Bland-Altman agreement analysis between CT predicted and surgical peritoneal cancer index in pseudomyxoma peritonei of appendiceal origin.

Peritoneal cancer index (PCI) is the surgical variable most commonly used to quantify the extent of peritoneal metastases for pseudomyxoma peritonei (PMP) patients. The present study aimed to investigate the agreement between CT predicted and surgical PCI by the Bland-Altman method for PMP of appendiceal origin. A total of 167 PMP patients of appendiceal origin were included between 2016 and 2021.

Bioconversion of vitamin D to bioactive calcifediol and calcitriol as high-value compounds.

Biological catalysis is an important approach for the production of high-value-added compounds, especially for products with complex structures. Limited by the complex steps of chemical synthesis and low yields, the bioconversion of vitamin D (VD) to calcifediol and calcitriol, which are natural steroid products with high added value and significantly higher biological activity compared to VD, is probably the most promising strategy for calcifediol and calcitriol production, and can be used as an alternative method for chemical synthesis. The conversion efficiency of VD to calcifediol and calcitriol has continued to rise in the past few decades with the help of several different VD hydroxylases, mostly cytochrome P450s (CYPs), and newly isolated strains.

Development of a new water-soluble fluorescence probe for hypochlorous acid detection in drinking water.

Responsive small-molecule fluorescence probe specific for target analyte detection is an emerging technology for food safety and quality analysis. In this work, we report a new water soluble small-molecule fluorescence probe () for the detection of hypochlorous acid (HOCl) in drinking water samples. Probe was developed by coupling of a glucosamine into 10-methyl-10H-phenothiazine fluorophore with a HOCl-responsive C=N bond.

A Novel Fluorescence Probe for the Reversible Detection of Bisulfite and Hydrogen Peroxide Pair in Vitro and in Vivo.

The detection of changes in the reactive oxygen species (ROS)/reactive sulfur species (RSS) couple is important for studying the cellular redox state. Herein, we developed a 1,8-naphthalimide-based fluorescence probe (NI) for the reversible detection of bisulfite (HSO ) and hydrogen peroxide (H O ) in vitro and in vivo. NI has been designed with a reactive ethylene unit which specifically reacts with HSO by a Michael addition reaction mechanism, resulting in the quenching of yellow fluorescence at 580 nm and the appearing of green fluorescence at 510 nm upon excitation at 500 nm and 430 nm, respectively.

Red-Emission Probe for Ratiometric Fluorescent Detection of Bisulfite and Its Application in Live Animals and Food Samples.

Key roles of bisulfite (HSO ) in food quality assurance and human health necessitate a reliable analytical method for rapid, sensitive, and selective detection of HSO . Herein, a new red-emitting ratiometric fluorescence probe, , is reported for sensitive and selective detection of HSO in food samples and live animals. Probe recognizes HSO via a 1,4-nucleophilic addition reaction.

A Copper (II) Ensemble-Based Fluorescence Chemosensor and Its Application in the 'Naked-Eye' Detection of Biothiols in Human Urine.

Quick and effective detection of biothiols in biological fluids has gained increasing attention due to its vital biological functions. In this paper, a novel reversible fluorescence chemosensor (-Cu) based on a benzocoumarin-Cu ensemble has been developed for the detection of biothiols (Cys, Hcy and GSH) in human urine. The chemosensing ensemble (-Cu) contains a 2:1 stoichiometry structure between fluorescent ligand and paramagnetic Cu.

Effect of grain morphology on the degradation behavior of Mg-4 wt% Zn alloy in Hank's solution.

The degradation behavior of Mg-4 wt% Zn alloy with three different microstructures was examined in Hank's solution at 37 °C by electrochemical measurements and immersion tests in this study. The results show that the sample with cellular structure exhibits a more positive corrosion potential, lower corrosion current density, larger impedance and more protective film than samples with columnar dendritic and equiaxed dendritic structure. The higher corrosion resistance is attributed to the preferred orientation, eliminating susceptible grain boundaries and reduced secondary phases.

Two D-π-A Schiff-Base-Functionalized Silica Gel Adsorbents for Preconcentration of Copper Ions in Foods and Water for Detection.

Copper is an essential element in many biological processes and plays an important role in carbohydrate and lipid metabolism. Excess or deficiency of Cu ions can cause disturbances in cellular homeostasis and damage the central nervous system. Here, for the first time, two functionalized silica gel (SG-A and SG-B) adsorbents were prepared and tested for copper detection via the reactions of chlorinated silica gel with two novel D-π-A Schiff base compounds: 2-amino-3-(quinolin-2-ylmethyleneamino)maleonitrile (A) and 2-(4-(diethylamino)-2-hydroxybenzylideneamino)-3-aminomaleonitrile (B) in the thionyl chloride solution, respectively.

