Platelet membrane camouflaged AIEgen-mediated photodynamic therapy improves the effectiveness of anti-PD-L1 immunotherapy in large-burden tumors.

Although immunotherapy has achieved recent clinical success in antitumor therapy, it is less effective for solid tumors with large burdens. To overcome this challenge, herein, we report a new strategy based on platelet membrane-camouflaged aggregation-induced emission (AIE) luminogen (Plt-M@P) combined with the anti-programmed death ligand 1 (anti-PD-L1) for tumoral photodynamic-immunotherapy. Plt-M@P is prepared by using poly lactic-co-glycolic acid (PLGA)/PF3-PPh complex as a nanocore, and then by co-extrusion with platelet membranes.

Subtropical estuarine carbon budget under various hydrologic extremes and implications on the lateral carbon exchange from tidal wetlands.

As coastal areas become more vulnerable to climatic impacts, the need for understanding estuarine carbon budgets with sufficient spatiotemporal resolution arises. Under various hydrologic extremes ranging from drought to hurricane-induced flooding, a mass balance model was constructed for carbon fluxes and their variabilities in four estuaries along the northwestern Gulf of Mexico (nwGOM) coast over a four-year period (2014-2018). Loading of total organic carbon (TOC) and dissolved inorganic carbon (DIC) to estuaries included riverine discharge and lateral exchange from tidal wetlands.

Decadal acidification in a subtropical coastal area under chronic eutrophication.

Coastal acidification is often much more intense than ocean acidification due to eutrophication. To better understand the relationship between long-term coastal acidification (CA) and coastal eutrophication (CE), in-situ monthly data over the past three decades (1986-2017) were analyzed from Hong Kong Coast (HKC). The coastwide annual mean pH change (ΔpH) was estimated at -0.

Photostable and biocompatible AIE-active conjugated polyelectrolytes for efficient heparin detection and specific lysosome labelling.

A series of new conjugated polyelectrolytes (CPEs) with emissive tetraphenylethene-containing backbones and specific targeting pendants are synthesized and characterized. These new CPEs exhibit prominent aggregation-induced emission (AIE) properties, high photostability and low cytotoxicity, and can efficiently detect heparin and stain lysosomes in living cells.

[Impact of Pseudomonas aeruginosa gene PA1550 on its swimming and twitching motility].

Objective: Studying the genes involved in swimming and twitching motility of Pseudomonas aeruginosa.

Methods: We used Mu transposition technique, gene cloning, nucleotide sequencing and the trans-complementation experiment to study the genes involved in twitching motility and swimming motility of P. aeruginosa strain PA68 isolated from a patient with bronchiectasis.

Identification and functional characterization of pfm, a novel gene involved in swimming motility of Pseudomonas aeruginosa.

Pseudomonas aeruginosa, an important opportunistic pathogen, has a single polar flagellum which is an important virulence and colonization factor by providing swimming motility. This paper describes the functional characterization of a novel gene pfm (PA2950) of P. aeruginosa.

Identification of a new gene PA5017 involved in flagella-mediated motility, chemotaxis and biofilm formation in Pseudomonas aeruginosa.

Flagella-mediated motility is recognized as one of the major factors contributing to virulence in Pseudomonas aeruginosa. During a screening of a mini-Mu transposon mutant library of P. aeruginosa PA68, a mutant partially deficient in swimming and swarming motility was identified in a new locus that encodes a predicted protein of unknown function annotated PA5017 in the P.

Influence of ptsP gene on pyocyanin production in Pseudomonas aeruginosa.

A pyocyanin overproducer with insertional inactivation of ptsP gene was isolated from a mini-Mu insertion library in Pseudomonas aeruginosa PA68. The mutation was complemented by a functional ptsP gene in trans. The pyocyanin-overproducing phenotype was also found in a ptsP mutant constructed by gene replacement in the P.

Identification of two new genes involved in twitching motility in Pseudomonas aeruginosa.

Mu transposition complexes were used for transposon mutagenesis of Pseudomonas aeruginosa strain PA68. Mu DNA transposition complexes were assembled with MuA transposase and an artificial mini-Mu transposon in vitro, and introduced into Pseudomonas aeruginosa by electroporation. Eight mutants deficient in twitching motility were isolated.