Publications by authors named "Hongliang Liu"

Article Synopsis
  • A study analyzed 26,544 single nucleotide polymorphisms (SNPs) in 291 FRGs and identified 661 SNPs linked to overall survival (OS) in NSCLC patients, later validating these findings in a separate patient group.
  • Two SNPs, FER rs7716388 A>G and SULF1 rs11785839 G>C, were found to be significantly associated with improved survival rates, suggesting that these genetic variants could serve as progn
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African swine fever (ASF), which is caused by the African swine fever virus (ASFV). There are currently no effective vaccines or therapies for ASF; rapid diagnosis has become an important way to control the disease. The p11.

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Tetracycline (Tc) antibiotics, a class of synthetically produced broad-spectrum antimicrobial drugs, have been widely used in animal husbandry, leading to their widespread presence in animal-derived foods. However, misuse, overuse, and non-compliance with withdrawal periods in animal farming have resulted in excessive Tc residues in these foods, which can cause various adverse reactions in humans, induce bacterial resistance, and pose a significant threat to public health. Consequently, the detection of Tc antibiotic residues in animal-derived food has become a critical issue.

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Objective: Patients with clear cell renal carcinoma (ccRCC) typically have poor prognosis. Glycosylation modification plays an important role in ccRCC. This study aimed to develop a novel signature for predicting ccRCC prognosis based on glycosylation-related genes (GRGs).

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The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) led to the global spread of coronavirus disease 2019 (COVID-19), creating an urgent need for updated methods to evaluate immune responses to vaccines and therapeutic strategies. In this study, we introduce a novel cell-free, virus-free SARS-CoV-2 neutralizing antibody ELISA (NAb-ELISA), which is based on competitive inhibition of the receptor binding domain (RBD) of spike protein binding to the angiotensin-converting enzyme 2 (ACE2) receptor. In this method, site-specific biotinylated hACE2-Fc-Avi recombinant protein is immobilized onto a 96-well plate for capture, and the RBD-Fc-vHRP recombinant proteins serve as detection probes.

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Unlabelled: African swine fever virus (ASFV) is a highly contagious and often fatal pathogen that poses a significant threat to the swine industry worldwide. The H171R protein, a structural component of ASFV, plays crucial roles in viral assembly, host cell entry, and modulation of the host immune response. This study aimed to comprehensively characterize the linear B-cell epitopes on the H171R protein to facilitate the development of diagnostic tools and subunit vaccines against ASFV.

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African Swine Fever Virus (ASFV), a highly contagious DNA virus, causes severe economic losses to the global swine industry. The ASFV p15 protein, which is found in the core shell, is essential to the assembly of viral particles. In addition, protein p15 is a candidate target for the development of diagnostic reagents for African Swine Fever (ASF) because of its excellent immunogenicity.

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  • - Genomic imprinting, crucial for mammalian development, relies on PGC7, a maternal factor that maintains DNA methylation by binding to dimethylated histone H3K9 (H3K9me2) in zygotes and stem cells.
  • - The study identifies that PGC7 interacts with HP1BP3, a novel histone H1 family member, specifically through its C-terminal tail, facilitating recruitment to the Meg3 differentially methylated region (DMR) within the Dlk1-Dio3 imprinted domain.
  • - The combined action of PGC7 and HP1BP3 prevents the binding of DNA methyltransferase DNMT3A, therefore preserving the methylation status of the
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  • T cell exhaustion is linked to decreased effectiveness of immune checkpoint inhibitors in lung cancer, which has the highest mortality rate among cancers, although the genetic variants related to T cell exhaustion in non-small cell lung cancer (NSCLC) prognosis are not well studied.!* -
  • A two-stage analysis using existing genome-wide association study (GWAS) datasets identified 1,721 single nucleotide polymorphisms (SNPs) related to T cell exhaustion that were associated with overall survival in NSCLC patients, with 125 confirmed significant after further validation.!* -
  • Three specific SNPs (rs10493829, rs2239193, and rs3136651) showed a significant association with overall survival, highlighting that certain genotypes
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Prehospital rescue of accidental massive bleeding is crucial for saving lives. However, currently available hemostatic materials are still in infancy in treating accidental bleeding due to the challenges in fully satisfying the complex outdoor hemostatic requirements. Herein, we designed an epidermal growth factor (EGF)- incorporated, microparticle-formed, high-strength, dynamic environment-stable hemostatic gel system for prehospital rescue.

