Optimized automated radiosynthesis of F-JNJ64413739 for purinergic ion channel receptor 7 (P2X7R) imaging in osteoporotic model rats.

Objective: To optimize the automated radiosynthesis of the purinergic ion channel receptor 7 (P2X7R) imaging agent F-JNJ64413739 and evaluate its potential for brain imaging in osteoporotic model rats.

Methods: A more electron-deficient nitropyridine was employed as the labeling precursor to facilitate the F-labeling. The radiosynthesis was conducted on an AllinOne synthesis module, and followed by purification via high-performance liquid chromatography (HPLC).

Pimpinellin mitigates DSS-induced ulcerative colitis in mice by reducing inflammation and regulating gut microbiota.

Background: Pimpinellin is a phenylpropanoid compound involved in various physiological and pathological processes in the body. The specific impact of pimpinellin on ulcerative colitis (UC) remains uncertain. Research on pimpinellin by using a dextran sulfate sodium (DSS)-induced UC model can lay the groundwork for future drug screening and various treatment approaches for UC.

Comprehensive analysis of Capsicum annuum CaLhcs uncovered the roles of CaLhca5.1 and CaLhcb1.7 in photosynthesis and stress tolerance.

The light-harvesting chlorophyll a/b-binding proteins (Lhcs) are integral to plants' capture and transfer of light energy during photosynthesis. However, the Lhc gene family remains unexplored in pepper. In this study, 37 CaLhcs (Capsicum annuum Lhc) were identified from the reference genome and classified into five subfamilies (Lhca, Lhcb, CP24, CP26, and CP29) based on phylogenetic relationships and conserved domains, with members of each subfamily displaying similar conserved motifs and gene structures.

Synthesis, preclinical assessment, and first-in-human study of [F]d-FET for brain tumor imaging.

Purpose: Tumor-to-background ratio (TBR) is a critical metric in oncologic PET imaging. This study aims to enhance the TBR of [F]FET in brain tumor imaging by substituting deuterium ("D") for hydrogen ("H"), thereby improving the diagnostic sensitivity and accuracy.

Methods: [F]d-FET was synthesised by two automated radiochemistry modules.

Establishment of recombinase polymerase amplification detection method for Dactylobotrys graminicola.

Hulless barley sheath rot is a spike disease caused by Dactylobotrys graminicola. In recent years, it has generally occurred in hulless barley growing areas in China, resulting in reduced hulless barley yields. In this study, primers and probes were designed based on conserved genome sequences, and a method was established using recombinant enzyme polymerase amplification-lateral flow burette (RPA-LFD) technology for the rapid diagnosis of sheath rot in hulless barley.

A nickel porphyrin-based covalent organic framework modified electrode for the electrochemical detection of acetaminophen.

Covalent organic frameworks (COFs) can be rationally designed with functional organic ligands to improve the electrochemical responsiveness of the electrode toward certain medicinal compounds. In this study, we synthesized a COF-Ni electrocatalyst material, which is formed by covalent coupling of electron-rich 2,3,6,7-tetrakis (4-formylphenyl) tetrakis (4-imidazolyl) (TTF-4CHO) and hole-rich 5,10,15,20-tetrakis (4-aminophenyl) porphyrin nickel(II) (TAPP-Ni). The reasonable electron transfer path design, the large specific surface area of the COF and the physical properties of ordered nanopores, as well as the Ni-N bond as a highly active catalytic center, allow the COF-Ni material modified electrode to exhibit excellent sensing performance for acetaminophen (ACOP).

Synergetic Enzyme-Incorporated Metal-Organic Framework and Polyoxometalate Nanozyme: Achieving Stable Tandem Catalysis for Organic Photoelectrochemical Transistor Bioanalysis.

Organic photoelectrochemical transistor (OPECT) has emerged as a promising technique for biomolecule detection, yet its operational rationale remains limited due to its short development time. This study introduces a stable tandem catalysis protocol by synergizing the enzyme-incorporated metal-organic frameworks (E-MOFs) with polyoxometalate (POM) nanozyme for sensitive OPECT bioanalysis. The zeolitic imidazolate framework-8 (ZIF-8) acts as the skeleton to protect the encapsulated glucose oxidase (GOx), allowing the stable catalytic generation of HO.

Seasonal succession of endophyte and the association with active ingredients in .

Unlabelled: The endophyte is closely related to medicinal plant growth and development, stress resistance, and active ingredients' accumulation. However, a seasonal succession of endophytes and the association with active ingredients is still unclear. In this study, we used high-throughput sequencing methods to compare the endophyte diversity of under different seasons and analyze the association between endophytes and five active ingredients.

Preclinical characterization of [F]D-LW223: an improved metabolically stable PET tracer for imaging the translocator protein 18 kDa (TSPO) in neuroinflammatory rodent models and non-human primates.

Positron emission tomography (PET) targeting translocator protein 18 kDa (TSPO) can be used for the noninvasive detection of neuroinflammation. Improved in vivo stability of a TSPO tracer is beneficial for minimizing the potential confounding effects of radiometabolites. Deuteration represents an important strategy for improving the pharmacokinetics and stability of existing drug molecules in the plasma.

Cascading CRISPR/Cas and Nanozyme for Enhanced Organic Photoelectrochemical Transistor Detection with Triple Signal Amplification.

Innovative signal amplification and transduction play pivotal roles in bioanalysis. Herein, cascading CRISPR/Cas and the nanozyme are integrated with electronic amplification in an organic photoelectrochemical transistor (OPECT) to enable triple signal amplification, which is exemplified by the miRNA-triggered CRISPR/Cas13a system and polyoxometalate nanozyme for OPECT detection of miRNA-21. The CRISPR/Cas13a-enabled release of glucose oxidase could synergize with peroxidase-like SiW to induce catalytic precipitation on the photogate, inhibiting the interfacial mass transfer and thus the significant suppression of the channel current.

