and the Paris Psychosomatic School not only the effects of traumatic occurrences, but of their negative-i.

On the question of the internal frame.

This paper attempts to expand José Bleger's classic, metapsychological descriptions of the psychoanalytic frame to formulate and emphasize the role of the analyst's internal frame in establishing a psychoanalytic observational perspective in the analytic situation. The rationale for doing so follows from clinical necessity, especially when working with patients and psychic organizations that are 'beyond neurosis' and in non-traditional settings such as distance and telemetric analyses. Clinically speaking, in its most effective state, the analyst's internal frame can inform the possibility of an observational vertex aimed at the intuitive grasp of psychic reality rather than a sense-based, empirical observation of parameters denoted by the elements of a consensually validatable social reality.

Simulated galactic cosmic ray exposure activates dose-dependent DNA repair response and down regulates glucosinolate pathways in arabidopsis seedlings.

Outside the protection of Earth's magnetic field, organisms are constantly exposed to space radiation consisting of energetic protons and other heavier charged particles. With the goal of crewed Mars exploration, the production of fresh food during long duration space missions is critical for meeting astronauts' nutritional and psychological needs. However, the biological effects of space radiation on plants have not been sufficiently investigated and characterized.

On looking into 100 years after its publication.

Freud's publication of The Ego and the Id sparked a diverging set of psychoanalytic models - ego psychology, structural conflict theory, Kleinianism, object relations theories, Lacanianism, etc. - each of which attempted to deal with the clinical limitations of his first topography in regard to unconscious guilt, negative therapeutic reactions and primitive character organizations. This paper attempts to look back on these developments from the perspective of contemporary, post-Freudian psychoanalytic theories.

To Feel in My Flesh: Receptivity, Resonance, Representation, and The Beta Screen.

When we are confronted with the challenge of trying to fully convey or describe something about human life and emotional experience, we find ourselves up against the very limitations of language. This problem becomes especially relevant as we attempt to expand psychoanalytic theory so as to enable us to "approach a mental life unmapped by the theories elaborated for the understanding of neurosis" (Bion1962, p. 37).

A Metapsychology of the Unrepresented.

The terms and are increasingly being referred to in psychoanalytic discourse, without our having established a generally agreed upon consensus about their definition, use or meaning. While these particular designations were never used by Freud, a careful reading of his work reveals them to be qualities that characterize the initial state of both the drive and perception. This paper attempts to place these terms in a clinically useful, metapsychological perspective by reviewing their conceptual origin in Freud and examining their elaboration and clinical relevance in the work of Bion, Winnicott, and Green.

Seedlings Display Accession-Specific Morphological and Transcriptomic Responses to the Microgravity Environment of the International Space Station.

Plants have been recognized as key components of bioregenerative life support systems for space exploration, and many experiments have been carried out to evaluate their adaptability to spaceflight. Unfortunately, few of these experiments have involved monocot plants, which constitute most of the crops used on Earth as sources of food, feed, and fiber. To better understand the ability of monocot plants to adapt to spaceflight, we germinated and grew seedlings of the Bd21, Bd21-3, and Gaz8 accessions in a customized growth unit on the International Space Station, along with 1-g ground controls.

Response of and Mizuna Mustard Seeds to Simulated Space Radiation Exposures.

One of the major concerns for long-term exploration missions beyond the Earth's magnetosphere is consequences from exposures to solar particle event (SPE) protons and galactic cosmic rays (GCR). For long-term crewed Lunar and Mars explorations, the production of fresh food in space will provide both nutritional supplements and psychological benefits to the astronauts. However, the effects of space radiation on plants and plant propagules have not been sufficiently investigated and characterized.

NASA's Ground-Based Microgravity Simulation Facility.

Since opportunities to conduct experiments in space are scarce, various microgravity simulators and analogs have been widely used in space biology ground studies. Even though microgravity simulators do not produce all of the biological effects observed in the true microgravity environment, they provide alternative test platforms that are effective, affordable, and readily available to facilitate microgravity research. The Microgravity Simulation Support Facility (MSSF) at the National Aeronautics and Space Administration (NASA) John F.

Trauma, process and representation.

The concept of Trauma in psychoanalysis has suffered from overuse and inconsistent use. A review of Freud's writings beginning with the Project indicates that from the perspective of the impact upon psychic processes, Freud held a more consistent view of the concept that, if recognized, can help avoid the often fruitless etiological debates of internal vs. external cause, intrinsic (drive) vs.

Safety of a New Sinus Irrigation Device in Rhinosinusitis: A Pilot Study.

Background: Puncture and lavage of the paranasal sinuses, previously the primary treatment for unresponsive acute bacterial rhinosinusitis before surgery, has been abandoned due to procedural discomfort and advancements in antibiotic efficacy and endoscopic surgery. The rise in antibiotic-resistant bacteria has renewed the interest in minimally invasive sinus lavage to both avoid aggressive surgical interventions and identify appropriate antibiotic therapy. In this article, we describe the safety and feasibility of a new device in human patients and evaluate its efficacy as a treatment before the traditional sinus surgery in acute rhinosinusitis.

FURTHER THOUGHTS ON TRAUMA, PROCESS AND REPRESENTATION.

