Salivary Exosomal MicroRNA-486-5p and MicroRNA-10b-5p in Oral and Oropharyngeal Squamous Cell Carcinoma.

: The research aimed at evaluating the capacity of salivary exosomal miR-10b-5p and miR-486-5p for oral and oropharyngeal cancer detection. : The saliva samples were harvested from histopathological diagnosed oral and oropharyngeal squamous cell carcinoma patients and healthy volunteer subjects. The exosomes were isolated by differential ultracentrifugation and quantified by Nano Track Analysis.

Administration of 4‑hexylresorcinol increases p53‑mediated transcriptional activity in oral cancer cells with the p53 mutation.

The p53 mutation is inherent in over 50% of human cancers. In head and neck squamous cell carcinoma, the p53 mutation is associated with a poor prognosis. 4‑Hexylresorcinol (4HR) is a pharmacologic chaperone.

Salivary exosomal microRNAs as biomarkers for head and neck cancer detection-a literature review.

Background: MicroRNAs (miRs) are small, non-coding mRNA molecules which regulate cellular processes in tumorigenesis. miRs were discovered in extracellular environment and biological fluids, carrying marks of head and neck squamous cell carcinoma (HNSCC). They were also identified in abundance in salivary exosomes, in which they are protected by exosome lipid barrier against enzymatic injuries and therefore, the accuracy of exosomal miR-based cancer detection increase.

Giant follicular cyst with maxillary sinus and pterygomaxillary space extension.

Background: Dentigerous, follicular, cysts are developmental odontogenic cysts of reduced adamantine epithelium origin, associated with an impacted tooth. They are the second most frequent cystic pathology after inflammatory cysts. Although the frequency of dentigerous cysts associated with upper third molars is far lower than the mandibular ones, their complications are nothing but ordinary.

Principles of biomechanics in oral implantology.

Background And Aims: The principles of biomechanics comprise all the interactions between the body (tissues) and the forces acting upon it (directly or via different medical devices). Besides the mechanical aspects, the tissues response is also studied. Understanding and applying these principles is vital for the researchers in the field of oral implantology, but they must be equally known by the practitioners.

The Changes in Upper Airway Volume after Orthognathic Surgery Evaluated by Individual Segmentation on CBCT Images.

The aim of this CBCT study was to evaluate the postoperative volume changes of the rhinosinusal airway space, maxillary sinuses and nasal fossa, that occur after Le Fort I osteotomy, using individual segmentation of 3D virtual models. A number of 16 patients (with Class II and Class III malocclusions) who underwent a CBCT examination for orthognathic surgery at six month-interval between preoperative and postoperative evaluations were included. Patients with thickening of the sinus mucosa, craniofacial syndromes, maxillofacial trauma, rhinoplasty or other maxillofacial pathological conditions were excluded from the study.

Prof. Dr. Gheorghe Bilaşcu. Pro patria.

Gheorghe Bilaşcu came to Cluj from Budapest, where he enjoyed all the privileges reserved for the high-class people in the Hungarian capital city, in 1919. He renounced this comfortable existence in order to establish the in Cluj. When he made this choice, he was aware that he would sacrifice his life for a national ideal.

Early and long-term changes in the muscles of the mandible following orthognathic surgery.

Objectives: The aim of the present study is to evaluate the early and long-term postoperative dimensional changes of the muscles of the mandible in patients with orthognathic surgery for class II and class III malocclusions by using ultrasonography (US).

Material And Methods: Twenty-six patients who underwent bimaxillary orthognathic surgery for class II or class III malocclusions (14 and 12 patients, respectively) were ultrasonographically examined. The length, width, and cross-sectional area of the masseter and suprahyoid muscles were measured at three different time points: T (preoperatively), T (early postoperatively at 1 month after the surgery), and T (late postoperatively at 9 months).

Oral surgery for combined haemophilia A and B. The dos and don'ts presented in a clinical scenario.

Background: Haemophilia A (factor VIII deficiency), B (factor IX deficiency) and C (factor XI deficiency) are common genetic bleeding disorders. Most often they are caused by the absence or defective function of coagulation factors, causing inefficient blood clots.

Case Report: The present manuscript describes a rare case of a combined haemophilia A and B patient, who underwent several extractions.

