Yttrium vanadates based ratiometric fluorescence probe for alkaline phosphatase activity sensing.

Alkaline phosphatase (ALP) is an important biomarker for diagnosis, and the abnormal level of serum ALP is closely related to a variety of diseases. In present work, a ratiometric fluorescence probe based on hybrid nanoparticles CDs@YVO: Dy nanoparticle is introduced for alkaline phosphatase (ALP) activity determination. The CDs@YVO: Dy probe is constructed by the carbon dots (CDs) and YVO: Dy through a simple mixing method, in which the blue emission of CDs at 405 nm acts as the calibrated signal, the green emission of YVO: Dy at 574 nm decreased with the increasing targets ALP, and used as the output signal.