Preparation of subcellular membranes from rat intestinal scrapings or isolated cells. Different Ca2+ binding, nonesterified fatty acid levels, and lipolytic activity.

Basolateral, brush-border, and Golgi-enriched subcellular membrane fractions, prepared from homogenates of rat small intestinal mucosa obtained by scraping, had unusually high concentrations of nonesterified fatty acids. These fatty acids appear to be responsible for the large amount of calcium binding, an effect that previously was shown to be reduced in vitamin D deficiency. In contrast, basolateral and Golgi membranes prepared from isolated cells had low levels of nonesterified fatty acids and calcium binding.

Localization of chloride secretion in rabbit colon: inhibition by anthracene-9-carboxylic acid.

The substituted aromatic compound anthracene-9-carboxylic acid (A-9-C) was used to inhibit active Cl- secretion by the epithelium of short-circuited rabbit distal colon. Tissues were mounted in Ussing chambers and stimulated to secrete Cl- by the addition of 1 mM dibutyryl adenosine 3',5'-cyclic monophosphate to the serosal bath. Results of 36Cl-flux measurements showed that the addition of 0.

Effect of in vitro fermentation using human faecal inoculum on the water-holding capacity of dietary fibre.

The water-holding capacities (WHC) of four sources of fibre were measured using dialysis membranes and osmotic-suction pressures of 45, 89 and 178 mosmol/1 (1, 2 and 4 atm). At all pressures, pectin had the highest WHC, followed by cabbage (Brassica oleracea) and lucerne (Medicago sativa) and then cellulose. A suction pressure of 89 mosmol/1 (2 atm) was used in the subsequent fermentation study since it had the lowest standard error of the mean and most closely approximated physiological conditions.

Synergistic effect of vitamin E and selenium in the chemoprevention of mammary carcinogenesis in rats.

The present study showed that vitamin E, although ineffective by itself, was able to potentiate the ability of selenium to inhibit the development of mammary tumors induced by dimethylbenz(a)anthracene (DMBA) in rats. Animals were maintained on a high-polyunsaturated fat (20% corn oil) diet in order to increase the degree of oxidant stress; additional selenium and/or vitamin E were present at a concentration of 2.5 and 1000 mg/kg of diet, respectively.

Clinical practice and the graduate nursing faculty.

When graduate faculty consider their responsibility to practice nursing, there needs to be an ensured framework within the school and university that will provide necessary support that includes opportunities and reward for demonstrated practice excellence. Setting realistic goals within an adequate time frame is essential. It does take a carefully formulated plan to balance practice, teaching, and scholarly work, and not make practice the sacrificial lamb when academic pressures increase.

Cloning of the mouse satellite DNA.

A stable recombinant clone was constructed by inserting a 1.5 kb mouse satellite DNA HindIII restriction fragment into the plasmid pBR-322. The cloned fragment according to both hybridisation data and restriction analysis seems to be identical with the major component of the mouse satellite DNA.

Cellular immune function in patients with cancer of the upper part of the gastrointestinal tract.

Lymphocytes of 30 patients were examined for cellular immune function one day prior to operation. In 11 patients the tests were repeated six weeks after surgery. In comparison with 26 healthy blood donors the E rosette values were strongly reduced in the patients group.