Publications by authors named "Horacio G Nastri"
CDR: complementarity determining region; FACS: fluorescence-activated cell sorting; k: association rate; k: dissociation rate; K: dissociation constant; scFv: single-chain variable fragment; SPR: surface plasmon resonance.
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- The study investigates the creation of a diverse antibody library from a single healthy individual, challenging the traditional view that more donors equal greater diversity.
- The research emphasizes that the quantity of lymphocytes is more crucial than the number of donors in producing a functional library.
- A high-quality phage display library was constructed, utilizing efficient extraction and amplification methods, and validated through next-generation sequencing, demonstrating successful functionality against various therapeutic targets.
Combinatorial libraries of rearranged hypervariable V(H) and V(L) sequences from nonimmunized human donors contain antigen specificities, including anti-self reactivities, created by random pairing of V(H)s and V(L)s. Somatic hypermutation of immunoglobulin genes, however, is critical in the generation of high-affinity antibodies in vivo and occurs only after immunization. Thus, in combinatorial phage display libraries from nonimmunized donors, high-affinity antibodies are rarely found.
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