Biological characteristics of a precocious line of Eimeria tenella.

The Eimeria tenella Yulin strain (EtYL), which is sensitive to most anti-coccidial drugs, was isolated in the Yulin area of Guangxi, China. Then, Eimeria tenella Yulin precocious line (pEtYL), a precocious line with a prepatent period of 108 h, was obtained through early selection. The biological characteristics of pEtYL, including its morphology, purity, oocyst excretion curve, reproductive capacity, pathogenicity, immunogenicity, and preservation time, were comprehensively analyzed.

The Effect of Contrast-enhanced Ultrasound Vessels and Surgical Drains Guidance Percutaneous Catheter Drainage in the Treatment of Pyogenic Liver Abscess.

Background: Pyogenic liver abscess (PLA) is a purulent disease caused by microbial contamination of liver parenchyma and includes amoebic liver abscess, fungal liver abscess, and the most common bacterial liver abscess.

Objective: Explore the efficacy of contrast-enhanced ultrasound (CEUS) via vessels and surgical drain guidance percutaneous catheter drainage (PCD) in the treatment of pyogenic liver abscesses (PLA).

Materials And Methods: A total of 86 PLA patients who underwent PCD treatment in our hospital from May 2018 to February 2023 were retrospectively selected.

IgG4-related disease with massive pericardial effusion diagnosed clinically using FDG-PETCT: a case report.

Background: IgG4-related disease (IgG4-RD) is a systemic inflammatory disease which involves various organs such as the pancreas, lacrimal gland, salivary gland, retroperitoneum, and so on. These organs can be affected concomitantly. 18-Fluorodeoxyglucose positron emission tomography computed tomography (FDG-PETCT) is a systemic examination which can identify active inflammation and detect multiple organ involvement simultaneously.

Atmospheric-Pressurized Process for Dimethyl Carbonate/Methanol Separation with and without Heat Integration: Design and Control.

Process economy and dynamic controllability are critical for DMC/MeOH separation via the PSD process. In this paper, rigorous steady-state and dynamic simulations of atmospheric-pressurized process for DMC/MeOH separation with no, partial, and full heat integration have been carried out with Aspen Plus and Aspen Dynamics. Further investigations have been conducted into the economic design and dynamic controllability of the three neat systems.

Nanotopography-Modulated Epithelial Cell Collective Migration.

Matrix nanotopography plays an essential role in regulating cell behaviors including cell proliferation, differentiation, and migration. While studies on isolated single cell migration along the nanostructural orientation have been reported for various cell types, there remains a lack of understanding of how nanotopography regulates the behavior of collectively migrating cells during processes such as epithelial wound healing. We demonstrated that collective migration of epithelial cells was promoted on nanogratings perpendicular to, but not on those parallel to, the wound-healing axis.

Sporulation rate and viability of Eimeria tenella oocysts stored in potassium sorbate solution.

In order to find a new preservation solution for avian coccidial oocysts that can replace potassium dichromate (KCrO) solution, Eimeria tenella oocysts were preserved in 0.1 to 10% potassium sorbate (CHKO) solution in this study. The results showed that there was no significant difference between the sporulation rate of E.

Selection and characterization of a Vibrio parahaemolyticus OmpU antibody by phage display.

Vibrio parahaemolyticus (V. parahaemolyticus) is a well-known food-borne human pathogen that can cause a variety of clinical manifestations after the consumption of raw or undercooked seafoods. The crucial roles of Vibrio OmpU in bacterial pathogenesis have been found in recent studies.

Achieving Efficient CO Electrochemical Reduction on Tunable In(OH)-Coupled CuO-Derived Hybrid Catalysts.

Tunable In(OH)-coupled CuO-derived hybrid catalysts are facilely synthesized to boost the selectivity and efficiency of the electrochemical CO reduction reaction (CORR). The maximum faradaic efficiency (FE) of 90.37% for CO production is achieved at -0.

Staff Training Reduces the Use of Physical Restraint in Mental Health Service, Evidence-based Reflection for China.

Object: The purpose of this article was to synthesize the evidence regarding the reduction of physical restraint, and to seek some practical recommendations based on the current situation in China.

Method: Nine databases were retrieved; these were PubMed, CINAHL, MEDLINE, Trip Database, PsysINFO, Cochrane Library, CNKI (Chinese database), Wanfang (Chinese database) and CBM (Chinese database) respectively. The selected articles were screened manually, and the identified researches were appraised through Review manager 5.

Physical restraints: An ethical dilemma in mental health services in China.

Given that physical restraints cause adverse effects to patients and nurses, their wide and frequent use has resulted in various clinical and ethical controversies in mental health services. The rate of physical restraints is significantly higher in China than in other countries. Some western and domestic media blamed relevant institutions for compromising the basic rights of psychiatric patients.

Endostar inhibits ascites formation and prolongs survival in mouse models of malignant ascites.

Endostar, a modified recombinant human endostatin, inhibits the growth of a variety of tumors by suppressing neovascularization. Vascular endothelial growth factor (VEGF) has an important role in malignant ascites formation. In order to determine whether Endostar can suppress the formation of ascites and prolong survival times, mouse models of malignant ascites were established using S180 and H22 tumor cells.

[Therapeutic features of endostar, a modified endostatin, on ascites tumor in mice].

Objective: To investigate the effect of endostar in controlling ascites tumor formation in mice.

