[Dynamic monitoring and analysis of the persisters emerging in Candida albicans biofilm].

Purpose: To investigate the mechanism and approach related to persisters emerging in Candida albicans biofilm by studying its dynamic characteristics.

Methods: The Candida albicans biofilms model in vitro were formed by individual yeast cells and hyphal compartments respectively. The numbers of progenitive fungal cells and persisters at different stages were counted and analyzed with SPSS11.

[The construction and expression of Saccharomyces albicans pACT1-GFP plasmids].

Objective: To construct strains containing green fluorescent protein (GFP) to study gene regulation in Saccharomyces albicans cells during the infection process.

Methods: pACT1-GFP was constructed, and Saccharomyces albicans CAI4 was transformed. The expression of GFP in yeast and hyphal compartments was observed with microscopy.