Effects of -lysine·HSO product on the intestinal morphology and liver pathology using broiler model.

Background: Lysine is used widely in livestock production due to the shortage of feed protein resources. -lysine·HSO contains -lysine sulphate as well as fermentation co-products which contain other amino acids and phosphorus. However, there are few articles about -lysine·HSO product regarding intestinal morphology and liver pathology of broiler chickens.

Quantification of Gly m 5.0101 in Soybean and Soy Products by Liquid Chromatography-Tandem Mass Spectrometry.

Gly m 5.0101, the alpha subunit of β-conglycinin, is one of the major allergens found in soybeans that has been identified as causing an allergic reaction. Here, we developed a quantification method of Gly m 5.

Rapid Response Fluorescence Probe Enabled In Vivo Diagnosis and Assessing Treatment Response of Hypochlorous Acid-Mediated Rheumatoid Arthritis.

Diagnosis and early assessment of the treatment response of rheumatoid arthritis (RA) necessitates a reliable bioanalytical method for rapid, sensitive, and specific detection of the hypochlorous acid (HOCl) biomarker in inflammatory diseases. Herein, two fluorescence probes, Probe- and Probe- are developed for quantitative monitoring and visualization of inflammatory response-related HOCl levels in vitro and in vivo. In the presence of HOCl, fluorescence "OFF-ON" response is obtained for both the probes as a result of specific HOCl-triggered C=N bond cleavage reaction.

Responsive Fluorescence Probe for Selective and Sensitive Detection of Hypochlorous Acid in Live Cells and Animals.

The development of effective bioanalytical methods for rapid, sensitive and specific detection of HOCl in vitro and in vivo plays a key role for better understanding the roles of this molecule in normal and diseased conditions, but remains challenging due to the highly reactive nature of HOCl and the complicated biological conditions. In this work, a new fluorescence probe, PQI, was developed for monitoring of the HOCl level in biological samples. PQI was easily synthesized by a one-step condensation reaction.

A highly sensitive electrochemical biosensor for phenol derivatives using a graphene oxide-modified tyrosinase electrode.

The fabrication, characterization and analytical performance were investigated for a phenol biosensor based on the covalent bonding of tyrosinase (TYR) onto a graphene oxide (GO)-modified glassy carbon electrode (GCE) via glutaraldehyde (GA). The surface morphology of the modified electrode was studied by atomic force microscope (AFM) and field-emission scanning electron microscopy (FE-SEM). The fabricated TYR/GA/GO/GCE biosensor showed very good stability, reproducibility, sensitivity and practical usage.

Valine Supplementation in a Reduced Protein Diet Regulates Growth Performance Partially through Modulation of Plasma Amino Acids Profile, Metabolic Responses, Endocrine, and Neural Factors in Piglets.

The objective of this study was to investigate whether valine (Val) supplementation in a reduced protein (RP) diet regulates growth performance associated with the changes in plasma amino acids (AAs) profile, metabolism, endocrine, and neural system in piglets. Piglets or piglets with a catheter in the precaval vein were randomly assigned to two treatments, including two RP diets with standardized ileal digestible (SID) Val:Lysine (Lys) ratio of 0.45 and 0.

A fast response fluorescence probe specific for hypochlorous acid detection and its applications in bioimaging.

Quantitative detection and visualization of hypochlorous acid (HOCl) in biological samples has emerged as a hot topic in biochemical research because of the important role of this biomolecule in live organisms. In this contribution, a novel 1,8-naphthalimide-based fluorescence probe, DNPH-NA, was designed and synthesized for HOCl detection in aqueous solutions and in biological systems. In the presence of HOCl, significant changes in absorption and fluorescence spectra were observed, which allow for the quantitative detection of HOCl in PBS buffer.

Numerical simulation of non-dendritic structure formation in Mg-Al alloy solidified with ultrasonic field.

The formation of non-dendritic structure of Mg alloy solidified with ultrasonic treatment was investigated by numerical simulation and experiment. The models of nucleation and crystal growth involved the effects of ultrasonic cavitation and acoustic streaming were built. Based on the models, the grain refinement and the microstructure change from dendrite to non-dendritic structure of a Mg-Al alloy were numerically simulated by cellular automata method.

Lactobacillus reuteri I5007 Modulates Intestinal Host Defense Peptide Expression in the Model of IPEC-J2 Cells and Neonatal Piglets.

