Solid-phase chemistry: Its principles and applications in clinical analysis.

A review is given of the development of solid-phase reaction systems (test papers, impregnated-fibre systems, multi-layer film systems) for rapid field and laboratory testing in clinical analysis.

Immobilized glutamate oxaloacetate transaminase. Steady state kinetic analysis and stability studies.

1. Glutamate oxaloacetate transaminase (L-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.

The preparation of nylon-tube-supported hexokinase and glucose 6-phosphate dehydrogenase and the use of the co-immobilized enzymes in the automated determination of glucose.

Triethyloxonium tetrafluoroborate was used to O-alkylate nylon-tube thus producing the imidate salt of the nylon which was further made to react with 1,6-diaminohexane. 2. Hexokinase (EC 2.

The preparation of several new nylon tube-glucose oxidase derivatives and their incorporation into the "reagentless" automated analysis of glucose.

Nylon tube was activated by alkylation with dimethyl sulfate and used for the immobilization of glucose oxidase. Lysine, hexamethylene diamine and polyethylene imine were also attached to activated nylon tube, and these nylon tube-spacer derivatives were reactivated with either glutaraldehyde or ethyl adipimidate for the subsequent coupling of glucose oxidase. The activities of all of the different nylon tube-glucose oxidase derivatives were compared by their incorporation into standard Technicon automated analysis systems.

Preparation of some immobilized linked enzyme systems and their use in the automated determination of disaccharides.

1. Glucose oxidase (EC 1.1.

The immobilization of enzymes on nylon structures and their use in automated analysis.

1. Glucose oxidase (EC 1.1.

The preparation and properties of yeast beta-fructofuranosidase chemically attached to polystyrene.

1. A process is described for the chemical attachment of an enzyme to the surface of a polystyrene matrix. 2.

Some changes in the reactivity of enzymes resulting from their chemical attachment to water-insoluble derivatives of cellulose.

1. Purified ficin was chemically attached to CM-cellulose, and partially purified ATP-creatine phosphotransferase was chemically attached to both CM-cellulose and p-aminobenzylcellulose. 2.

The kinetics of carboxymethylcellulose--ficin in packed beds.

1. The kinetics of the hydrolysis of benzoylarginine ethyl ester in packed columns of CM-cellulose-70-ficin and CM-cellulose-90-ficin were studied. 2.

The preparation and properties of ficin chemically attached to carboxymethylcellulose.

1. Purified ficin has been coupled to four CM-celluloses by reaction with their acid azide derivatives. Insoluble products containing 1.