The Role of PI3K/AKT/HIF-1α Pathway in the Effect of Nano-TiO on Lactate Production in TM4 Cells.

Titanium dioxide nanoparticles (nano-TiO) can cause a reduction in sperm counts, and lactate production in Sertoli cells plays a key role in spermatogenesis. The aim of this study was to evaluate the effect of nano-TiO on lactate production in mouse Sertoli cell line (TM4 cells) and to investigate whether the effect is mediated through the PI3K/AKT/HIF-1α pathway. After TM4 cells were treated with different concentrations of nano-TiO for 48 h, cell viability, contents of glucose and lactate, and the expression levels of GLUT3 and key enzymes (HK1, HK2, PFKM, ENO1, LDH) during lactate production were detected.

Study on the mechanisms of defective spermatogenesis induced by TiO NPs based on 3D blood-testis barrier microfluidic chip.

Titanium dioxide nanoparticles (TiO NPs) can reduce sperm number, but the mechanisms of defective spermatogenesis induced by TiO NPs have not been studied through cell-cell interactions at present. A kind of biomimetic three-dimensional blood-testis barrier microfluidic chip capable of intercellular communication was constructed with soft lithography techniques, including Sertoli cell (TM4), spermatogonia (GC-1) and vascular endothelial cell units, to study the mechanisms of TiO NPs-induced defective spermatogenesis. TM4 and GC-1 cells cultured in TiO NPs exposure and control chips were collected for transcriptomics and metabonomics analysis, and key proteins and metabolites in changed biological processes were validated.

Titanium Dioxide Nanoparticles Induce Cell Cycle Arrest and Apoptosis through Inhibiting PI3K/AKT/mTOR Pathway in Spermatogonia.

Titanium dioxide nanoparticles (TiO NPs) can result in the reduction of sperm numbers, but the mechanisms have not been well elucidated. The purpose of this study was to investigate the effects of TiO NPs on cell cycle and apoptosis in spermatogonia and to explore the role of PI3K/AKT/mTOR signaling pathway in this process. The mouse spermatogonia cell line (GC-1) was treated with TiO NPs at different concentrations (0, 25, 50, 75 and 100 μg/mL) for 24 h to detect cell viability, cell cycle, apoptosis, and key proteins related to cell cycle and PI3K/AKT/mTOR signaling pathway.

Effects of nano-titanium dioxide on calcium homeostasis and : a systematic review and meta-analysis.

With the extensive application of titanium dioxide nanoparticles (TiO NPs), their impacts on calcium homeostasis have aroused extensive attention from scholars. However, there are still some controversies in relevant reports. Therefore, a systematic review was performed followed by a meta-analysis to explore whether TiO NPs could induce the imbalance in calcium homeostasis and through Revman5.

Effect of Titanium Dioxide Nanoparticles on Mammalian Cell Cycle : A Systematic Review and Meta-Analysis.

Although most studies that explore the cytotoxicity of titanium dioxide nanoparticles (nano-TiO) have focused on cell viability and oxidative stress, the cell cycle, a basic process of cell life, can also be affected. However, the results on the effects of nano-TiO on mammalian cell cycle are still inconsistent. A systematic review and meta-analysis were therefore performed in this research based on the effects of nano-TiO on the mammalian cell cycle to explore whether nano-TiO can induce cell cycle arrest.

The Key Technologies of Road Elevation Detection Based on Sensor Fusion.

The detection of long-distance pavement elevation undulation is the main data basis for pavement slope detection and flatness detection, and is also the data source for 3D modeling and quality evaluation of pavement surfaces. The traditional detection method is to use a level and manual coordination to measure; however, the detection accuracy is low and the detection speed is slow. In this paper, the high-speed non-contact vehicle-mounted road undulationelevation detection method is adopted, combined with the advantages of each sensor measurement; three methods are proposed to detect the road undulation elevation: rotary encoders, accelerometers, attitude sensor data fusion detection; GPS RTK detection; and Kalman filtering detection.

Nanosized Titanium Dioxide Induced Apoptosis and Abnormal Expression of Blood-Testis Barrier Junction Proteins Through JNK Signaling Pathway in TM4 Cells.

Nanosized titanium dioxide (nano-TiO) has been widely used in consumer products. It can cross the blood-testis barrier (BTB), and it has adverse effects on the male reproductive system. However, the specific mechanism has not been fully elucidated.

Genotoxicity evaluation of silica nanoparticles in murine: a systematic review and meta-analysis.

Silica nanoparticles (SiNPs) have been widely used in nanotechnology, and more attention has been paid to their safety evaluation. However, there are still inconsistent conclusions about the genotoxicity of SiNPs. A systematic review was conducted to explore whether SiNPs have genotoxicity followed by a meta-analysis of and murine genotoxicity tests.

Design and synthesis of novel spirooxindole-indenoquinoxaline derivatives as novel tryptophanyl-tRNA synthetase inhibitors.

In the current study, we used an integrated approach combining bioinformatics, rational drug design, one-pot synthesis, and biological experiments in vitro for the potential discovery of novel tryptophanyl-tRNA synthetase (TrpRS) inhibitors. Atom economic and diastereoselective syntheses were used to generate several Spirooxindole-indenoquinoxaline derivatives in situ from isatin and amino acids viz. proline, phenylglycine, and sarcosine through targeting the 1,3-dipolar cycloaddition of azomethine ylides.