Regulation Roles of Juvenile Hormone Epoxide Hydrolase Gene 2 in the Female River Prawn Reproductive Process.

In this study, we investigated the regulatory roles of the () gene in the reproductive process of female . Its total cDNA length was 1848 bp, encoding for 460 amino acids. It contained conserved domains typical of epoxide hydrolases, such as the Abhydrolase family domain, the EHN epoxide hydrolase superfamily domain, and the "WWG" and "HGWP" motifs.

Genome-wide association and transcriptomic analysis and the identification of growth-related genes in Macrobrachium nipponense.

Macrobrachium nipponense is a commercially important freshwater species of prawn that is widely distributed across Asian countries. In order to investigate the molecular mechanisms of growth in M. nipponense, and to provide a foundation for molecular breeding, we used genome-wide association analysis (GWAS) and transcriptomic analysis to screen polymorphisms and genes related to growth traits.

A study on the functional role of the DHCR24 gene in gonadal differentiation and development of Macrobrachium nipponense.

Sex differentiation in crustaceans is a complex process influenced by various factors, including the androgenic gland and sex-related genes. This study characterized the role of the Mn-DHCR24 gene in the oriental river prawn (Macrobrachium nipponense). We used bioinformatics to analyze sequence features and phylogenetic relationships of a single Mn-DHCR24 gene.

Transcriptomic Analysis of the Hepatopancreas in the Sex-Related Size Differences of .

, a commercially popular crustacean species within the Chinese context, is recognized for its exceptional nutritional composition and palatability. There are significant differences in growth between male and female . Herein, transcriptomics was used to determine the hepatopancreas transcriptome differences between sex-related size differences in .

Identification of the effects of alkalinity exposure on the gills of oriental river prawns, Macrobrachium nipponense.

Macrobrachium nipponense is an important commercial freshwater species in China. However, the ability of alkali tolerance of M. nipponense is insufficient to culture in the major saline-alkali water source in China.

Functional Study of the Role of the Gene in the Ovarian Development of .

() is a gene encoding an enzyme related to the last step of juvenile hormone biosynthesis. cDNA has a total length of 1695 bp and an open reading frame (ORF) length of 1482 bp, encoding 493 amino acids. Sequence analysis showed that its amino acid sequence has a PPGP hinge, an FGCG structural domain, and other structural domains specific to the P450 family of enzymes.

Studies on the Relationships between Growth and Gonad Development during First Sexual Maturation of and Associated SNPs Screening.

In this study, we used full-sib families to investigate the association between growth and gonad development during first sexual maturation of . We found that male GSI was significantly negatively correlated with growth traits ( < 0.01) and there were no significant correlations between female GSI (Gonadosomatic index) and growth traits ( > 0.

NPC Intracellular Cholesterol Transporter 1 Regulates Ovarian Maturation and Molting in Female .

NPC intracellular cholesterol transporter 1 (NPC1) plays an important role in sterol metabolism and transport processes and has been studied in many vertebrates and some insects, but rarely in crustaceans. In this study, we characterized NPC1 from () and evaluated its functions. Its total cDNA length was 4283 bp, encoding for 1344 amino acids.

Identifying Relationships between Single Nucleotide Polymorphisms and Hypoxia Tolerance and Growth Traits in .

Investigating hypoxia tolerance and growth trait single nucleotide polymorphisms (SNPs) in is conducive to cultivating prawns with hypoxia tolerance and good growth characteristics. The glutathione S-transferase-2 gene () has been shown to regulate hypoxia responses in . In this study, we identified a single SNP in , and analyzed its regulatory relationship with hypoxia tolerance and growth.

Identification of Candidate Male-Reproduction-Related Genes from the Testis and Androgenic Gland of , Regulated by through Transcriptome Profiling Analysis.

The previous publication identified that pyruvate dehydrogenase E1 () positively regulated the process of male reproduction in through affecting the expressions of insulin-like androgenic gland hormone. The present study aimed to identify the potential male-reproduction-related genes that were regulated by through performing the transcriptome profiling analysis in the testis and androgenic gland after the knockdown of the expressions of by the injection of . Both RNA-Seq and qPCR analysis identified the significant decreases in expressions in the testis and androgenic gland in injected prawns compared to those in -injected prawns, indicating the efficiency of in the present study.

Role of in Sex Hormone Regulation and Gonadal Development in the Oriental River Prawn, .

This study investigates the role of lysosomal acid lipase in sex hormone regulation and gonadal development in . The full-length cDNA was cloned, and its expression patterns were analyzed using quantitative real-time PCR (qPCR) in various tissues and developmental stages. Higher expression levels were observed in the hepatopancreas, cerebral ganglion, and testes, indicating the potential involvement of in sex differentiation and gonadal development.

Effects of Alkalinity Exposure on Antioxidant Status, Metabolic Function, and Immune Response in the Hepatopancreas of .

The oriental river prawn is an important freshwater economic species in China, producing huge economic benefits. However, shows lower alkali tolerance than fish species, thus genetic selection is urgently needed in order to improve alkali tolerance in this species. In the present study, the effects of alkalinity exposure on the hepatopancreas of were measured under the alkali concentrations of 0 (control), 4, 8, and 12 mmol/L with the exposure time of 96 h through histological observations, measurement of antioxidant enzymes, metabolic profiling analysis, and transcriptome profiling analysis.

