The Characterization of a Novel D-allulose 3-Epimerase from and Its Application in D-allulose Production.

D-allulose is a natural rare sugar with important physiological properties that is used in food, health care items, and even the pharmaceutical industry. In the current study, a novel D-allulose 3-epimerase gene (Bp-DAE) from the probiotic strain was discovered for the production and characterization of an enzyme known as Bp-DAE that can epimerize D-fructose into D-allulose. Bp-DAE was strictly dependent on metals (Mn and Co), and the addition of 1 mM of Mn could enhance the half-life of Bp-DAE at 55 °C from 60 to 180 min.