Simultaneous detection of norepinephrine and 5-hydroxytryptophan using poly-alizarin/multi-walled carbon nanotubes-graphene modified carbon fiber microelectrode array sensor.

Multi-walled carbon nanotubes, graphene and alizarin polymer composites coated carbon fiber microelectrode array sensor (p-AZ/MWCNT-GR/CFMEA) was constructed and used for the simultaneous detection of norepinephrine (NE) and 5-hydroxytryptophan (5-HT). The morphology and structural characteristics of sensor are characterized using scanning electron microscopy, energy dispersive spectroscopy and X-ray diffraction. Its electrochemical behavior has been studied with cyclic voltammetry and electrochemical impedance spectroscopy.

Carbon fiber microelectrode array loaded with the diazonium salt-single-walled carbon nanotubes composites for the simultaneous monitoring of dopamine and serotonin in vivo.

Carbon fiber microelectrode arrays based on diazonium salt and single-walled carbon nanotubes composites (DS-SWCNT/CFMEA) have been fabricated, and it developed for the simultaneous monitoring of dopamine (DA) and serotonin (5-HT) with differential pulse voltammary (DPV). The diazonium salt can improve the water-solubility of single-walled carbon nanotubes and show good selectivity to DA, thus DS-SWCNT/CFMEA exhibits enhanced electrocatalytic activity for the oxidation of DA and 5-HT, and well antifouling ability to the other biomolecules. Moreover, DS-SWCNT/CFMEA shows the wider liner range, and the good performance of precision, reproducibility and biocompatibility.

Electrochemical dual signal sensing platform for the simultaneous determination of dopamine, uric acid and glucose based on copper and cerium bimetallic carbon nanocomposites.

A highly sensitive electrochemical sensor for the simultaneous dual signal determination of dopamine (DA), uric acid (UA) and glucose (Glu) has been obtained using nanocomposites based on the copper and cerium bimetallic nanoparticles and carbon nanomaterials of graphene and single-walled carbon nanotubes in the presence of Tween 20 (GR-SWCNT-Ce-Cu-Tween 20) modified glassy carbon electrode. The surface morphology of the nanocomposites was characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD), and the electrochemical behavior of the sensor was investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) with potassium ferricyanide as probe. In the coexistence system of DA, UA and Glu, three clear and well-isolated voltammetric peaks were obtained by CV and differential pulse voltammetry (DPV), and oxidation peak currents of DA and UA are positively correlated with their concentrations respectively, while the peak current of Glu is negatively correlated with its concentration.

A functional ratio fluorescence sensor platform based on the graphene/Mn-ZnS quantum dots loaded with molecularly imprinted polymer for selective and visual detection sinapic acid.

A functional ratio fluorescence sensor based on the molecularly imprinted polymer (MIP) coated double quantum dots (QDs) being composited of Mn-ZnS QDs and silica-coated graphene quantum dots (GQDs@SiO) had been established for the sensitive, selective and visual detection of sinapic acid (SA). MIPs@Mn-ZnS/GQDs@SiO was synthesized through a simple one-pot sol-gel reaction, and it exhibited two fluorescence emission peaks with yellow fluorescence of Mn-ZnS QDs at 580 nm and the blue fluorescence of GQDs at 445 nm. SA can selectively enhance the fluorescence of GQDs but quench the Mn-ZnS QDs fluorescence to the MIPs@Mn-ZnS/GQDs@SiO.

A synthetic Rev-independent bovine immunodeficiency virus-based packaging construct.

Replication competent lentivirus (RCL) has been the major safety concern associated with applications of lentivirus-based gene transfer systems for human gene therapy. Minimization and elimination of overlaps between the packaging and the transfer vector constructs are expected to reduce the potential to generate RCL. We previously developed second- and third-generation bovine immunodeficiency virus (BIV)-based gene transfer systems.

Anti-angiogenic role of angiostatin during corneal wound healing.

The purpose of this study is to determine whether angiostatin is involved in maintaining corneal avascularity after wounding. We generated polyclonal rabbit anti-mouse angiostatin antibodies directed against each of the five kringle domains, (K1-5) and anti-mouse plasmin B chain antibodies. Mouse corneas were immunostained with anti-K1 angiostatin antibody after excimer laser keratectomy.

Corneal neovascularization after excimer keratectomy wounds in matrilysin-deficient mice.

Purpose: Matrilysin, matrix metalloproteinase (MMP)-7, is upregulated in the corneal epithelium during wound healing after excimer keratectomy wounds. The purpose of this study was to determine the role of matrilysin in maintaining corneal avascularity during wound healing.

Methods: Matrilysin-deficient mice (n = 17) and their age-matched wild-type littermates (n = 18) were treated with 193 nm argon-fluoride excimer keratectomy (experiment I).