Adipose mesenchymal stem cells-derived exosomes attenuated hyperoxia-induced lung injury in neonatal rats via inhibiting the NF-κB signaling pathway.

Objective: Bronchopulmonary dysplasia (BPD) is the most common chronic morbidity in extremely preterm infants. Mesenchymal stem cells-derived exosomes (MSC-Exos) therapies have shown prospects in animal models of BPD. Our study aimed to evaluate the effect of adipose mesenchymal stem cells-derived exosomes (AMSC-Exos) on BPD and the role of the NF-κB signaling pathway in this process.

Engineering and finetuning expression of SerC for balanced metabolic flux in vitamin B production.

Vitamin B plays a crucial role in cellular metabolism and stress response, making it an essential component for growth in all known organisms. However, achieving efficient biosynthesis of vitamin B faces the challenge of maintaining a balanced distribution of metabolic flux between growth and production. In this study, our focus is on addressing this challenge through the engineering of phosphoserine aminotransferase (SerC) to resolve its redundancy and promiscuity.

Characterization of a Riboflavin-Producing Mutant of Isolated by Droplet-Based Microfluidics Screening.

is one of the commonly used industrial strains for riboflavin production. High-throughput screening is useful in biotechnology, but there are still an insufficient number of articles focusing on improving the riboflavin production of by this powerful tool. With droplet-based microfluidics technology, single cells can be encapsulated in droplets.

Case Report: Chinese female patients with a heterozygous pathogenic gene variant c.898C>T and distal 22q11.2 microdeletion.

Coffin-Lowry syndrome (CLS) [OMIM#303600] is a rare X-linked dominant syndrome. CLS is caused by highly heterogeneous loss-of-function mutations in the gene (OMIM*300,075). CLS is characterized by intellectual disability (ID), short stature, tapered fingers, characteristic facial features, and progressive skeletal changes.

Low-Dose Vitamin D Protects Hyperoxia-Induced Bronchopulmonary Dysplasia by Inhibiting Neutrophil Extracellular Traps.

As bronchopulmonary dysplasia (BPD) can lead to considerable mortality and morbidity, this disease is the focus of attention in neonatology. Vitamin D (VD), which has anti-inflammatory properties and promotes lung growth, may have a therapeutic effect on BPD. The overexpression of neutrophil extracellular traps (NETs) has been demonstrated to be involved in the pathogenesis of BPD in our previous study.

Epidemiological Study of Influenza A, Influenza B, Enterovirus 71, and Coxsackievirus A16 Among Children in the Central Region of Zhejiang Province, China.

Influenza A virus (IAV), influenza B virus (IBV), enterovirus 71 (EV71), and coxsackievirus A16 (CVA16) are common pathogens for viral infection in children. In order to investigate the epidemiology of these four viral infections in the central region of Zhejiang province, China, 10,638 respiratory secretion samples previously tested for IAV and IBV, and 6427 whole blood samples previously tested for EV71 and CVA16 detection were analyzed retrospectively. The present data shows that viral infections with these four viruses featured with distinct seasonal patterns.

The substitution of a single amino acid with its enantiomer for control over the adjuvant activity of self-assembling peptides.

The substitution of a single amino acid with its enantiomer may lead to variations in self-assembled nanostructures and biological functions. In this study, we reported three novel heterochiral peptide hydrogels, Nap-GFFY (gel-1), Nap-GFFY (gel-2) and Nap-GFFY (gel-3), from Nap-GFFY the substitution of a single amino acid with its enantiomer. We found that the resulting hydrogels possessed diverse self-assembly behaviors and adjuvant activities.

DABCO-mediated [3+3] annulation of para-quinamines: access to functionalized 1,2,4-triazinone derivatives.

An unprecedented [3+3]-annulation of p-quinamines and nitrile imines was developed, affording a series of 1,2,4-triazinone derivatives in moderate to excellent yields with excellent diastereoselectivity. This cycloaddition expands the scope of p-quinamines to the construction of six-membered nitrogen-containing heterocyclic compounds.

Phosphine-Catalyzed Domino [3 + 3] Cyclization of para-Quinamines with Morita-Baylis-Hillman Carbonates: Access to Hydroquinoline Derivatives.

A phosphine-catalyzed [3 + 3] cyclization strategy between para-quinamines and HCHO Morita-Baylis-Hillman (MBH) carbonates was discovered, delivering a series of highly functionalized hydroquinoline derivatives in moderate to good yields and excellent diastereoselectivity. Moreover, mechanistic insights, gram-scale experiments, and synthetic manipulations of the products were also discussed.

Sequential Phosphine-Catalyzed [4 + 2] Annulation of β'-Acetoxy Allenoates: Enantioselective Synthesis of 3-Ethynyl-Substituted Tetrahydroquinolines.

The first enantioselective sequential phosphine-catalyzed (SPC as abbreviation) mode for the formation of tetrahydroquinolines with an ethynyl-substituted all-carbon quaternary stereogenic center is reported. In this SPC process, a novel [4 + 2] annulation process was devised employing α-substituted allenoates as C2 synthons (α-β', 1,2-dipole) for the first time. 3-Ethynyl-substituted tetrahydroquinolines were readily prepared in good yields and high enantioselectivities.

Effect of the Gene Knock-out and Gene Knock-in on Mannitol Production in .

can be used to produce mannitol by fermentation, but the mannitol productivity is not high. Therefore, in this study modify the chromosome of by genetic methods to obtain high-yield strains of mannitol production. In this study, gene knock-out strains and gene knock-in strains were constructed by a two-step homologous recombination method.

DBU-Catalyzed Desymmetrization of Cyclohexadienones: Access to Vicinal Diamine-Containing Heterocycles.

