Influence of a cod liver oil diet in healthy and insulin-dependent diabetic volunteers on fatty acid pattern, inhibition of prostacyclin formation by low density lipoprotein (LDL) and platelet thromboxane.

Ten healthy and twenty diabetic volunteers (type 1) received 15 capsules (à 450 mg) cod liver oil for 2 weeks daily in addition to a "normal" diet. The levels of eicosapentaenoic acid in the plasma phospholipids of both groups were increased after the treatment. The inhibition of the prostacyclin formation by LDL was diminished when the LDL was isolated after the treatment in comparison to LDL taken in the same concentration and from the same donors before it.

[Sex-specific differences in food intake, body weight and parameters of lipid metabolism (HDL-cholesterol, total cholesterol and triglycerides) in rats under various feeding conditions].

We compared the food intake, body weight as well as several parameters of the lipid metabolism in male and female rats fed with a diet high in carbohydrate content (HCD) or high in fat content (HFD). The absolute food intake was higher in male than in female animals. With regard to the relative food intake (per 1 000 g body weight), the sex-specific differences could be demonstrated only in the HCD rats.

Thromboxane B2 (TXB2) formation in clotting whole blood of healthy and diabetic humans in vitro.

We investigated the ability of platelets from two groups of diabetics type I and two groups of healthy volunteers matched of age to generate thromboxane B2 (TXB2) during spontaneous clotting of whole blood. The serum concentration of TXB2, reflecting the ability of the platelets to generate TXA2 during clotting, was measured by gas liquid chromatography. Platelets from old diabetics with more than 40 years duration of diabetes mellitus formed significantly less TXB2 than those from old healthy controls.

Influence of a cod liver oil diet in diabetics type I on fatty acid patterns and platelet aggregation.

The influence of an eicosapentaenoic acid rich diet containing only 6,8 g cod liver oil daily for 2 weeks in 20 type I diabetics on fatty acid pattern in serum, platelet aggregation and thiobarbituric acid-reactive substances in serum were studied. There were increases in eicosapentaenoic acid portions in triglycerides, cholesterol esters and phospholipids of serum. This was associated with an inhibition of the platelet hyperaggregation, whereas platelet hyporeactivity is shifted to normal.

[Results of linseed oil and olive oil therapy in hyperlipoproteinemia patients].

Only few informations exist on the effect of different oils and the transformation of these precursor fatty acids to prostaglandins in patients with hyperlipoproteinemia. Therefore we investigated the impact of linseed oil and olive oil intake resp. on serum lipoprotein levels, platelet aggregation and fatty acid pattern of serum phospholipids and triglycerides resp.

Negative correlation of eicosapentaenoic acid and lipid accumulation in hepatocytes of diabetics.

In 130 type I and II diabetics with normal serum lipids and in 98 diabetics with type IIa-V hyperlipoproteinemia (HLP), liver biopsies were performed if clinically indicated. During histological examinations of one half of the biopsy specimen lipid droplet size was classified into 4 categories, which were proved by morphometric studies, independent of the amount of fat. From the remaining part the fatty acid composition of triglycerides was analyzed by gas liquid chromatography.

[Fatty acid patterns of triglycerides in serum and skeletal muscles of rabbits on a cholesterol diet].

In 18 rabbits a hypercholesterolaemia was produced by a 12-week dietetic load with cholesterol. The hypercholesterolaemic rabbits had increased proportions of saturated and simply unsaturated fatty acids in the triglycerides of the serum and the skeletal muscle. The content of eicosapentaenic acid was lowered.

[Fatty acid composition of triglycerides in inflammatory liver diseases in diabetics with and without hyperlipoproteinemia].

In 228 patients with diabetes mellitus (130 diabetics without and 98 diabetics with hyperlipoproteinaemia) percutaneous liver punctures after Menghini as well as biopsies of the subcutaneous fatty tissue were carried out. From the biopsy specimens and from serum the fatty acid pattern of triglycerides was estimated. In 87 patients with chronic aggressive hepatitis and 37 patients with fibrosis no differences in the fatty acid composition could be found.

Decrease of eicosapentaenoic acid in fatty liver of diabetic subjects.

Simultaneous biopsies of liver and subcutaneous adipose tissue have been carried out in 228 patients with diabetes mellitus. In liver triglycerides a marked variability of the fatty acid pattern in relation to fatty degeneration of liver parenchyma has been confirmed. In adipose tissue fatty acid pattern was relatively constant.

The fatty acid pattern of triglycerides in liver, adipose tissue and serum of diabetics with hyperlipoproteinemia before and during clofibrate treatment.

In 20 diabetic inpatients with type IIb, III, IV and V hyperlipoproteinemia (HPL) the triglyceride fatty acid pattern (TFAP) of serum, adipose tissue and liver biopsy specimens before and after one year of clofibrate treatment has been determined by gas-liquid chromatography. Compared to previous results which revealed a correlation between fat droplet size and the TFAP in liver parenchyma cells, remarkable changes were observed after long-term therapy. In adipose tissue, only linoleic acid increased significantly from 8.

The triglyceride fatty acid pattern in arterial wall, adipose tissue and serum of amputated diabetic subjects.

The triglyceride fatty acid pattern [TFAP] in arterial wall, adipose tissue and serum has been estimated in diabetic subjects by gas-liquid chromatography simultaneously. The samples were taken shortly before or during femoral amputation performed under halothane anaesthesia. In addition, in some probands the fatty acids of cholesterol esters of vascular walls have been obtained.

The fatty acid composition of triglycerides in arteries, depot fat and serum of amputated diabetics.

The triglyceride fatty acid pattern (TFAP) in arterial wall, adipose tissue and serum in vivo has been estimated in amputated diabetic subjects by gas-liquid chromatography simultaneously. Besides, in some probands the fatty acid pattern of cholesterol esters of vessel walls has been obtained. In arterial wall the percental content of lauric, myristic, myristoleic, palmitoleic, stearic, oleic and linolenic acid was high and that of palmitic and linoleic acid was low, when compared to serum, whereas eicosatrienoic, arachidonic and eicosapentaenoic acid were of similar magnitude.