Selection of Specific Aptamer against Rivaroxaban and Utilization for Label-Free Electrochemical Aptasensing Using Gold Nanoparticles: First Announcement and Application for Clinical Sample Analysis.

For the first time, a novel aptamer was designed and utilized for the selective detection of rivaroxaban (RIV) using the integration of bioinformatics with biosensing technology. The selected aptamer with the sequence 5'-TAG GGA AGA GAA GGA CAT ATG ATG ACT CAC AAC TGG ACG AAC GTA CTT ATC CCC CCC AAT CAC TAG TGA ATT-3' displayed a high binding affinity to RIV and had an efficient ability to discriminate RIV from similar molecular structures. A novel label-free electrochemical aptasensor was designed and fabricated through the conjugation of a thiolated aptamer with Au nanoparticles (Au-NPs).

Quantification of methotrexate in plasma samples using highly fluorescent nanoparticles.

A novel amine-functionalized silica carbon polymer dots (APTES-CPDs)-based sensor was developed for methotrexate (MTX) detection. APTES-CPDs core-shell structure was synthesized via a one-step hydrothermal process and were employed as an optical sensor for MTX determination due to quenching effect of MTX on the fluorescence intensity of CPDs. Under optimum conditions, the developed sensor detects MTX in plasma samples in the dual linear ranges of 10 ng/mL to 2.

Correction: Determination of aflatoxin M1 using an aptamer-based biosensor immobilized on the surface of dendritic fibrous nano-silica functionalized by amine groups.

Correction for 'Determination of aflatoxin M1 using an aptamer-based biosensor immobilized on the surface of dendritic fibrous nano-silica functionalized by amine groups' by Houman Kholafazad kordasht , , 2019, , 3910-3919, DOI: 10.1039/C9AY01185D.

An innovative electrically conductive biopolymer based on poly(β-cyclodextrin) towards recognition of ascorbic acid in real sample: Utilization of biocompatible advanced materials in biomedical analysis.

In this study, a sensitive platform was designed for the electrocatalytical oxidation and recognition of ascorbic acid (AA) based on poly(β-cyclodextrin) modified glassy carbon electrode (p(β-CD-GCE). Electropolymerization of β-CD on the surface of GCE was performed on the potential range of -1 to 1.5 V.

A flexible paper based electrochemical portable biosensor towards recognition of ractopamine as animal feed additive: Low cost diagnostic tool towards food analysis using aptasensor technology.

Due to the costly and time-consuming traditional techniques, providing a low-cost, portability and flexibility diagnostic tool with the ability to monitor and detect various animal feed additive is highly demanded. Over the years, paper-based biosensors have emerged as point of care (POC) diagnostic, easy-to-use and miniaturized tools. However, they have been suffered from low sensitivity.

Electrochemical immunoplatform to assist in the diagnosis of oral cancer through the determination of CYFRA 21.1 biomarker in human saliva samples: Preparation of a novel portable biosensor toward non-invasive diagnosis of oral cancer.

In this study, a novel, low-cost, and flexible paper-based electrochemical immunosensor was developed for the bioanalysis of Cyfra 21.1 biomarker in human saliva samples by using stabilization of synthesis Ag nano-ink on the surface of paper using pen-on-paper technology. The employed electrochemical techniques for the evaluation of immunoplatform performance were differential pulse voltammetry and chronoamperometry.

Trifluralin recognition using touch-based fingertip: Application of wearable glove-based sensor toward environmental pollution and human health control.

Monitoring of herbicides and pesticides in water, food, and the environment is essential for human health, and this requires low-cost, portable devices for widespread deployment of this technology. For the first time, a wearable glove-based electrochemical sensor based on conductive Ag nano-ink was developed for the on-site monitoring of trifluralin residue on the surface of various substrates. Three electrode system with optimal thicknesses was designed directly on the finger surface of a rubber glove.

Sensitive aptasensing of ciprofloxacin residues in raw milk samples using reduced graphene oxide and nanogold-functionalized poly(amidoamine) dendrimer: An innovative apta-platform towards electroanalysis of antibiotics.

The constant need of humans and animals for food resources was led to overuse of antibiotics as vital medicines. In this regard, we are now facing major concern about the risks on the food safety and environment owing to their uncontrolled disposal. Hence, the progress of simple and sensitive approaches for fast monitoring of antibiotic levels is highly desirable.

A stretchable glove sensor toward rapid monitoring of trifluralin: A new platform for the on-site recognition of herbicides based on wearable flexible sensor technology using lab-on-glove.

In this study, a flexible glove-based electrochemical sensor as a wearable point-of-use screening tool has been fabricated for defense and food security applications. To design the wearable glove-based sensor, we drew conductive patterns on the fingers of a rubber glove via gold@silver-modified graphene quantum dots (Au@Ag core-shell/graphene quantum dots [GQDs]) nano-ink with optimal thickness. Then, this platform is combined with a portable electrochemical analyzer for on-site detection of trifluralin pesticide in the range of 10 nM to 1 mM with the low limit of quantification (LLOQ) of 10 nM.

Biomedical analysis of exosomes using biosensing methods: recent progress.

Exosomes are membrane-bound extracellular vesicles (EVs) that are produced in the endosomal compartments of most eukaryotic cells; they play important roles in intercellular communication in diverse cellular processes and transmit different types of biomolecules. Endocytic pathways release exosomes, which have diameters ranging from 50 to 200 nm. The unique functions of exosomes have been introduced as cancer bio-markers due to the cargo (protein, DNA and RNA) of external exosomes (tetraspanin) and internal exosomes (syntenin).

Aptamer based recognition of cancer cells: Recent progress and challenges in bioanalysis.

Rapid and accurate monitoring of cancer cells with high sensitivity is essential for a successful cancer treatment. As high-affinity nucleic acid ligands, aptamers can improve the properties of detection methods by conjugating with intracellular or extracellular cancer biomarkers. Despite the advances in the early detection and treatment of cancer cells, lacking effective early detection tools is one of the causes of a high mortality rate.

Biosensing of microcystins in water samples; recent advances.

Safety and quality of water are significant matters for agriculture, animals and human health. Microcystins, as secondary metabolite of cyanobacteria (blue-green algae) and cyclic heptapeptide cyanotoxin, are one of the main marine toxins in continental aquatic ecosystems. More than 100 microcystins have been identified, of which MC-LR is the most important type due to its high toxicity and common detection in the environment.

Specific monitoring of aflatoxin M1 in real samples using aptamer binding to DNFS based on turn-on method: A novel biosensor.

In the present work, a novel biocompatible scaffold was fabricated for the DNA aptamer immobilization. For the first time, amino-functionalized dendritic fibrous nanosilica (KCC-1-nPr-NH ) and gold nanoparticle supported by chitosan (AuNPs-CS) were synthesized and electrodeposited successfully on the surface of the glassy carbon electrode by chronoamperometry technique. Unique oligonucleotide of aflatoxin M1 (5'-ATC CGT CAC ACC TGC TCT GAC GCT GGG GTC GAC CCG GAG AAA TGC ATT CCC CTG TGG TGT TGG CTC CCG TAT) labeled by toluidine blue was immobilization on the prepared interface.

Determination of aflatoxin M1 using an aptamer-based biosensor immobilized on the surface of dendritic fibrous nano-silica functionalized by amine groups.

Aflatoxins are potential food pollutants produced by fungi. Among them, aflatoxin M1 (AF M1) is the most toxic. A great deal of concern is associated with AF M1 toxicity.