Immunocytochemical study of proteinase F in the mouse submandibular gland.

Esteroproteinases extracted from the submandibular gland (SMG) of normal male mice were fractionated by isoelectric focusing into three major peaks with isoelectric point (pI) values of 9.9 (P-esterase), 5.8 (proteinase A), and 5.

Inhibitory effect of androgen on the synthesis of proteinase F in the male mouse submandibular gland.

Androgenic regulation of one of the esteroproteinases (proteinase F) in the mouse submandibular gland was studied using specific antiserum. In contrast to esteroproteinases such as proteinases A, D or P-esterase, proteinase F content in male but not in female mice was increased by gonadectomy and decreased by the injection of various androgens. In-vivo incorporation of [3H]leucine into proteinase F in males was increased after castration and decreased by the injection of testosterone propionate; androgens inhibited the de-novo synthesis of proteinase F in male mice.

[Production and purification of human interferons].

Human interferons have been classified into three types (Hu IFN-alpha, -beta, -gamma). Recently, relatively large amounts of their crude materials are being prepared using cell culture or recombinant DNA techniques. Consequently study or purification and clinical trials have been successfully performed.

A new esteroproteinase (proteinase F) from the submandibular glands of female mice.

One of the esteroproteinases present in the submandibular glands of female mice was purified and characterized. The enzyme, designated proteinase F in this report, had a pI value of 4.6 and a molecular weight of 27600, being comprised of two subunits of 10000 and 18000 daltons.

Characterization of two esteroproteases from the male mouse submandibular gland.

Three major esteroproteases, proteases A and D and P-esterase, obtained from the glands were studied kinetically and chemically; two (proteases A and D) were identified. Protease A is composed of a single subunit, molecular weight (27,600) similar to the native molecule (27,000); protease D consists of three subunits, approximate molecular weights of 9200, 7600 and 4600. P-esterase contains two subunits, approximate molecular weights of 7100 and 14,000.

Induction of epidermal growth factor by tri-iodo-L-thyronine in the submandibular glands of mice with testicular feminization.

The content of epidermal growth factor (EGF) as a high molecular weight complex (HMW-EGF) in the submandibular glands of mice was measured simply by a single radial immunodiffusion method. In female mice, the amount of HMW-EGF was increased 10-fold by tri-iodo-L-thyronine (T3) and 60-fold by 5 alpha-dihydrotestosterone (5 alpha-DHT). In mice with testicular feminization (Tfm), which are genetically deficient in androgen receptor, T3 but not 5 alpha-DHT increased the HMW-EGF from a non-detectable level to 5.

Induction of various androgen-dependent esteroproteases (trypsin-like and chymotrypsin-like enzymes) by tri-iodo-L-thyronine in the submandibular glands of female mice and mice with testicular feminization.

Trypsin-like and chymotrypsin-like esteroprotease isozymes of the mouse submandibular gland were separated by isoelectric focusing. In normal female mice the following pI-isozyme activities were found; pI-4.6, -5.

[A developmental study of narcissistic tendency (1) : women in pregnancy and confinement (author's transl)].

This study examined developmental changes in the narcissistic tendency in women by two questionnaires. The first questionnaire was given to 235 women in pregnancy (P groups) and the second to 49 women in confinement (C group) who were randomly chosen from among the 235 women surveyed first. Women when pregnant were more sensitive and more narcissistic compared with when not pregnant.

Effect of autonomic agents on the secretion of N-acetyl-beta-glucosaminidase of mouse submaxillary gland.

The secretion of N-acetyl-beta-glucosaminidase of mouse submaxillary gland into saliva was stimulated by norepinephrine and phenylephrine but not by pilocarpine and isoproterenol. The stimulative effects of the alpha-adrenergic agents were inhibited by alpha-blockers, phentolamine, and phenoxybenzamine. These results suggest that the secretion of the enzyme is regulated through alpha-adrenergic receptors.

Induction of androgen-dependent protease and serous-like granules by tri-iodothyronine in the submandibular gland of mice with testicular feminization.

Esteroprotease, an androgen-dependent enzyme of the mouse submandibular gland, was increased by injection of tri-iodothyronine (T3) in mice with testicular feminization (Tfm) which are genetically deficient in androgen receptors. Histochemical and electron microscopic studies also demonstrated increases of RNA and serous-like granules in cells of the convoluted tubules of the gland. These findings suggest that the esteroprotease gene in Tfm mice is normal and that T3 can induced both esteroprotease and serous-like granules independently of androgen.

Regulation of delayed-hypersensitivity response by the submandibular gland of male mice.

This study was performed to determine whether the removal of submandibular gland (SG) of mice influences the delayed-hypersensitivity (DH) response. The removal of the SG increased the weights of thymus and spleen and enhanced DH response, only in males and not in females. The injection of crude extract of male mouse SG returned the enhanced DH response of SG-ectomized males to the normal level whereas the injections of crude extracts of female or castrated male mouse SG was not affected.

Androgenic regulation of N-acetyl beta-glucosaminidase activity in the submandibular glands of mice.

N-Acetyl beta-glucosaminidase [beta-2-acetamido-2-deoxy-D-glucoside acetylamido-deoxyglucohydrolase; EC 3.2.1.

Sex difference in L-glutamine D-fructose-6-phosphate aminotransferase activity of mouse submandibular gland.

L-Glutamine D-fructose-6-phosphate aminotransferase (2-amino-2-deoxy-D-glucose-6-phosphate ketol-isomerase (amino-transferring), EC 5.3.1.

Quantitation of berberine chloride in human urine by use of selected ion monitoring in the field desorption mode.

A method is described for the microdetermination of berberine chloride in human urine by a field desorption mass spectrometry selected ion monitoring system using a deuterium labelled analogue of berberine chloride as an internal standard. Prior to the quantitation of berberine in human urine, the fundamental problems related to field desorption selected ion monitoring, such as quality of emitters, amounts of sample loading, and the programming rate of the emitter current, were statistically investigated in detail. Berberine chloride can be determined in a concentration of 10 ng ml-1 in human urine by the method described.

Effect of autonomic agents on the amount of androgen-dependent granules in convoluted tubular cells of the mouse submandibular gland.

The convoluted tubular cells of the male mouse submandibular gland contain many serous-like granules in their apical cytoplasm. The autonomic regulation of the secretory process of the contents of these granules was studied by the following two methods: (1) immunochemical method using an antiserum specific to the granular components; and (2) histometric observations using light and electron microscopes. The results obtained by these two methods were well in agreement.