Beginning with Freud, throughout his work and in most if not all psychoanalytic formulations, the concept of trauma has been associated with the disruptive effects of excess excitation on psychic regulatory processes and psychic development. Foremost among these are the capacities for emotional containment and representation. The restoration, strengthening or acquisition for the first time of these capacities can take place intersubjectively in a successful analytic therapy and lies at the heart of the therapeutic action.

The compulsion to repeat: An introduction.

The Repetition Compulsion has been the source of much controversy and perplexity. From it's clinical introduction in 1914 in Remembering, Repeating and Working Through to it's metapsychological elaboration in 1920 in Beyond the Pleasure Principle, it has occupied a central position in Freud's thinking. Especially in regard to his later work, it can be seen to be intrinsic to his final dual instinct theory, his theories of the Death Instinct, trauma, memory, binding and action and to the clinical challenges and theoretical changes that led to the formulation of his second topography.

Changes in Nuclear Shape and Gene Expression in Response to Simulated Microgravity Are LINC Complex-Dependent.

Microgravity is known to affect the organization of the cytoskeleton, cell and nuclear morphology and to elicit differential expression of genes associated with the cytoskeleton, focal adhesions and the extracellular matrix. Although the nucleus is mechanically connected to the cytoskeleton through the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex, the role of this group of proteins in these responses to microgravity has yet to be defined. In our study, we used a simulated microgravity device, a 3-D clinostat (Gravite), to investigate whether the LINC complex mediates cellular responses to the simulated microgravity environment.

Hardware Validation of the Advanced Plant Habitat on ISS: Canopy Photosynthesis in Reduced Gravity.

The Advanced Plant Habitat (APH) is the largest research plant growth facility deployed on the International Space Station (ISS). APH is a fully enclosed, closed-loop plant life support system with an environmentally controlled growth chamber designed for conducting both fundamental and applied plant research during experiments extending as long as 135 days. APH was delivered to the ISS in parts aboard two commercial resupply missions: OA-7 in April 2017 and SpaceX-11 in June 2017, and was assembled and installed in the Japanese Experiment Module Kibo in November 2018.

Reflections on Therapeutic Action and the Origins of Psychic Life.

Psychoanalysis has seen a shift in emphasis regarding therapeutic action and technique. A predominant focus on the uncovering or reintegrating of repressed, disguised, or split-off contents has moved to include the intersubjective creation, development, and strengthening of psychic processes and capabilities. The analyst's role in this process has been analogized to that of the primary maternal object in the origins of psychic life.

Rapid Morphological and Cytoskeletal Response to Microgravity in Human Primary Macrophages.

The FLUMIAS (Fluorescence-Microscopic Analyses System for Life-Cell-Imaging in Space) confocal laser spinning disk fluorescence microscope represents a new imaging capability for live cell imaging experiments on suborbital ballistic rocket missions. During the second pioneer mission of this microscope system on the TEXUS-54 suborbital rocket flight, we developed and performed a live imaging experiment with primary human macrophages. We simultaneously imaged four different cellular structures (nucleus, cytoplasm, lysosomes, actin cytoskeleton) by using four different live cell dyes (Nuclear Violet, Calcein, LysoBrite, SiR-actin) and laser wavelengths (405, 488, 561, and 642 nm), and investigated the cellular morphology in microgravity (10 to 10 g) over a period of about six minutes compared to 1 g controls.

Aptamer-enabled uptake of small molecule ligands.

The relative ease of isolating aptamers with high specificity for target molecules suggests that molecular recognition may be common in the folds of natural RNAs. We show here that, when expressed in cells, aptamers can increase the intracellular concentrations of their small molecule ligands. We have named these aptamers as DRAGINs (Drug Binding Aptamers for Growing Intracellular Numbers).

PSYCHOANALYSIS AND THE PROBLEM OF TRUTH.

After briefly reviewing Freud's search for "the truth" in psychoanalytic treatments, the author discusses Bion's views on truth and its prominence in his thinking. The author then addresses various definitions of truth, drawing particularly on recent comments by Ogden (2015). Considerations of the relationship between truth and philosophy, and of that between truth and the arts, follow; the author then returns to a focus on psychoanalytic truth as emergent.

The therapeutic monoclonal antibody market.

Since the commercialization of the first therapeutic monoclonal antibody product in 1986, this class of biopharmaceutical products has grown significantly so that, as of November 10, 2014, forty-seven monoclonal antibody products have been approved in the US or Europe for the treatment of a variety of diseases, and many of these products have also been approved for other global markets. At the current approval rate of ∼ four new products per year, ∼ 70 monoclonal antibody products will be on the market by 2020, and combined world-wide sales will be nearly $125 billion.

A computational study of alternate SELEX.

Systematic evolution of ligands by exponential enrichment (SELEX) is a procedure for identifying nucleic acid (NA) molecules with affinities for specific target species, such as proteins, peptides, or small organic molecules. Here, we extend the work in Seo et al. (Bull Math Biol 72:1623-1665, 2010) (multiple-target SELEX or positive SELEX) and examine an alternate SELEX process with multiple targets by incorporating negative selection into a positive SELEX protocol.