A DBU-catalyzed desymmetrization strategy between cyclohexadienones and isocyanates was discovered, affording a series of vicinal diamine-containing heterocycle derivatives in moderate to good yields and excellent diastereoselectivity under mild conditions. Furthermore, this reaction could be performed on a 10 g scale using 1.0 mol % of catalyst loading.

Ni-Co layered double hydroxide on carbon nanorods and graphene nanoribbons derived from MOFs for supercapacitors.

In this study, carbon nanorods (CNR) and graphene nanoribbons (GNR) derived from metal-organic frameworks (MOFs) were first prepared by solvothermal method. Then, Ni-Co layered double hydroxide (LDH)/CNR and LDH/GNR composite materials for supercapacitors were synthesized using a facile co-precipitation method. With the help of GNR, the Ni-Co LDH/GNR composite material showed great specific capacity (1765 F g-1), rate performance (68% capacity retention when current density increased from 1 to 20 A g-1) and cycling stability (83% capacity retention after 2000 charge-discharge cycles at 5 A g-1).

Phosphine-catalyzed intramolecular Rauhut-Currier reaction: enantioselective synthesis of hydro-2H-indole derivatives.

A highly enantioselective intramolecular Rauhut-Currier reaction catalyzed by a multifunctional chiral aminophosphine catalyst was reported. A series of hydro-2H-indole derivatives that bear an all-carbon quaternary center were obtained in high yields (up to 94%), and excellent diastereo- and enantioselectivities (up to >20 : 1 dr and >99% ee). And this reaction could be performed on a gram scale using 2 mol% catalyst loading.

Assessment of prognostic scores of brain metastases from lung adenocarcinoma with EGFR mutations.

The aim of this study was to analyze prognostic factors and evaluate the value of four prognostic scores including RPA, DS-GPA BS-BM, GGS for the EGFR mutant BM patients from lung adenocarcinoma treated with EGFR-TKI. Data of NSCLC were retrospectively reviewed from August 2010 to June 2015 using the medical database of Shanxi Provincial Cancer Hospital. Patients with BM from lung adenocarcinoma with mutant EGFR treated by EGFR-TKI or a combination of EGFR-TKI and WBRT were included.

A phosphine mediated sequential annulation process of 2-tosylaminochalcones with MBH carbonates to construct functionalized aza-benzobicyclo[4.3.0] derivatives.

A novel phosphine mediated sequential annulation process to construct functionalized aza-benzobicyclo[4.3.0] derivatives has been developed involving a one-pot sequential catalytic and stoichiometric process, which generates a series of benzobicyclo[4.

[3 + 2]-Annulation of Prop-2-ynylsulfonium Salts: Access to Hydroindol-5-ones Containing a Methylthio Group.

An unprecedented [3 + 2]-annulation of prop-2-ynylsulfonium salts and p-quinamines was developed, affording a series of hydroindol-5-ones with a methylthio group in moderate to good yields under mild conditions. In this reaction, the prop-2-ynylsulfonium salt acts as a novel C synthon and sulfide does not serve as a leaving group, which provides facile access to organosulfur compouds.

Bifunctional-Phosphine-Catalyzed Sequential Annulations of Allenoates and Ketimines: Construction of Functionalized Poly-heterocycle Rings.

A highly stereoselective sequential annulation reaction between γ-substituted allenoates and ketimines was reported. By using bifunctional N-acyl aminophosphine catalysts, poly-heterocycle rings were obtained with high stereocontrol in good to excellent yields. The desired products have four contiguous stereogenic centers (one quaternary and three tertiary carbon centers), and only one isomer was obtained in all reactions.

The role of ¹⁸F-FDG uptake features in the differential diagnosis of solitary pulmonary lesions with PET/CT.

Background: The aim of this study is to evaluate the value of (18)F-FDG uptake features in the diagnosis of solitary pulmonary lesions.

Methods: One hundred thirty-nine patients with solitary pulmonary lesions were divided into full uptake, circular uptake, multi-focus uptake, mild uptake, and no-uptake groups according to the uptake features of (18)F-FDG in solitary pulmonary lesions. The incidence of benign and malignant lesions and the false-positive and false-negative rates in each group were analyzed.

Early and late Iron supplementation for low birth weight infants: a meta-analysis.

Background: Iron deficiency in infancy is associated with a range of clinical and developmentally important issues. Currently, it is unclear what is the optimal timing to administer prophylactic enteral iron supplementation in preterm and very low birth weight infants. The objective of this meta-analysis was to evaluate early compared with late iron supplementation in low birth weight infants.

Risk factors for recurrent respiratory infections in preschool children in china.

Objective: To identify and compare risk factors for recurrent respiratory infections in preschool children between resident and floating population in Yiwu, China.

Methods: Investigations was conducted in resident and floating population in Yiwu city, Zhejiang province. A structured questionnaire was used to collect information on influence factors.

Associations of IL-2 and IL-4 Expression and Polymorphisms With the Risks of Mycoplasma pneumoniae Infection and Asthma in Children.

Introduction: Asthma is an inflammatory disorder of the airways and the symptoms of asthma could be exacerbated by Mycoplasma pneumoniae infection. Interleukin-2 and interleukin-4 have been implicated in immune and inflammatory reactions. We examined the associations of IL2 and IL4 polymorphisms and expression with the risks of asthma and M.

Comparison of risk factors for recurrent respiratory infections between urban and rural preschool children in Yiwu, China.

Background: Many studies have shown an association between the risk of increased recurrent respiratory infections and socioeconomic and fostering factors, but often only a few risk factors have been studied. This study aimed to identify and compare such factors between urban and rural preschool children.

Methods: Case control studies were conducted in Yiwu urban and rural areas respectively in Zhejiang Province.