Multiscale Tunable Nanorings Based on Bi-Component Micellar-Configuration-Transformation Induced by Hydrophobicity.

Ringy nanostructures are amazing materials, displaying unique optical, magnetic, and electronic properties highly related to their dimensions. A strategy capable of continuously tailoring the diameter of nanorings is the key to elucidating their structure-function relationship. Herein, a method of bi-component micellar-configuration-transformation induced by hydrophobicity for the synthesis of nanorings with diameters ranging from submicron (≈143 nm) to micron (≈4.

The sparing effect of ultra-high dose rate irradiation on the esophagus.

Background And Purpose: Current studies have substantiated the sparing effect of ultra-high dose rate irradiation (FLASH) in various organs including the brain, lungs, and intestines. Whether this sparing effect extends to esophageal tissue remains unexplored. This study aims to compare the different responses of esophageal tissue in histological and protein expression levels following conventional dose rate irradiation (CONV) and FLASH irradiation to ascertain the presence of a sparing effect.

Optical Surface-guided Radiation Therapy for Upper and Lower Limb Sarcomas: An Analysis of Setup Errors and Clinical Target Volume-To-Planning Target Volume Margins.

Purpose: To assess the clinical benefits of surface-guided radiation therapy (SGRT) in terms of setup error, positioning time, and clinical target volume-to-planning target volume (CTV-PTV) margin in extremity soft tissue sarcoma (STS).

Methods And Materials: Fifty consecutive patients treated with radiation therapy were selected retrospectively. Treatment setup was performed with either laser-based imaging only (control group), or with laser-based and daily optical surface-based imaging (SGRT group).

Alterations of apparent diffusion coefficient from ultra high b-values in the bilateral thalamus and striatum in MRI-negative drug-resistant epilepsy.

Objective: Subcortical nuclei such as the thalamus and striatum have been shown to be related to seizure modulation and termination, especially in drug-resistant epilepsy. Enhance diffusion-weighted imaging (eDWI) technique and tri-component model have been used in previous studies to calculate apparent diffusion coefficient from ultra high b-values (ADCuh). This study aimed to explore the alterations of ADCuh in the bilateral thalamus and striatum in MRI-negative drug-resistant epilepsy.

Parameter identifiability of a within-host SARS-CoV-2 epidemic model.

Parameter identification involves the estimation of undisclosed parameters within a system based on observed data and mathematical models. In this investigation, we employ DAISY to meticulously examine the structural identifiability of parameters of a within-host SARS-CoV-2 epidemic model, taking into account an array of observable datasets. Furthermore, Monte Carlo simulations are performed to offer a comprehensive practical analysis of model parameters.

A nomogram based on conventional and contrast-enhanced ultrasound radiomics for the noninvasively prediction of axillary lymph node metastasis in breast cancer patients.

Background: This study aimed to investigate whether quantitative radiomics features extracted from conventional ultrasound (CUS) and contrast-enhanced ultrasound (CEUS) of primary breast lesions can help noninvasively predict axillary lymph nodes metastasis (ALNM) in breast cancer patients.

Method: A total of 111 breast cancer patients with 111 breast lesions were prospectively enrolled. All the included patients received presurgical CUS screening and CEUS examination and were randomly assigned to the training and validation sets at a ratio of 7:3 (n = 78 versus 33).

Alleles on locus chromosome 4B from different parents confer tiller number and the yield-associated traits in wheat.

Pleiotropy is frequently detected in agronomic traits of wheat (Triticum aestivum). A locus on chromosome 4B, QTn/Ptn/Sl/Sns/Al/Tgw/Gl/Gw.caas-4B, proved to show pleiotropic effects on tiller, spike, and grain traits using a recombinant inbred line (RIL) population of Qingxinmai × 041133.

A deep learning-based method for the prediction of temporal lobe injury in patients with nasopharyngeal carcinoma.

Purpose: To establish a deep learning-based model to predict radiotherapy-induced temporal lobe injury (TLI).

Materials And Methods: Spatial features of dose distribution within the temporal lobe were extracted using both the three-dimensional convolution (C3D) network and the dosiomics method. The Minimal Redundancy-Maximal-Relevance (mRMR) method was employed to rank the extracted features and select the most relevant ones.

Two-Hour Nicotine Withdrawal Improves Inhibitory Control Dysfunction in Male Smokers: Evidence from a Smoking-Cued Go/No-Go Task ERP Study.

Purpose: Nicotine withdrawal is a multifaceted physiological and psychological process that can induce a spectrum of mood disturbances. Gaining a more nuanced understanding of how pure nicotine withdrawal influences cognitive control functions may provide valuable insights for the enhancement of smoking cessation programs. This study investigated changes in inhibitory control function in smokers after 2-hour nicotine withdrawal using the event-related potential (ERP) technique.

Highly Efficient Thiolate-Based Ionic Liquid Catalysts for Reduction of CO: Selective N-Functionalization of Amines to Form N-Formamides and N-Methylamines.

Developing efficient catalysts to convert CO into value-added chemicals is valuable for reducing carbon emissions. Herein, a kind of novel thiolate-based ionic liquid with sulfur as the active site was designed and synthesized, which served as highly efficient catalyst for the reductive N-functionalization of CO by amines and hydrosilane. By adjusting the CO pressure, various N-formamides and N-methylamines were selectively obtained in high yields.