Heterologous expression, enzymatic properties, product analysis and molecular docking of assimilative nitrite reductase (NiR) in Bacillus velezensis GXMZU-B1 derived from mariculture.

High concentrations of nitrite stress aquatic animals, leading to significant fish and shrimp deaths as well as environmental pollution. Reducing nitrite levels in high-density aquaculture is crucial for both aquaculture safety and environmental protection. Nitrite reductase (NiR) can rapidly reduce nitrite in water, offering potential applications in aquaculture and water treatment.

Guselkumab in patients with moderately to severely active ulcerative colitis (QUASAR): phase 3 double-blind, randomised, placebo-controlled induction and maintenance studies.

Background: Interleukin-23 inhibition is effective in treating ulcerative colitis. Guselkumab is a dual-acting, human IgG1, interleukin-23p19 subunit inhibitor that potently neutralises interleukin-23 and can bind to CD64. We aimed to evaluate the efficacy and safety of guselkumab as induction and maintenance therapy in patients with ulcerative colitis.

Factors associated with preterm birth and mother-to-child transmission in HIV-positive pregnant women in Henan, China, 2016-2022: a retrospective cohort study.

Introduction: HIV can greatly impact the quality of life of pregnant women and may cause adverse pregnancy outcomes, such as preterm birth (PB) and mother-to-child transmission (MTCT). The purpose of this study was to analyse the influencing factors of PB and MTCT in HIV-positive pregnant women.

Methods: HIV-positive pregnant women in Henan Province between January 2016 and December 2022 were selected for the study.

Current status and future prospects of combined immunotherapy and epidermal growth factor receptor inhibitors in head and neck squamous cell carcinoma.

Head and neck squamous cell carcinoma (HNSCC) is a malignancy with a poor prognosis, and the majority of patients with HNSCC are diagnosed at later stages owing to its hidden anatomical location and atypical clinical symptoms. It is notably prone to recurrence and metastasis. The traditional treatments include surgery, radiotherapy, chemotherapy, and targeted therapy.

Precise targeting of lipid metabolism in the era of immuno-oncology and the latest advances in nano-based drug delivery systems for cancer therapy.

Over the past decade, research has increasingly identified unique dysregulations in lipid metabolism within the tumor microenvironment (TME). Lipids, diverse biomolecules, not only constitute biological membranes but also function as signaling molecules and energy sources. Enhanced synthesis or uptake of lipids in the TME significantly promotes tumorigenesis and proliferation.

Simulation of nitrate nitrogen concentrations and DOM characteristics in groundwater from Southwest China's Karst Wetlands using an improved GMS model.

Existing models often face limitations in the understanding and prediction of nitrate nitrogen (NO-N) concentrations in karst groundwater. In this study, to tackle this issue, a Gaussian function model was coupled with the Groundwater Modeling System (GMS) to simulate NO-N concentration changes in the southwest karst wetland of China. Additionally, fluorescence spectroscopy was employed to measure dissolved organic matter (DOM) components in the groundwater, providing insights into their variation and influence on NO-N dynamics.

Assessment of clinical efficacy and safety of ICL implantation in patients with relatively shallow anterior chamber depth in early and midterm postoperative time.

Objective: To investigate the clinical effect and safety of Implantable Collamer Lens (ICL) implantation in myopic patients with relatively shallow anterior chamber depth (ACD).

Methods: Retrospective analysis, comparative, non-interventional case series. Patients with myopia who underwent ICL implantation were included in the study and were categorized into two groups: one with a relatively shallow ACD (2.

Synthesis of 2MP-CuNPs Fluorescent Probes and Their Application in Tetracycline Detection.

A fluorescent probe composed of 2-mercaptopyridine-copper nanoparticles (2MP-CuNPs) was synthesized through a hydrothermal method utilizing CuCl and 2-mercaptopyridine (2MP). The experimental results indicate that the 2MP-CuNPs probe exhibited an excellent fluorescence emission peak at 525 nm with an excitation wavelength of 200 nm. Furthermore, this emission peak was accompanied by a substantial Stokes shift of 325 nm, which effectively minimized the overlap between the excitation and emission spectra, thereby enhancing detection sensitivity.

Outcome and toxicities of postmastectomy radiotherapy with integral cervicothoracic thermoplastic mask.

Background: Appropriate immobilization setup for postmastectomy radiotherapy may help to improve tumor control and to reduce radiation-related toxicities. This study aims at retrospectively evaluate the outcome and toxicities of postmastectomy radiotherapy (PMRT) with a novel integral cervicothoracic thermoplastic mask strategy.

