Expression Analysis of Oxidative Stress Markers 8-hydroxydeoxyguanosine and Protein Carbonyl in Breast Cancer and Their associations with Certain Immunological and Tumor Markers.

Objective: Breast cancer (BC) is a heterogeneous disease, recognized as a major public health problem worldwide, with several risk factors including oxidative stress, an imbalance between the production of reactive oxygen species (ROS) and antioxidant capacity. The aim of this study is to evaluate oxidative stress markers 8-Hydroxydeoxyguanosine (8-OHdG) and protein carbonyl and their associations with pathological and immunohistochemical parameters as well as the immune system in particular Interleukin-6 (IL-6) in BC patients from western Algeria.

Methods: We analyzed serum concentrations of 8-OHdG and IL-6 by Eliza technique and protein carbonyl by derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH), then associated them with immunohistochemical and pathological parameters, as well as in the different molecular and histological groups of BC.

Expression profile of interleukin-6, 4-hydroxy-2-nonenal, and hypoxia-inducible factor 1-α in women with breast cancer and their association with clinicopathological parameters.

Introduction: Breast cancer (BC) is the most common malignancy in women worldwide, representing a major public health burden. Therefore, there is a need to identify circulating biomarkers for the early detection of BC and to facilitate the diagnosis. Interleukin-6 (IL-6), 4-hydroxy-2-nonenal (4-HNE), and hypoxia-inducible factor 1-α (HIF-1α) are biomarkers involved in the initiation and progression of this disease.