Publications by authors named "Hongmin Lei"
Article Synopsis
- A new DNA structure called ASDN (acid-stimulated self-assembly DNA nanonetwork) is designed for detecting the miRNA-221 biomarker in cancer cells and providing high-resolution imaging.
- ASDN self-assembles only in the acidic environment typical of cancer cells, allowing it to specifically target and enter these cells while avoiding noncancerous ones.
- The presence of miRNA-221 activates the ASDN, which enhances fluorescence signals, enabling sensitive detection of this biomarker at a very low concentration, thereby supporting cancer research and diagnostics.
Herein, a fluorescence light-up 3D DNA walker (FLDW) was powered and accelerated by endogenous adenosine-5'-triphosphate (ATP) molecules to construct a biosensor for sensitive and rapid label-free detection and imaging of microRNA-221 (miRNA-221) in malignant tumor cells. Impressively, ATP as the driving force and accelerator for FLDW could significantly accelerate the operation rate of FLDW, reduce the likelihood of errors in signaling, and improve the sensitivity of detection and imaging. When FLDW was initiated by output DNA H1-op transformed by target miRNA-221, G-rich sequences in the S strand, anchored to AuNP, were exposed to form G-quadruplexes (G4s), and thioflavin T (ThT) embedded in the G4s emitted intense fluorescence to realize sensitive and rapid detection of target miRNA-221.
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