Methods: Breast cancer patients were treated with modified radical mastectomy and PMRT.

Live-Cell RNA Imaging with a DNA-Functionalized Metal-Organic Framework-Based Fluorescent Probe.

Live-cell fluorescence imaging of tumor-associated miRNAs is significant for understanding the cancer onset and progression and the diagnosis and prognosis of clinical diseases. In this protocol, we describe the construction of a DNA-functionalized metal-organic framework-based fluorescent probe and demonstrate that the probe can be used to detect miRNA in vitro and live-cell imaging. The formed DNA-MOF probe can specifically target miRNA with high sensitivity and specificity.

Pressure Injuries and the Waterlow Subscales in the Intensive Care Unit: A Multicentre Study.

Background: Pressure injuries (PIs) impose a significant burden on patients in the intensive care unit (ICU) and the healthcare system. Assessing the risk of developing PIs is crucial for prevention. However, it is unclear whether all subscales of the Waterlow scale can be used to assess PIs risk in ICU.

Comparative efficacy and safety of Chinese patent medicines as an adjunctive therapy for diabetic peripheral neuropathy: systematic review and network meta-analysis of randomized controlled trials.

Context: Diabetic peripheral neuropathy (DPN) is the most common complication of diabetes mellitus. Chinese patent medicines (CPMs) are widely used in clinical practice to treat DPN.

Objective: This study aims to summarize the latest evidence on the harms and benefits of CPMs as adjunctive therapy for DPN.

On propagation characteristics of ultrasonic guided waves in layered fluid-saturated porous media using spectral method.

Fluid-saturated porous media plays an increasingly important role in emerging fields such as lithium batteries and artificial bones. Accurately solving the governing equations of guided wave is the key to the successful application of ultrasonic guided wave nondestructive testing technology in fluid-saturated porous media. This paper derives the Lamb wave equation in layered fluid-saturated porous materials based on Biot theory and proposes the spectral method suitable for solving complex wave equations.

NCU-04 relieves constipation and the depressive-like behaviors induced by loperamide in mice through the microbiome-gut-brain axis.

Constipation is a prevalent gastrointestinal condition that significantly affects patients' physical and mental well-being, yet current treatments often lack safety and efficacy. Emerging evidence highlights the critical role of the microbiota-gut-brain axis (MBGA) in managing constipation, paving the way for probiotics as an adjuvant treatment to improve constipation symptoms. In this study, we isolated a gut probiotic strain, NCU-04, and investigated its improvement effects on loperamide-induced constipation in mice.

Simultaneous determination of pesticides, mycotoxins and ferulic acid in Angelica sinensis by GC/LC-Q-TOF/MS.

In this study, a strong applicable method that could determine a total of 33 pesticides (54 compounds), 11 mycotoxins and functional components (ferulic acid) simultaneously in Angelica sinensis was developed. The compatibility of the sample pretreatment method for pesticides, mycotoxins, and functional components was improved by optimizing the acidity of extraction solvents, the sequence of ice bath and oscillation, the volumetric solution, and so on. The PRiME HLB SPE pretreatment method was chosen as the optimal one when comparing four pretreatment methods.

Antimicrobial mechanisms and antifungal activity of compounds generated by banana rhizosphere Gxun-2 against f. sp. .

Introduction: wilt of banana, also recognized as Panama disease, is caused by the soil-borne fungus f. sp. tropical race 4 (FOC TR4).

Plant lncRNA-miRNA Interaction Prediction Based on Counterfactual Heterogeneous Graph Attention Network.

Identifying interactions between long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) provides a new perspective for understanding regulatory relationships in plant life processes. Recently, computational methods based on graph neural networks (GNNs) have been widely employed to predict lncRNA-miRNA interactions (LMIs), which compensate for the inadequacy of biological experiments. However, the low-semantic and noise of graph limit the performance of existing GNN-based methods.

LN-439A, a novel BAP1 inhibitor, suppresses the growth of basal-like breast cancer by degrading KLF5.

Basal-like breast cancer (BLBC) is the most malignant subtype of breast cancer because of its aggressive clinical behaviour and lack of effective targeted agents. Krüppel-like factor 5 (KLF5) is an oncogenic transcription factor that is highly expressed in BLBC. The deubiquitinase (DUB) BRCA1-associated protein 1 (BAP1) stabilizes KLF5 and promotes BLBC growth and metastasis.