Important ecophysiological roles of Nocardiopsis in lignocellulose degradation during aerobic compost with humic acid addition.

Improving lignocellulose degradation and organic matter conversion in agricultural and livestock wastes remains a great challenge. Here, the contribution of humic acid (HA) to lignocellulose degradation was investigated, focusing on the abundance of key microbial species and carbohydrate-active enzymes during aerobic composting. The results demonstrated that the addition of HA not only increased the complexity of the microbial network, but also enhanced the positive interaction between microorganism.

Analysis of Pickled Cucumber Products, Based on Microbial Diversity and Flavor Substance Detection.

Changes to the microbial community during pickled cucumber fermentation were studied using the 16S rDNA technique. The changes of volatile organic compounds (VOCs) during pickled cucumber fermentation were studied by gas chromatograph-ion mobility spectrometry. At the phylum level, and were the dominant flora in the natural fermentation group, and were the dominant flora in the added-bacteria fermentation group.

Response characteristics of flax retting liquid addition during chicken manure composting: Focusing on core bacteria in organic carbon mineralization and humification.

To explore the applicability of flax retting liquid (FRL) addition, the physicochemical properties, microbial community structure and function, carbon conversion and humus (HS) formation were assessed during chicken manure (CM) aerobic composting. Compared with the control group, the addition of FRL increased the temperature at thermophilic phase, while the microbial mass carbon content (MBC) in SCF and FRH groups raised to 96.1 ± 0.

Analysis of Microbial Diversity and Metabolites in Sauerkraut Products with and without Microorganism Addition.

The microbial compositions and metabolites of fermented sauerkraut with and without the addition of microorganisms have been compared. The OTU clustering, nonvolatile compounds, volatile compounds and associations between bacterial taxa and metabolites were analyzed by 16S rRNA high-throughput sequencing technology, ultra performance liquid chromatography (UPLC), gas chromatography ion mobility mass spectrometry (GC-IMS) and the O2PLS model studies. The results showed that at the phylum level, the microbial species in the four sauerkraut types consisted mainly of the phyla and , but different modes of microbial addition formed their own unique microbial communities.

Aerobic composting with sauerkraut fermentation waste water: Increasing the stability and complexity of bacterial community and changing bacterial community assembly processes.

Aerobic composting renders the sauerkraut fermentation waste water harmless while adding soluble nutrients. Unravelling the bacterial community assembly processes, changes in community robustness and community cohesion and the relationship between them under composting treatment of sauerkraut fermentation waste water is an interesting topic. Sauerkraut fermentation waste water was used for composting, which increased bacterial linkages, community robustness, competitive behaviour during warming periods and cooperative behaviour during cooling periods, and the control of community assembly processes shifts from deterministic processes (variable selection) to stochastic processes (decentralised limitation).

Characterization of Dextran Biosynthesized by Glucansucrase from and Their Potential Biotechnological Applications.

Glucansucrase was purified from . The glucansucrase exhibited maximum activity at pH 5.5 and 30 °C.

Aerobic composting of chicken manure with penicillin G: Community classification and quorum sensing mediating its contribution to humification.

Chicken manure containing antibiotics is a hazardous biological waste. The purpose of our study was to investigate how different concentrations of penicillin G alter the bacterial community to affect humification during aerobic composting of chicken manure. The effect of quorum sensing on the bacterial community was also evaluated.

Analysis of the structure and properties of dextran produced by Weissella confusa.

An EPS produced by Weissella confusa H2 was purified through Sephadex G-100, and the preliminary structure characteristics and biological activities of H2 EPS were analyzed. Molecular mass of purified H2 EPS was 2.705 × 10 Da as measured with gel permeation chromatography (GPC).

Detection of the microbial diversity and flavour components of northeastern Chinese soybean paste during storage.

A combination of 16S rDNA and GC-IMS was used to study the changes in the composition of microorganisms and volatile organic compounds (VOCs) during the storage of northeastern Chinese soybean paste. Firmicutes and Actinobacteriota dominated the microbial communities of the soybean paste at the phylum level, bacterial profiles of different samples were different at genus level. Fifty-one VOCs were identified from soybean paste, most of which existed in the early storage stage.

Optimization of cultivation strategy and medium for bacteriocin activity of HDX-2.

A lactic acid bacteria (LAB) isolated from pickled Chinese cucumber was screened for bacteriocin production. The strain was identified to be HDX-2. Based on the Plackett-Burman (PB) experiment, glucose, Ca, and initial pH were found to be the most significant parameters of bacteriocin production.

Purification and characterization of an exopolysaccharide from Leuconostoc lactis L2.

An exopolysaccharide (EPS)-producing bacterium was isolated from the homemade fermentation product of hami melon (Cucumis melo var. saccharinus) and identified as Leuconostoc lactis L2 mainly based on 16S rDNA sequence analysis and the API 50 CHL test. The monosaccharide composition of Leu.

