MALAT1 accelerates proliferation and inflammation and suppresses apoptosis of endometrial stromal cells via the microRNA-142-3p/CXCR7 axis.

MALAT1, microRNA (miR)-142-3p, and CXCR7 are critical molecules mediating endometriosis progression, and their correlation in endometriosis has been barely discussed. Thus, this research sought to survey the impact of MALAT1 on endometrial stromal cell (ESC) proliferation, apoptosis, and inflammation via miR-142-3p/CXCR7 axis to promote explorations on endometriosis. In endometrial tissues and ESCs, CXCR7 expression was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blot analysis and miR-142-3p and MALAT1 expression by qRT-PCR.

CsA Promotes XIST Expression to Regulate Human Trophoblast Cells Proliferation and Invasion Through miR-144/Titin Axis.

In our previous study, we revealed that Cyclosporin A (CsA) could inhibit miR-144 expression to regulate proliferation and invasion of human trophoblast (HT) cells through miR-144 targeting titin. This partially demonstrated the mechanism by which CsA promotes titin expression to increase the vitality of HT cells. However, the mechanism by which CsA inhibits miR-144 expression remains to be investigated.

[Effect of emodin lipid nano-microbubble on MAPK signal pathway and inflammation cytokine in AT-II cells by mechanical stretch].

Objective: To investigate the effect of emodin lipid nano-microbubble on MAPK signaling pathway and the level of inflammation cytokine in AT-II cells induced by mechanical stretch and its mechanism.

Methods: Emodin nanofibers, prepared by electrospinning with lecithin and PVP as carrier, were put into a seal bottle full of perfluoropropane, after they mixed with water and turned into self-assembled nano-microbubble. AT-II cells, separated and purified from primary rat AT-II cells, suffered 20% mechanical stretch for 4, 8, 16, 24, 48 h, and its protein expressions of p-P38/P38, p-ERK/ERK, p-JNK/JNK and the inflammation cytokine release levels of TNF-alpha, IL-1beta, IL-6 were detected by Western-Blot and ELISA.

[Effects of cyclic stretch on expression of cytokines and intercellular adhesion molecule-1 in human pulmonary artery endothelial cell].

Objective: To observe the effects of cyclic stretch on expression of cytokines and adhesion molecules in human pulmonary artery endothelial cells (HPAECs), herein to provide a theoretical basis to ventilator-induced lung injury (VILI).

Methods: HPAECs were subjected to cyclic stretch by the Flexcell FX-5000T system at 0.5 Hz of 10% or 20% elongation for 3, 6, 12, 24 hours respectively.

[Lipopolysaccharide induced expression of peroxiredoxin 1 in airway epithelial cell].

Objective: To investigate the effect of lipopolysaccharide (LPS) on expression of peroxiredoxin 1 (prdx1) in airway epithelial cells.

Methods: The airway epithelium cell line BEAS-2B was cultivated, and the cells were stimulated with 0, 1, and 10 mg/L of LPS for 12 hours and 24 hours, and then were harvested for prdx1 expression detection. The mRNA expression of prdx1 was detected by reverse transcription-polymerase chain reaction (RT-PCR).

Electrospun drug-loaded core-sheath PVP/zein nanofibers for biphasic drug release.

Core-sheath nanofibers prepared using coaxial electrospinning were investigated for providing biphasic drug release profiles. With ketoprofen (KET) as the model drug, polyvinylpyrrolidone and zein as the sheath polymer and core matrix, respectively, the coaxial process could be carried out smoothly and continuously without any clogging of the spinneret. Scanning electron microscopy and transmission electron microscopy observations demonstrated that the nanofibers were linear with homogeneous structure and had a clear core-sheath structure with an average diameter of 730 ± 190 nm, in which the sheath had a thickness of ca.

[Isolation and characterization of human alveolar type II cells and phenotypes maintaining study].

Objective: To establish a method of isolate, purify, primary culture and identify human alveolar type II cells (AT II ) in vitro, as well as its possible maintaining phenotype characteristics.

Methods: The marginal lung tissue was collected. AT II cells were isolated with trypsin and elastase, purified by a series of steps, such as, cell sieve filtration, differential adhesion, gradient separation and anti-CD14 beads separation.

[Gene transfer system mediated by PEI-cholesterol lipopolymer with lipid microbubbles].

The properties of polyethyleneimine-cholesterol cationic lipopolymer (PEI-Chol) as gene carries and its gene transfer efficiency in vitro with lipid microbubbles were presented in this paper. PEI-Chol lipopolymer was synthesized by linking cholesterol chloroformate to the amino groups of branched poly(ethyleneimine) (PEI) of 1 800. The structure and molecular weight of PEI-Chol were confirmed by IR, 1H NMR and MADI-TOF-MS (matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry), respectively.

[Preparation of self-emulsifying soft capsule and its pharmacokinetic in rats for epimedium flavonoids].

Objective: To study the formulation optimization of epimedium flavonoids self-emulsifying drug delivery system and evaluate its effect in vitro and in vivo.

Methods: Based on the degree of emulsification and emulsifying time, the formulation optimization (i.e.

[Study on the natural ligand(s) of triggering receptor expressed on myeloid cell-1 on bacteria cell wall].

Objective: To look for the natural ligand(s) of human triggering receptor expressed on myeloid cell-1 (TREM-1), in order to provide the theoretical basis for elucidation of the pathogenesis of sepsis.

