Sensitive Quantitative In Vivo Assay for Evaluating the Effects of Biomolecules on Hair Growth and Coloring Using Direct Microinjections into Mouse Whisker Follicles.

Many people suffer from hair loss and abnormal skin pigmentation, highlighting the need for simple assays to support drug discovery research. Current assays have various limitations, such as being in vitro only, not sensitive enough, or unquantifiable. We took advantage of the bilateral symmetry and large size of mouse whisker follicles to develop a novel in vivo assay called "whisker follicle microinjection assay".

Identification of Cancer Driver Genes by Integrating Multiomics Data with Graph Neural Networks.

Cancer is a heterogeneous disease that is driven by the accumulation of both genetic and nongenetic alterations, so integrating multiomics data and extracting effective information from them is expected to be an effective way to predict cancer driver genes. In this paper, we first generate comprehensive instructive features for each gene from genomic, epigenomic, transcriptomic levels together with protein-protein interaction (PPI)-networks-derived attributes and then propose a novel semisupervised deep graph learning framework GGraphSAGE to predict cancer driver genes according to the impact of the alterations on a biological system. When applied to eight tumor types, experimental results suggest that GGraphSAGE outperforms several state-of-the-art computational methods for driver genes identification.

Expression dynamics of lymphoid enhancer-binding factor 1 in terminal Schwann cells, dermal papilla, and interfollicular epidermis.

Background: Transcription factor lymphoid enhancer-binding factor 1 (LEF1) is a downstream mediator of the Wnt/β-catenin signaling pathway. It is expressed in dermal papilla and surrounding cells in the hair follicle, promoting cell proliferation, and differentiation.

Results: Here, we report that LEF1 is also expressed all through the hair cycle in the terminal Schwann cells (TSCs), a component of the lanceolate complex located at the isthmus.

Triazine-Based Covalent DNA-Encoded Libraries for Discovery of Covalent Inhibitors of Target Proteins.

Since ibrutinib was approved by the FDA as an effective monotherapy for chronic lymphocytic leukemia (CLL) and multilymphoma, more and more FDA-approved covalent drugs are coming back into the market. On this occasion, the resurgence of interest in covalent drugs calls for more hit discovery techniques. However, the limited numbers of covalent libraries prevent the development of this area.

Large-scale isolation of functional dermal papilla cells using novel surface marker LEPTIN Receptor.

Dermal papilla (DP) cells regulate hair follicle epithelial cells and melanocytes by secreting functional factors, playing a key role in hair follicle morphogenesis and hair growth. DP cells can reconstitute new hair follicles and induce hair regeneration, providing a potential therapeutic strategy for treating hair loss. However, current methods for isolating DP cells are either inefficient (physical microdissection) or only applied to genetically labeled mice.

Anti-inflammatory Constituents from Caulis Trachelospermi.

Caulis Trachelospermi, the stems with leaves of , is a well-known herbal drug of the Apocynaceae family recorded in the Chinese pharmacopeia and used for the treatment of inflammation-related diseases by ethnic minorities of China. The mechanism of anti-inflammatory activity and responsible constituents of have not been well elucidated in previous studies. Preliminary investigation showed that both the water and the ethyl ester extracts of exhibited potent inhibitory activity on nitric oxide (NO) production using lipopolysaccharide (LPS)-stimulated murine macrophages.

Ligulaveitnoid A, a new phenylpropanoid from rhizomes and roots of .

A new phenylpropanoid, ligulaveitnoid A (), along with four known compounds, ()-2,3-dihydroconiferyl -coumarate (), dihydroconiferyl ferulate (), 4-hydroxy-3-methoxybenzaldehyde () and ()--coumaric acid () were isolated from rhizomes and roots of . All the structures of compounds were identified by the interpretation of their spectroscopic data and comparison with those reported in the literature. The anti-inflammatory activity of the isolates was examined for their inhibitory effects on LPS-induced NO production in macrophage RAW264.

Potential anti-diabetic isoprenoids and a long-chain δ-lactone from frangipani (Plumeria rubra).

A decoction of Plumeria rubra flowers has been used traditionally for the treatment of diabetes in China and Mexico. Chemical investigations on the bioactive constituents of these flowers led to the isolation of 30 compounds, including the four new compounds, one iridoiod (1), two triterpenoids (4, 5), and a long-chain δ-lactone (16). In addition, 26 known compounds (2, 3, 6-15, 17-30) are also reported.

Chemical constituents from the barks of and their PTP1B inhibitory activity.

One new long-chain ester derivative of trans-ferulic acid and one natural tirucallane-type triterpenoid , together with forty known compounds (), were isolated from the barks of . Their structures were established on the basis of spectroscopic data interpretation. Compounds , , , , showed significant inhibitory activities against PTP1B with IC values of 13.

Chemical constituents from the leaves of .

Six compounds, benzyl 3---D-glucopyranosyl-7-hydroxybenzoate (), spathulenol (), 1,7,8-trihydroxy-2-naphtaldehyde (), quercetin (), astragalin () and 2-methoxy-4-(2-propenyl)phenyl β-D-glucoside (), were isolated from the leaves of L. The structure elucidation of compound was discussed in detail based on its 2D-NMR data. Compound showed weak cytotoxicity against the cell lines of T-24, NCI-H460, HepG2, SMMC-7721, CNE, MDA-MB-231 and B16F10 with the inhibition rates from 10.