[The design and use of a mobile intensive care unit ambulance].

Objective: To improve the range of use and efficiency of first-aid in a disaster, a mobile intensive care unit (ICU) ambulance was designed and evaluated.

Methods: The data of disasters, accidental injury in astronauts, injuries occurred in conflicts in foreign nations were reviewed and analyzed, together with our clinical experience of first-aid, a mobile ICU in an ambulance was designed.

Results: Provided with automatic cardiopulmonary resuscitation apparatus and instruments for surgical life-saving operation, the mobile ICU ambulance could allow the occupants to perform effective rescue means during rush hour of urban traffic.

[Investigation of fucoidanase by FTIR spectra].

Fucoidanase was isolated and purified from marine fungus LD8 by solid state fermentation, extraction with citric acid buffer, acetone precipitatation and column chromatography on Sephadex G-100. A single band on PAGE shows that pretty pure fucoidanase has been obtained. FT-IR spectra and its derivation, self-deconvolution and curve-fitting methods were used to analyze the secondary structure of the fucoidanase.

[Study on the interaction of cystine and polyphenol oxidase from nicotian tobaccum].

The interaction of cystine and polyphenol oxidase (PPO) from nicotian tobaccum has been studied. The results show that cystine has an inhibitory effect on the enzymatic activity, the mechanism of which might be that the thiolether in cystine coordinates with Cu2+ in the active site of PPO, and the inactivation constant value of PPO by cystine is 0.633 min(-1), while the substrates or the products inhibit their combination.

[Fluorescence study on the interaction of salicylic acid and bovine serum albumin].

The interaction between salicylic acid and bovine serum albumin has been studied by fluorescence spectroscopy. The results show that the quenching mechanism of the combination of bovine serum albumin with salicylic acid is a static quenching procedure, the quenching constant K(sv) is 1.097 x 10(4) (mol x L(-1))(-1), and the equilibrium constant is 7.