Modulation of the synthesis of endogenous host defense peptides (HDPs) by probiotics represents a novel antimicrobial approach for disease control and prevention, particularly against antibiotic-resistant infections in human and animals. However, the extent of HDP modulation by probiotics is species dependent and strain specific. In the present study, The porcine small intestinal epithelial cell line (IPEC-J2) cells and neonatal piglets were used as in-vitro and in-vivo models to test whether I5007 could modulate intestinal HDP expression.

A ratiometric fluorescence probe for imaging sulfur dioxide derivatives in the mitochondria of living cells.

Although sulfur dioxide (SO) plays an essential role in several physiological processes, monitoring of intracellular SO at subcellular levels remains challenging due to the lack of rapid and sensitive methods for its quantification in a 100% aqueous solution. Herein, a new hemicyanine dyes-based fluorescence probe, NBD-Id, was designed and synthesized for the detection of SO derivatives in pure aqueous solution and living cells. By virtue of a specific 1,4-addition reaction of SOHSO and the polymethine chain of hemicyanine, significant changes in the absorption and fluorescence emission spectra were observed in less than 20 seconds.

Synthesis and Application of an Aldazine-Based Fluorescence Chemosensor for the Sequential Detection of Cu²⁺ and Biological Thiols in Aqueous Solution and Living Cells.

A fluorescence chemosensor, 2-hydroxy-1-naphthaldehyde azine (HNA) was designed and synthesized for sequential detection of Cu(2+) and biothiols. It was found that HNA can specifically bind to Cu(2+) with 1:1 stoichiometry, accompanied with a dramatic fluorescence quenching and a remarkable bathochromic-shift of the absorbance peak in HEPES buffer. The generated HNA-Cu(2+) ensemble displayed a "turn-on" fluorescent response specific for biothiols (Hcy, Cys and GSH) based on the displacement approach, giving a remarkable recovery of fluorescence and UV-Vis spectra.

Reversible and Selective Fluorescence Detection of Histidine Using a Naphthalimide-Based Chemosensing Ensemble.

We described a new ensemble-approach-based chemosensor, NCH-Cu(2+), for highly selective and reversible detection of histidine (His) in aqueous solution and live cells. The ligand NCH exhibited specific binding with Cu(2+) ions over other metal ions, accompanied with a 92.2% fluorescence quenching.

A highly selective and sensitive ON-OFF-ON fluorescence chemosensor for cysteine detection in endoplasmic reticulum.

A new complex between coumarin-based ligand (CL) and copper ion has been prepared and applied to be an ON-OFF-ON reversible fluorescence chemosensor for the detection of Cys with high sensitivity and specificity. In HEPES buffer, CL displayed high affinity towards Cu(2+) ion over other physiological and environmental metal ions, accompanied with a 98.4% of fluorescence quenching.

A reversible fluorescence chemosensor for sequentially quantitative monitoring copper and sulfide in living cells.

We report a novel, selective and sensitive strategy for the sequentially "ON-OFF-ON" fluorescent detection of Cu(2+) and S(2-) based on a fluorescein derivative, FL. The specific binding of FL towards Cu(2+) in aqueous and biological media led to the intensive green fluorescence quenching and a notable increase of the absorbance maximum at 480 nm. In the presence of S(2-), the intensity and overall pattern of the fluorescence emission and UV-vis spectra of FL-Cu(2+) ensemble were recovered since the abolishment of paramagnetic Cu(2+).

Fluorescence detection of Fe(3+) ions in aqueous solution and living cells based on a high selectivity and sensitivity chemosensor.

Although ferric ion (Fe(3+)) performs critical roles in diverse biochemical processes in living systems, its physiological and pathophysiological functions have not been fully explored due to the lack of methods for quantification of Fe(3+) ions in biological system. In this work, a highly sensitive and selective fluorescence chemosensor, L, was developed for the detection of Fe(3+) ions in aqueous solution and in living cells. L was facile synthesized by one step reaction and well characterized by NMR, API-ES, FT-IR, and elementary analysis.

NBD-based fluorescent chemosensor for the selective quantification of copper and sulfide in an aqueous solution and living cells.

Chemosensors play important roles in cation and anion recognition in biological, industrial, and environmental processes. Although many efforts have been made to develop artificial fluorescent receptors for Cu(2+) and S(2-), their applications in the detection in bulk solutions are limited. In this work, we report a novel fluorescence chemosensor (NL) based on the 7-nitrobenz-2-oxa-1,3-diazole (NBD) fluorophore for the quantification of Cu(2+) and S(2-) in single intact cells.

Correlation of abdominal fat distribution with different types of diabetes in a Chinese population.

To investigate abdominal fat distribution in Chinese subjects with diabetes and its correlation with different types of diabetes. A total of 176 diabetic subjects were enrolled, 92 with type 1 and 84 with type 2, with a mean age of 27.41 and 49.