Methods: Neutrophils and monocytes isolated from human peripheral blood were treated with heat-inactivated Staphylococcus aureus, Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus L-form or Pseudomonas aeruginosa L-form respectively for 24 hours. The cell wall was extracted from Staphylococcus aureus, Pseudomonas aeruginosa and Mycobacterium tuberculosis by ultrasound.

Evaluation of the Chinese versions of the Minnesota nicotine withdrawal scale and the questionnaire on smoking urges-brief.

Objectives: To evaluate the reliability and validity of the Chinese versions of Minnesota Nicotine Withdrawal Scale and Questionnaire on Smoking Urges-Brief (MNWS-C and QSU-Brief-C) in Chinese smokers.

Design And Methods: MNWS and QSU-Brief were translated into Chinese version through 10 steps. The reliability and validity of Chinese versions of the two scales were evaluated based on the data collected from 354 subjects.

[Effects of Tankejing dry powder inhaler on the inflammatory damage in the lungs of mice infected by FM1].

Objective: To study the effect of Tankejing Dry Powder inhaler on inflammatory in lung injury of mice infected by influenza virus (FM1).

Methods: Model mice infected by influenza virus FM1 were randomly divided into six groups: the nomal group, model group, low dose, medium dose and high dose of Tankejing Dry Powder inhaler group,and Ribavirin group. The following indices including the change of NF-kappaB in lung tissue at 24 h, the changes of TNF-alpha, IL-1beta, IL-6 in lung tissue, the lung index and the death rate were observed.

[Anti-viral effects of Tankejing preparations against influenza virus A].

Objective: To investigate the anti-viral effects of Tankejing preparations (including solutions, dry power inhalation and powder) against influenza virus A in vitro and the relationship between its anti-viral effect and preparations.

Methods: The inhibitive effect of different drug-added ways of Tankejing extracts against human influenza virus A (H3N2) in vitro were assayed by crystallized purple staining method with ribavirin as positive reference drug. Then we compared the anti-viral actions of different kinds of Tankejing preparations.

Losartan prevents sepsis-induced acute lung injury and decreases activation of nuclear factor kappaB and mitogen-activated protein kinases.

Lack of specific and efficient therapy leads to the high mortality rate of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). Losartan is a potent pharmaceutical drug for ALI/ARDS. However, the protective effects and mechanisms of losartan remain incompletely known.

[Expression and significance of severe acute respiratory syndrome associated coronavirus (SARS-CoV)-X4 protein in lungs of SARS patients].

Objective: To investigate the significance of severe acute respiratory syndrome associated coronavirus (SARS-CoV)-X4 protein expression in lungs of patients with SARS.

Methods: Pathological features of the lungs from 4 SARS patients were examined and the expression of SARS-CoV-X4 protein in the lungs was evaluated with immunohistochemical staining using specific antibodies against protein X4.

Results: Microscopically, all lungs from 4 cases showed edema, erythrocyte and fibrin exudates in the alveoli, hyperplasia of alveolar epithelium, necrosis, hyaline membrane formation and fibroblast foci.

Longitudinal profile of antibodies against SARS-coronavirus in SARS patients and their clinical significance.

Background: Severe acute respiratory syndrome (SARS) is a newly discovered disease caused by a novel coronavirus. The present study studied the longitudinal profile of antibodies against SARS-coronavirus (SARS-CoV) in SARS patients and evaluated the clinical significance of these antibodies.

Methods: Two methods, ELISA and indirect immunofluorescent assay, were used for the detection of the anti-SARS-CoV IgG and IgM in 335 serial sera from 98 SARS patients.

Evaluation by indirect immunofluorescent assay and enzyme linked immunosorbent assay of the dynamic changes of serum antibody responses against severe acute respiratory syndrome coronavirus.

Background: Severe acute respiratory syndrome coronavirus (SARS-CoV) is a newly emerging virus that gives rise to SARS patients with high rates of infectivity and fatality. To study the humoral immune responses to SARS-CoV, the authors evaluated IgG and IgM specific antibodies in patients' sera.

Methods: Two methods, enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescent assay (IFA), were used to detect specific serum IgG and IgM against SARS-CoV in 98 SARS patients and 250 controls consisting of patients with pneumonia, health-care professionals and healthy subjects.

[Protective effect of specific antibody in serum of convalescent patient with SARS].

Objective: To investigate inhibitory effect of serum severe acute respiratory syndrome (SARS) -specific antibodies from convalescent patients after half an year of onset on SARS-CoV-mediated cytopathic response.

Methods: SARS-CoV immunoglobulin G (IgG) antibody was determined by enzyme linked immunoadsorbent assay (ELISA). Twelve serum samples from convalescent patients, diluted by 1:8 with maintenance medium, were mixed with the three dilution supernatants of SARS-CoV.

[Effects of epimedium total flavonoids phytosomes on preventing and treating bone-loss of ovariectomized rats].

Objective: To observe the effects of Epimedium total Flavonoids Phytosomes on preventing and treating bone-loss of the castrate osteoporosis rat model.

Method: The osteoporosis model was established with 4-month-odl panther's rats, their ovaries on both sides castrated. Dual energy X-ray scanning was used to determine the bone density, and immunity and ELASA were used to assay concentration of estradiol and IL-6 in serum respectively, then determine their effect.