Gheorghe Bȃrlea MD, PhD, pupil and collaborator of Professor Bilaşcu.

Being the first collaborator and assistant of Professor Gheorghe Bilaşcu, the founder of Cluj and National School of Dentistry, Dr. Gheorghe Bârlea kept very close to his master in developing the Dental Medicine in Cluj and in Romania, from 1908 to 1936. From the beginning of his career, he was involved in the establishment of the new Dental Clinic in the University of Superior Dacia as well as in the compilation of the teaching curriculum at the level of the avant-garde universities at that time.

Observational study of the bisphosphonate-related osteonecrosis of jaws.

Introduction: The bisphosphonate-related osteonecrosis of the jaw was first referred to in 2003. Bisphosphonates action is focused on the osteoclasts. The drastic inhibition of the osteoclastic function is harmful for the jaws which are the only bones of the human skeleton in relative contact with the external environment.

Estimation of the radiation dose for pediatric CBCT indications: a prospective study on ProMax3D.

Background: An increasing number of CBCT units and a wide variability of radiation doses have been reported in dentistry lately.

Aim: To estimate the effective, cumulative, and organ absorbed doses in children exposed to CBCT over 2 years.

Design: A prospective study was conducted in children who underwent CBCT diagnostic imaging with the ProMax3D machine.

Custom Implant for Reconstruction of Mandibular Continuity Defect.

Purpose: The purpose of this study was to compare the daily food intake rate and the rate of screw loosening between 2 groups of rabbits with mandibular continuity defects: custom implant (CI) group and 5-hole mini-plate group.

Materials And Methods: Two types of cylindrical implants were printed, and their physical strength was compared. In this study using rabbits, 1 group (n = 5) received a CI for the reconstruction of a mandibular continuity defect (CI group) and the other group (n = 5) received a 5-hole mini-plate without a bone graft (reconstruction plate [RP] group).

Bone regeneration is associated with the concentration of tumour necrosis factor-α induced by sericin released from a silk mat.

To understand the osteogenic effect of the middle layer of the silk cocoon, sericin was examined for its cellular effects associated with tumor necrosis factor-α (TNF-α) signaling in this study. The fragmented sericin proteins in the silk mat were evaluated for the TNF-α expression level in murine macrophages. The concentration of protein released from silk mats was higher in the outermost and the innermost layers than in the middle layers, and the protein released from the silk mat was identified as sericin.

Biography of Professor Cornel Tiberiu Opriş. Professional maturity.

Professor Cornel Tiberiu Opris was the founder and Chair of the Clinic and University Department of Oral and Maxillofacial Surgery in Cluj, after the Education Reform of 1948. The article illustrates how the founder of these institutions led a valiant struggle for obtaining and arranging a location for the newly established Faculty of Dentistry, within the Institute of Medicine and Pharmacy. Professor Cornel Tiberiu Opriş established himself as the most prodigious researcher at the Faculty for over a quarter-century, until his retirement, introducing his original conception in the therapeutic and surgical field.

Evaluation of the Biocompatibility of New Fiber-Reinforced Composite Materials for Craniofacial Bone Reconstruction.

This study aims to assess the biocompatibility of new advanced fiber-reinforced composites (FRC) to be used for custom-made cranial implants. Four new formulations of FRC were obtained using polymeric matrices (combinations of monomers bisphenol A glycidylmethacrylate [bis-GMA], urethane dimethacrylate [UDMA], triethylene glycol dimethacrylate [TEGDMA], hydroxyethyl methacrylate [HEMA]) and E-glass fibers (300 g/mp). Every FRC contains 65% E-glass and 35% polymeric matrix.

Professor Gheorghe Bilaşcu's contribution to the development of science and culture in Romania.

When speaking about Professor Gheorghe Bilaşcu (1863-1926) and his major contribution to the establishment of Romanian medical education in Cluj, he should be considered not only in terms of scientist and creator of the Dental School, but also through his commitment to the development of science and culture in Romania. A wealthy dentist in Budapest where he graduated from the Dental School, he supported a lot of Romanian students to attend schools and universities in the Budapest, thus contributing to the development of culture in his own country. Finally, he left his private practice in the Capital of Hungary to come to Cluj to support the efforts of building the Dental School and profession in Romania.

