A small-scale silica gel column chromatography method for separating carbazole compounds from highly mature crude oil.

The concentration of carbazoles in highly mature crude oil is quite low, making it challenging to separate carbazole compounds for the gas chromatography-mass spectrometry (GC-MS) detection. This study presents a small-scale column chromatography method for separating carbazoles from highly mature crude oil using silica gel as a solid phase adsorbent and a Pasteur pipette as a separation device. The carbazole-rich crude oil from the Pearl River Mouth Basin was selected to explore the impact of reagent polarity and injection mode on the separation of carbazoles.

[Secondary Alterations Influence on Fluorescence Color and Spectral Parameters of Crude Oil and Oil Inclusion].

Based on oil samples and rock samples in several petroliferous basins, this paper studied that secondary alterations influence on fluorescence color and spectral parameters of crude oil and oil inclusions by using microscopic fluorescent spectral and geochemical parameters. The results demonstrated that secondary alterations influence on spectral parameters by changing the ratio of saturated hydrocarbon and aromatic hydrocarbon (saturation/aromatics). Biodegradation and water washing lead to redshift of fluorescence parameters while evaporative fractionation and oil cracking lead to blueshift.