Monoraphidium sp. HDMA-20 is a new potential source of α-linolenic acid and eicosatetraenoic acid.

Background: ω-3 polyunsaturated fatty acids (PUFAs) are synthesized from α-Linolenic acid (ALA, C18:3ω3) and play important roles in anti-inflammatory and antioxidant responses in mammal cells. ALA is an essential fatty acid which cannot be produced within the human body and must be acquired through diet. The purpose of this study was to evaluate the potential of a novel microalgal strain (HDMA-20) as a source of ω-3 PUFAs including ALA and eicosatetraenoic acid (ETA, C20:4ω3).

Isolation of a novel strain of Monoraphidium sp. and characterization of its potential for α-linolenic acid and biodiesel production.

α-Linolenic acid (ALA) is an essential fatty acid which cannot be synthesized de novo in mammals and must be ingested regularly in the diet. In this study, a microalgal strain named HDMA-11 was isolated from Lake Ming, China, and was found to accumulate a high ALA content (39.2% of total lipids).

A genome shuffling-generated Saccharomyces cerevisiae isolate that ferments xylose and glucose to produce high levels of ethanol.

Genome shuffling is an efficient approach for the rapid improvement of industrially important microbial phenotypes. This report describes optimized conditions for protoplast preparation, regeneration, inactivation, and fusion using the Saccharomyces cerevisiae W5 strain. Ethanol production was confirmed by TTC (triphenyl tetrazolium chloride) screening and high-performance liquid chromatography (HPLC).

[Fermentation optimization by response surface methodology for enhanced production of beta-glucosidase of Aspergillus niger HDF05].

In order to obtain high beta-glucosidase productivity, we optimized the fermentation parameters for beta-glucosidase production by Aspergillus niger HDF05. First, we screened the important parameters by Plackeet-Burman design. Second, we used the path of steepest ascent to approach to the biggest response region of parameters affecting beta-glucosidase production.

[Optimization of xylose fermentation for ethanol production by Candida shehatae HDYXHT-01].

Plackett-Burman (PB) design and central composite design (CCD) were applied to optimize of xylose fermentation for ethanol production by Candida shehatae HDYXHT-01. The PB results showed that (NH4)2SO4, KH2PO4, yeast extract and inoculum volume were the main affecting factors. With ethanol productivity as the target response, the optimal fermentation was determined by CCD and response surface analysis (RSM).

[Construction of integrative vector for xylulokinase gene and its overexpression in Saccharomyces cerevisiae].

Objective: An integrative vector of Saccharomyces cerevisiae for xylulokinase gene expression was constructed to overexpress xylulokinase activity.

Methods: On the basis of plasmid p406ADH1, 4 components were integrated, which were KanR gene as G418 resistant marker, ADH1 terminator fragment, xylulokinase gene from Saccharomyces cerevisiae W5 and 18S rDNA sequence for homologous recombination. After enzyme digestion and ligation, high copy recombinant expression vector pCXS-RKTr was constructed.

Statistical optimization of xylitol production from corncob hemicellulose hydrolysate by Candida tropicalis HDY-02.

The statistical experimental designs were adopted to optimize the culture medium in xylitol production by Candida tropicalis HDY-02 with corncob hemicellulose hydrolysate as substrate. In the first step, Plackett-Burman design was used for screening the important variables. KH(2)PO(4), yeast extract, (NH(4))(2)SO(4) and MgSO(4)·7H(2)O were found to significantly affect xylitol yield.

Paracin 1.7, a bacteriocin produced by Lactobacillus paracasei HD1.7 isolated from Chinese cabbage sauerkraut, a traditional Chinese fermented vegetable food.

Objects: To purify and characterize bacteriocin produced by L. paracasei HD1.7.

Sugarcane bagasse mild alkaline/oxidative pretreatment for ethanol production by alkaline recycle process.

In order to decrease the alkali and water consumptions in the sugarcane bagasse alkaline/oxidative pretreatment for ethanol production, an alkaline recycle process was carried out. Two recycles of NaOH/H2O2 pretreatment did not decrease the pretreatment and enzymatic hydrolysis efficiencies and the consumptions of NaOH and water would be saved by 26% and 40%, respectively. A simultaneous saccharification and fermentation (SSF) culture with pretreated bagasse as substrate was developed giving 25 g ethanol l(-1) with a yield of 0.

Fermentation of pretreated sugarcane bagasse hemicellulose hydrolysate to ethanol by Pachysolen tannophilus.

Sugarcane bagasse hemicellulose hydrolysates, pretreated by either over-liming or electrodialysis and, supplemented with nutrient materials, were fermented to ethanol using Pachysolen tannophilus DW06. Compared with detoxification by over-liming, detoxification by electrodialysis decreased the loss of sugar and increased the acetic acid removal, leading to better fermentability. A batch culture with electrodialytically pretreated hydrolysate as substrate was developed giving 21 g ethanol l(-1) with a yield of 0.