Antibacterial coating on biocomposites for cranio-facial reconstruction.

Background And Aims: Despite the fact that implants are sterilized, antiseptic techniques are applied and systemic antibiotics are routinely administered prior to and after craniofacial surgery, infection rates between 3% and 40% are still reported for alloplastic implants, urging for implant removal. The present study focuses on the development of a fiber-reinforced composite (FRC) implant for craniofacial reconstruction with antimicrobial properties.

Methods: A new fiber-reinforced composite coated with gentamicin was developed and tested for bacterial adherence and antibacterial efficiency, using two of the most involved bacterial strains in the postoperative infections: Staphylococcus aureus and Pseudomonas aeruginosa.

The ultrasonographic diagnosis of cystic cervical lesions: a pictorial essay.

The importance of ultrasonographic (US) examination in the evaluation of the superficial structures of the head and neck region is increasing due to the widespread availability of modern equipment (such as high-frequency transducers) and modern techniques. In the case of a cervical lesion, ultrasound is usually the first imaging method used to assess the changes. First of all, US is capable of differentiating between cystic and solid structures, and based on the US appearance correlated with the age of the patient, location and growing pattern, the appropriate diagnosis is possible with a high accuracy.

The spirit of professor Iacob Iacobovici in the development of education in dentistry and maxillofacial surgery.

Founder of the Surgical Clinic in Cluj-Napoca and of Medical Education in Romanian, Rector of Dacia Superior University, Professor Iacob Iacobovici was one of the outstanding medical personalities in the first half of the twentieth century, in Romania. His scientific contributions have been recognized by the European great personalities of his time. A remarkable bright mind, with an overarching comprehension of things, Professor Iacob Iacobovici contributed, in an essential way, to the diversification of surgical education, stimulating the emergence of new specialties.

Micro-CT and histological analysis of Ti6Al7Nb custom made implants with hydroxyapatite and SiO2-TiO2 coatings in a rabbit model.

Background And Aim: Bone defect reconstruction in the maxillofacial area comes as a necessity after traumatic, oncological or congenital pathology. Custom made implant manufacturing, such as selective laser melting (SLM), is very helpful when bone reconstruction is needed. In the present study we assessed the osseointegration of custom made implants made of Ti6Al7Nb with two different coatings: SiO2-TiO2 and hydroxyapatite, by comparing the bone mineral density (BMD) measured on micro-CT and the histological mineralized bone surrounding the implants.

Selective laser melted titanium implants: a new technique for the reconstruction of extensive zygomatic complex defects.

The restoration of extensive zygomatic complex defects is a surgical challenge owing to the difficulty of accurately restoring the normal anatomy, symmetry, proper facial projection and facial width. In the present study, an extensive post-traumatic zygomatic bone defect was reconstructed using a custom-made implant that was made with a selective laser melting (SLM) technique. The computer-designed implant had the proper geometry and fit perfectly into the defect without requiring any intraoperative adjustments.

Pedicled buccal fat pad flap as a reliable surgical strategy for the treatment of medication-related osteonecrosis of the jaw.

Purpose: The purpose of this study was to evaluate the coverage of the pedicled buccal fat pad flap (PBFP) and the long-term results of this treatment in patients with medication-related osteonecrosis of the jaw (MRONJ).

Patients And Methods: Ten patients (2 men and 8 women; average age, 72.9 yr old) diagnosed with MRONJ were selected.

Cisplatin and 4-hexylresorcinol synergise to decrease metastasis and increase survival rate in an oral mucosal melanoma xenograft model: a preliminary study.

The present study was undertaken to examine the effects of cisplatin plus 4-hexylresorcinol (4-HR) combination therapy on oral mucosal melanoma (OMM) using cultured primary OMM cells in a tumour xenograft model. Cultured primary OMM cells were used for the MTT assay and DNA microarray. OMM cells were implanted into the submandibular glands of nude mice.

Restoration of a peri-implant defect by platelet-rich fibrin.

Objective: The objective of this study was to evaluate the application of platelet-rich fibrin (PRF) into a peri-implant defect in vivo.

Study Design: Eight New Zealand white rabbits were used for this study. Two peri-implants with defects sized 3.