Methods: Mouse models bearing ascites tumors were established via intraperitoneal injection of H22 and S180 cell lines. Eighty-eight ICR mice were randomly assigned into 4 groups, namely the control group (0.

Macroporous ordered titanium dioxide (TiO2) inverse opal as a new label-free immunosensor.

Photonic crystal sensing materials have been validated that they are very sensitive to refractive index changes. Herein, three-dimensionally ordered macroporous (3DOM) (>50 nm) TiO2 inverse opal film has been fabricated by the self-assembly technique. Based on the TiO2 inverse opal film, the optical spectrometer was established for label-free immunosensor.

Electrospun polymer nanofibers as a solid-phase extraction sorbent for the determination of trace pollutants in environmental water.

This paper describes the novel preparation of three kinds of nanofibers [poly(styrene-co-methacrylic acid), poly(styrene-co-p-styrene sulfonate), polystyrene] investigated as solid-phase extraction (SPE) sorbents to extract six compounds (nitrobenzene, 2-naphthol, benzene, n-butyl p-hydroxybenzoate, naphthalene, p-dichlorobenzene) in environmental water by high-performance liquid chromatography. Parameters affecting extraction efficiency were investigated in detail to explore the extraction mechanism of the nanofibers. Under optimized conditions, six compounds followed an excellent linear relationship in the range 10-5,000 ng mL(-1) with coefficients of determination (r (2)) greater than 0.

[Therapeutic effects of cryoablation, radiofrequency ablation, and microwave coagulation against VX2 liver cancer: a comparative study in rabbits].

Objective: To Compare the therapeutic effects of cryocareTM cryoablation, radiofrequency ablation(RFA), and and microwave coagulation (MCT) in rabbits with VX(2) liver cancer.

Methods: Forty-five rabbits with VX(2) liver cancer were randomly and equally allocated into 5 groups to receive treatment with cryocare cryoablation (group A), radiofrequency ablation (group B), microwave coagulation (group C), surgical resection (group D) and control group (group E), respectively. The residual tumor tissues and metastasis (intrahepatic, lung, abdominal lymphoid node, and abdominal implantation) were observed after the treatments, with also detection of soluble interleukin-2 receptor ( sIL-2R) and recording of the survival time of the rabbits.

[Adriamycin combined with hyperthermia inhibits transplanted B cell lymphoma in nude mice].

Objective: To study the effect of adriamycin combined with hyperthermia on tumor formation and growth of human B lymphoma cell line (Raji) in nude mice.

Methods: Twenty-four BALB/C nude mice were divided into control group (37 degrees celsius;), chemotherapy group (37 degrees celsius;+ADM), hyperthermia group (42 degrees celsius;) and thermochemotherapy group (42 degrees celsius;+ADM), and in each mouse, 5 x 10(6) Raji cells were injected subcutaneously. The time and rate of tumor formation were observed, the tumor diameter was measured every 3 days and the tumor volume calculated to obtain the growth curves of the tumors.

[Effect of hyperthermia in combination with chemotherapy on K562/AO2 cells in vitro].

This study was purposed to investigate the inhibitory effect, apoptosis, Bcl-2 and P-gp expression of K562/AO2 cells by hyperthermia combined with adriamycin. The working concentration of adriamycin against K562/AO2 was determined by MTT assay. The hyperthermia and chemotherapy were used alone or in combination, then the cell survival rate was detected at 48 hours.

[Expression of NKG2D ligands in multidrug-resistant nasopharyngeal carcinoma cell line CNE2/DDP and their effects on cytotoxicity of natural killer cells].

Objective: To analyze the expression of NKG2D ligands on human nasopharyngeal carcinoma cell line CNE2 and the multidrug-resistant lin CNE2/DDP and investigate its impact on cytotoxicity of natural killer (NK) cells.

Methods: Expression of NKG2D ligands on the surface of CNE2 and CNE2/DDP cells was analyzed by flow cytometry, and their HLA genotypes, along with inhibitory killer cell immunoglobulin-like receptors (KIRs) expressed on NK cells from 5 healthy donors, were determined by PCR with sequence specific primers. Cytotoxicity of NK cells against CNE2 and CNE2/DDP cells was evaluated by LDH-releasing assay at different effector-to-target ratios (E:T).

[Thermotherapy enhances the sensitivity of lymphoma cell line Raji to chemotherapy in vitro].

Objective: To observe the effect of thermotherapy on the intracellular adriamycin concentration and apoptosis of Raji cells in vitro.

Methods: The working concentration of adriamycin against Raji cells was determined with MTT assay. Raji cells were subjected to thermotherapy (at 40 degrees Celsius;, 41 degrees Celsius; or 42 degrees Celsius;) and chemotherapy with adriamycin alone or in conjunction, and the cell survival rates were determined 48 h after the treatment.

[Expression of HLA class I molecules and MHC class I chain-related molecules A/B in K562 and K562/AO2 cell lines and their effects on cytotoxicity of NK cells].

The study was aimed to investigate the expression of HLA class I molecules and MHC class I chain-related molecules A/B (MICA/MICB) in K562 and adriamycin (ADM)-resistant K562 cell lines (K562/AO2) and their effect on cytotoxicity of NK cells. Expression of HLA class I molecules and MICA/MICB on the surface of K562 and K562/AO2 cell lines were analyzed by flow cytometry. Cytotoxicity of NK cells (isolated from 3 healthy persons) against K562 and K562/AO2 cells were detected by LDH releasing assay at different effect-to-target cell ratios (E:T).