Thymopentin treatment in genital warts of long duration.

The immunomodulatory effect of thymopentin as therapy in genital warts of long duration and the proliferative responses of the patient's peripheral blood mononuclear cells were investigated in this pilot study. The observations in 6 patients suggest that subcutaneous injections of thymopentin (50 mg) beneficially influence the systems of therapy-resistant genital warts. The small number of patients and controls used in the assessment of the proliferation responses allows only a descriptive analysis of the results, but definitive trends can be observed in the Con A- and PHA-induced proliferation tests.

Development of a standardized analysis strategy for basic drugs using ion-pair extraction and high-performance liquid chromatography - VII. Determination of drugs in plasma.

A standardized analysis strategy for basic drugs which had been applied previously to pharmaceutical dosage forms, cosmetics and saliva has been applied to plasma. Low extraction recoveries that were obtained at first were shown by protein binding studies to be due to interactions between plasma proteins, the drug and the organic ion-pairing reagent. These interactions could be avoided by removal of the plasma proteins from the sample prior to addition of the counterion solution.

Development of a standardized analysis strategy for basic drugs, using ion-pair extraction and high-performance liquid chromatography. VI. Drug level determination in saliva.

Assay methods for measuring saliva levels of carbamazepine and its active metabolite, and of amidopyrine are developed using a standardized analysis strategy. Both assay methods include ion-pair extraction of the analytes with octylsulphate as the counter-ion and chromatography on a CN-bonded phase using a hexane--dichloromethane--acetonitrile--propylamine mixture as the mobile phase. Both methods are applied to patient samples.

Development of a standardized analysis strategy for basic drugs, using ion-pair extraction and high-performance liquid chromatography - III. Analysis of pharmaceutical dosage forms.

The usefulness of the standardized analysis strategy previously described for the determination of basic drugs in pharmaceutical dosage forms is evaluated. Several examples which typify experience in applying the strategy are reported. Ion-pair extraction techniques are compared with each other and with a classical extraction method in terms of their efficiency.

Development of a standardized analysis strategy for basic drugs, using ion-pair extraction and high-performance liquid chromatography - II. Selection of preferred HPLC-systems.

A test set of 100 basic drugs has been chromatographed on 16 preselected HPLC-systems using four different types of stationary phase (Si-, NH2-, CN- and C18-). A numerical treatment of the chromatographic data, based on the discriminating power concept, results in the selection of two preferred HPLC systems for basic drugs. both using the CN-bonded phase.

Development of a standardized analysis strategy for basic drugs using ion-pair extraction and high-performance liquid chromatography - I. Philosophy and selection of extraction technique.

The first of a series of papers on the development of a standardized analysis strategy for basic drugs explains the possible advantages and philosophy of the strategy. The scheme uses ion-pair extraction with direct injection of the extracts into an HPLC system emanating, from two previously-selected systems. The extraction efficiency of sodium-n-octylsulphate as the ion-pairing reagent is compared with that of di(2-ethylhexyl) phosphoric acid (previously shown to be generally applicable to the extraction of basic drugs).