A modified indirect cast post and core technique.

An indirect post and core technique in which the core is waxed and invested with the die has been described. The post and core are cast directly into the invested die, which has the advantage of an accurate casting of the post preparation obtained with less laboratory time and without the disadvantages of retrieving an intact wax pattern. This technique is particularly suited for long, narrow post preparations; short preparations where precise fit is necessary for retention; and multiple adjacent post preparations.

Purification and characterization of posterior pituitary calmodulin and its activation of neurosecretosome Ca2+ + Mg2+-ATPase activities.

Calmodulin was isolated as an electrophoretically homogeneous protein from bovine posterior pituitary glands. The yield indicated that this gland is a particularly rich source. Purified bovine posterior pituitary calmodulin and bovine brain calmodulin had identical electrophoretic mobilities on 10% and 12% polyacrylamide gels.

Cyclic nucleotides and the release of vasopressin from the rat posterior pituitary gland.

When rat posterior pituitary glands were stimulated by a high concentration of potassium, a peak of cyclic AMP and a peak of cyclic GMP were detected after 0.5 min and 1 min, respectively, whereas the rate of release of vasopressin was maximal only after 2 min. When calcium was omitted from the medium, no significant changes in cyclic nucleotide levels were found and the vasopressin release remained at the basal rate.

Normal infant after combination chemotherapy including teniposide for Burkitt's lymphoma in pregnancy.

A 32-year-old woman was treated for Burkitt's lymphoma diagnosed during the 22nd week of pregnancy. Chemotherapy consisting of doxorubicin (Adriamycin), vincristine, teniposide (VM-26), cyclophosphamide, and prednisolone (AVTEP) was given each 2.5 to 3 weeks from the time of the diagnosis until delivery in the 37th week.

Identification of aspirinase with one of the carboxylesterases requiring a thiol group.

Aspirin-hydrolysing activity in guinea-pig liver is located mainly in the microsomal fraction. This activity was found by electrophoresis to be due to a single carboxylesterase band, out of 12 bands revealed with alpha-naphthyl acetate as substrate. The activity is inhibited completely and irreversibly by the carboxylesterase inhibitor bis-(-4-nitrophenyl) hydrogen phosphate, and also by thiol-blocking reagents.

Hydroxy analogues of oxytocin and of lysine-vasopressin.

1 Synthetic analogues of oxytocin and of lysine-vasopressin with an hydroxyl group in either the L ro D configuration replacing the primary amino group have been tested for biological activity.2 [1-(L-2-Hydroxy-3-mercaptopropanoic acid)] oxytocin ([L-Hmp(1)]oxytocin) was 1.5 to 2 times more potent than oxytocin on the rat uterus in situ, the rat mammary strip and the rat mammary gland in situ and 3 times more potent on the rat isolated uterus.

Salmonellosis associated with late rupture of an aortofemoral bypass graft.

Salmonella infection is a rare cause of rupture of the atherosclerotic abdominal aneurysm. A case never previously reported is presented in which salmonella infection occurred involving an aortofemoral bypass graft which had been inserted 10 years previously. The infection caused a leak in the region of the graft which presented as a haematoma in the thigh.

Subcellular fractionation by centrifugation of homogenates of the neural lobe of the bovine pituitary gland: identification of different pools of hormone in the homogenate and isolation of neurosecretosomes.

1. Homogenates of bovine pituitary neural lobe tissue were subjected to differential centrifugation. Six subcellular fractions (I-VI) were obtained and the distribution among them of various cell organelles was studied by means of markers.

Co-operative binding of oxytocin to bovine neurophysin II.

The interaction of oxytocin with bovine neurophysin II in 0.1 M-sodium phosphate, pH 5.8, was investigated by equilibrium-dialysis and sedimentation studies.

Isolation and protein composition of membranes of neurosecretory vesicles and plasma membranes from the neural lobe of the bovine pituitary gland.

1. Homogenates of neural lobes of bovine pituitary glands were fractionated by differential ultracentrifugation. 2.