Identification of genes regulated by 20-Hydroxyecdysone in Macrobrachium nipponense using comparative transcriptomic analysis.

Background: Macrobrachium nipponense is a freshwater prawn of economic importance in China. Its reproductive molt is crucial for seedling rearing and directly impacts the industry's economic efficiency. 20-hydroxyecdysone (20E) controls various physiological behaviors in crustaceans, among which is the initiation of molt.

Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in : Insights into Reproduction, Growth, and Sex Differentiation.

This study investigated the potential to use double-stranded RNA insulin-like androgenic gland hormone () to induce sex reversal in and identified the molecular mechanisms underlying crustacean reproduction and sex differentiation. The study aimed to determine whether could induce sex reversal in PL30-male during a critical period. The sex-related genes were selected by performing the gonadal transcriptome analysis of normal male (dsM), normal female (dsFM), neo-female sex-reversed individuals (dsRM), and unreversed males (dsNRM).

Identification of Male Sex-Related Genes Regulated by in Based on Transcriptome Analysis after an RNAi Knockdown.

The oriental river prawn () is a commercially important species in Asia. A previous study showed that the succinate dehydrogenase complex iron sulfur subunit B () gene participates in testes development in this species through its effect on the expression of the insulin-like androgenic gland hormone gene. This study knocked-down the genes in using RNAi.

Correction: Cai et al. Sex Reversal Induced by Dietary Supplementation with 17α-Methyltestosterone during the Critical Period of Sex Differentiation in Oriental River Prawn (). 2023, , 1369.

In the original publication [...

Has an Inhibitory Effect on Ovarian Reproduction in .

is an exoribonuclease that degrades mRNA in the cytoplasm along the 5'-3' direction. A previous study indicated that it may be involved in the reproduction of . Quantitative real-time PCR was used to detect the spatiotemporal expression pattern of .

Deciphering Molecular Mechanisms Governing the Reproductive Molt of : A Transcriptome Analysis of Ovaries across Various Molting Stages.

The relationship between molting and reproduction has received more attention in economically important crustacean decapods. Molting and reproduction are synergistic events in , but the molecular regulatory mechanisms behind them are unclear. In the current study, we performed Illumina sequencing for the ovaries of during the molt cycle (pre-molting, Prm; mid-molting, Mm; and post-molting, Pom).

Dual roles of CYP302A1 in regulating ovarian maturation and molting in Macrobrachium nipponense.

CYP302A1 is a member of the Halloween genes in the cytochrome P450 supergene family, which play an important regulatory role in the synthesis of 20-hydroxyecdysone (20E) in crustaceans and insects. In this study, we found that the Mn-CYP302A1sequence included typical CYP450 conserved domains. Phylogenic showed that it is closely related to crustaceans and insects.

17β-Estradiol Induced Sex Reversal and Gonadal Transcriptome Analysis in the Oriental River Prawn (): Mechanisms, Pathways, and Potential Harm.

Sex reversal induced by 17β-estradiol (E) has shown the potential possibility for monoculture technology development. The present study aimed to determine whether dietary supplementation with different concentrations of E could induce sex reversal in , and select the sex-related genes by performing the gonadal transcriptome analysis of normal male (M), normal female (FM), sex-reversed male prawns (RM), and unreversed male prawns (NRM). Histology, transcriptome analysis, and qPCR were performed to compare differences in gonad development, key metabolic pathways, and genes.

RNAi Analysis of Potential Functions of Cyclin B3 in Reproduction of Male Oriental River Prawns ().

Cyclin B3 () is involved in the metabolic pathway of the cell cycle, playing essential roles in the regulation of cell proliferation and mitosis. is also predicted to be involved in the reproduction of male oriental river prawns (). In this study, the potential functions of in were investigated using quantitative real-time PCR, RNA interference, and histological observations.

Function Analysis of Cholesterol 7-Desaturase in Ovarian Maturation and Molting in : Providing Evidence for Reproductive Molting Progress.

The Cholesterol 7-desaturase gene plays an important role in insect ecdysone synthesis, but its role in ovarian development has not been reported. In this study, characteristics and the phylogenetic relationship of Cholesterol 7-desaturase were identified by bioinformatics. qPCR showed that the gene was highly expressed in the ovary, which was much higher than that in other tissues, and the expression level of reached the highest level at the third stage of the ovarian development stage (O-III).

Sex Reversal Induced by Dietary Supplementation with 17α-Methyltestosterone during the Critical Period of Sex Differentiation in Oriental River Prawn ().

The steroid 17α-methyltestosterone (MT) inhibits ovarian function and is often used to induce sex reversal artificially in vertebrates. In the present study, different concentrations of MT were added as dietary supplementation, and the effects on sex ratio, growth, and gonadal development were examined. After 40 days, the sex ratio (male:female) in each group increased at different degrees with 50 (1.

Genetic diversity and population structure of wild Macrobrachium nipponense populations across China: Implication for population management.

Background: Macrobrachium nipponense, is an important economic indigenous prawn and is widely distributed in China. However, most these genetic structure analysis researches were focused on a certain water area, systematic comparative studies on genetic structure of M. nipponense across China are not yet available.