[Expression and significance of hypoxia-inducible factor-1α in lung tissues of obesity-asthma rat].

Objective: To evaluate the expression level of hypoxia-inducible factor-1α (HIF-1α) in the rat model of diet-induced obesity and asthma.

Methods: Forty male specific pathogen-free SD rats were randomly divided into four groups: normal body mass control group (group A), asthmatic rats with normal body mass (group B), obese control group (group C) and obese asthmatic rats (group D). The rats in both group A and B were fed basic diet, while those in group C and D were fed high-fat diet in order to establish diet-induced obese rat model.

[Expression and significance of hypoxia-inducible factor-1α in patients with chronic obstructive pulmonary disease and smokers with normal lung function].

Objective: To study the expression of hypoxia-inducible factor (HIF)-1α in lung tissues and sera from chronic obstructive pulmonary disease (COPD) patients and smokers with normal lung function and explore its clinical significance.

Methods: Lung tissue samples were obtained from 32 patients undergoing surgery for peripheral lung tumors. Lung function test was performed before lung cancer surgery.

[Effect of leptin on lung fibrosis of silicosis rats and the correlation between leptin and HIF-1α expressions].

Objective: To observe the expression of leptin (LP) and its influence on pulmonary fibrosis in experimental rats with silicosis and the correlation between the expressions of leptin and HIF-1α.

Methods: A total of 120 male Sprague-Dawley rats were randomly divided into normal control group, silicosis model group, LP intervention groups (consisting of LP5, LP10 and LP20 groups according to the concentration of LP). The rats in the normal control group were intratracheally administered 1 mL normal sodium, and the ones in the other groups were intratracheally given 1 mL SiO₂(40 mg/mL) suspension.

[Expression and significance of immunoglobulin free light chains in serum and lung tissues from patients with chronic obstructive pulmonary disease].

Objective: To study the association of free immunoglobulin light chain (FLC) with clinical manifestations and lung inflammation in smokers with normal lung function and chronic obstructive pulmonary disease (COPD) patients.

Methods: Thirty-two patients with peripheral lung cancer undergoing surgical resection were enrolled from the Department of Thoracic Surgery,Affiliated Hospital of Xuzhou Medical College. They were divided into non-smoking with normal lung function group (non-smoking group, 10 cases), smoking with normal lung function group (smoking group, 12 cases) and smoking with stable COPD group (COPD group, 10 cases).

[The effect of moxifloxacin on apoptosis of airway smooth muscle cells and mitochondria membrane potential].

Objective: To observe the effects of moxifloxacin at various concentrations on the expression of Caspase-3, the alteration of mitochondria membrane potential (ΔΨm) and the apoptosis of airway smooth muscle cells (ASMCs), and to explore the possible mechanisms.

Methods: ASMCs were derived from rat airway tissues and cultured in vitro. The cells were randomly divided into 5 groups including a control group and 4 groups to which moxifloxacin was added at different concentrations (40, 80, 120, 200 mg/L, groups M40, M80, M120 and M200 respectively).