Whole-Genome Sequencing for Tracing the Genetic Diversity of and Isolated from Livestock in Egypt.

Brucellosis is a highly contagious zoonosis that occurs worldwide. Whole-genome sequencing (WGS) has become a widely accepted molecular typing method for outbreak tracing and genomic epidemiology of brucellosis. Twenty-nine spp.

Reduction of thrombogenicity of PVC-based sodium selective membrane electrodes using heparin-modified chitosan.

Heparin-modified chitosan (H-chitosan) membrane was utilized to enhance biocompatibility of sodium selective membrane electrode based on the highly thrombogenic polyvinyl chloride (PVC). Sodium ion sensing film was prepared using PVC, sodium ionophore-X, potassium tetrakis(chlorophenyl)-borate, and o-nitrophenyloctylether. The PVC-based sensing film was sandwiched to chitosan or H-chitosan to prevent platelet adhesion on the surface of PVC.

Flow injection spectrofluorimetric determination of iron in industrial effluents based on fluorescence quenching of 1-naphthol-2- sulfonate.

A sensitive and selective spectrofluorimetric method has been developed for flow injection analysis (FIA) of iron(III) based on its fluorescence quenching effect on the water soluble 1-naphthol-2-sulfonate. The fluorescence emission spectra were collected with excitation at 283 nm. The emission peaks of the neutral and anionic forms of 1-naphthol-2-sulfonate as well as the band area were found to decrease linearly with iron(III) concentrations over the range 0.

A simple-potentiometric method for determination of acid and alkaline phosphatase enzymes in biological fluids and dairy products using a nitrophenylphosphate plastic membrane sensor.

A novel poly(vinyl chloride) matrix membrane sensor responsive to 4-nitrophenylphosphate (4-NPP) substrate is described, characterized and used for the potentiometric assay of acid (ACP) and alkaline (ALP) phosphatase enzymes. The sensor is based on the use of the ion-association complex of 4-NPP anion with nickel(II)-bathophenanthroline cation as an electroactive material and nitrophenyloctyl ether (NPOE) as a solvent mediator. The sensor displays good selectivity and stability and demonstrates a near-Nernstian response for 4-NPP over the concentration range 9.

Selective potentiometric determination of nitrite ion using a novel (4-sulphophenylazo-)1-naphthylamine membrane sensor.

A novel polymeric membrane sensor sensitive to (4-sulphophenylazo-)1-naphthylamine (SPAN) based on the use of tris(bathophenanthroline) Ni(II)-SPAN ion pair as an ion exchanger in plasticised PVC membrane is described. The sensor exhibits a linear calibration plot with near-Nernstian anionic slope of -55.0+/-0.