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  • - Stretchable transparent electrodes (STEs) made from silver nanowires and PDMS are key materials for flexible electronics, but current manufacturing methods are inefficient for large-scale production.
  • - This research introduces a new approach using a gradient PDMS substrate with adjustable properties, improving the performance of STEs in terms of optoelectrical characteristics, stretchability, and stability.
  • - The developed STEs are versatile, with potential applications in electro-heating and electroluminescent devices, and offer a simpler, more effective fabrication method compared to traditional techniques.
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  • African swine fever (ASF) is a viral disease affecting pigs and wild boars, caused by the African swine fever virus (ASFV), and there is currently no effective vaccine available.
  • The study focuses on the structural protein p49, essential for ASFV’s capsid structure, and identifies it as a potential vaccine target by generating monoclonal antibodies (mAbs) using the hybridoma technique.
  • It was revealed that specific segments of the p49 protein (B4 and B5) contain major B cell epitopes which are conserved across ASFV strains, potentially aiding in the development of ASF vaccines.
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Ferroptosis, a form of regulated cell death, is characterized by iron-dependent lipid peroxidation. It is recognized increasingly for its pivotal role in both cancer development and the response to cancer treatments. We assessed associations between 370,027 single-nucleotide polymorphisms (SNPs) within 467 ferroptosis-related genes and survival of non-small cell lung cancer (NSCLC) patients.

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As an important parameter of the cellular microenvironment, the changes in mitochondrial viscosity are closely related to various life activities. Therefore, the development of fluorescent probes for test the changes of mitochondrial viscosity has great significance. In this study, we developed two fluorescent probes for the detection of the mitochondrial viscosity changes.

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Iodine and fluorine, as halogen elements, are often coexisting in water environments, with nearly 200 million people suffering from fluorosis globally, and, in 11 countries and territories, adolescents have iodine intakes higher than that required for the prevention of iodine deficiency disorders. It has been suggested that excess iodine and/or fluorine can affect thyroid health and intellectual development, especially in children, but their combined effect has been less studied in this population. This study investigated 399 school-age children in Tianjin, China, collected drinking water samples from areas where the school-age children lived, and grouped the respondents according to iodine and fluorine levels.

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Pancreatic cancer is a highly aggressive and lethal carcinoma. Circular RNAs (circRNAs) serve key regulatory functions in pancreatic cancer. Ferroptosis was induced by erastin treatment and analyzed by examining malondialdehyde (MDA), iron, Fe and glutathione (GSH).

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  • Marine mussels' strong adhesive properties have led to the innovative development of mussel-inspired injectable adhesive hydrogels, which are gaining traction in biomedical applications due to their minimally invasive nature.
  • These hydrogels, enriched with catechol groups, boast essential features like tissue adhesion, self-healing abilities, and antimicrobial effects, which enhance their usability in medical settings.
  • The review covers the adhesion mechanism of mussels, design strategies for these hydrogels, and their potential challenges, aiming to inspire new bioinspired designs for future biomedical uses.
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  • The study investigates how intraoperative temperatures, both hypothermia (below 36°C) and hyperthermia (above 37.3°C), affect the risk of postoperative pulmonary infections (PPI) and surgical site infections (SSI) in patients undergoing major non-cardiac surgeries under general anesthesia.
  • Findings revealed that longer durations of hypothermia (>90 min) and hyperthermia (>75 min) significantly increased the likelihood of PPI, while prolonged hypothermia (>195 min) and hyperthermia also raised the risk of SSI.
  • Overall, the research concluded that variations in body temperature during surgery are linked to higher rates of postoperative infections, emphasizing the need for careful temperature
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  • B cells are important in cancer progression, particularly non-small cell lung cancer (NSCLC), but the impact of genetic variants in immunity-related genes on patient survival is unclear.
  • The study analyzed over 10,000 genetic variants in B cell-related genes among nearly 1,200 NSCLC patients and identified 369 single nucleotide polymorphisms (SNPs) linked to overall survival, further validating 18 SNPs in an additional patient group.
  • Two specific SNPs were found to significantly influence patient survival, with certain unfavorable combinations of these genotypes leading to poorer outcomes, and gene expression analyses indicated that specific alleles correlate with higher gene activity in lung tissues.
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A label-free electrochemical immunosensor was developed to rapidly detect tilmicosin (TMC) residues in pork and milk. The immunosensor was constructed by immobilizing a high-affinity monoclonal antibody against TMC on an rGO-PEI-Ag nanocomposite-modified electrode. The rGO-PEI-Ag nanocomposites were prepared by mixing polyethyleneimine (PEI) modified reduced graphene oxide (rGO) with AgNO solution.

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The African swine fever virus (ASFV), a highly contagious pathogen responsible for African swine fever (ASF), causes significant economic losses in the global pork industry. Due to its large and complex structure, ASFV remains refractory to commercial vaccine development, necessitating the creation of rapid, sensitive, and specific diagnostic tools for disease control. In this study, quantum dots were conjugated to ASFV p72 protein to establish a fluorescent immunochromatographic assay for detecting ASFV-specific antibodies.

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ASFV is the only known double-stranded insect-borne DNA virus, which can rapidly infect domestic pigs and wild boars with ticks as transmission medium. Since it was first discovered in 1921, it quickly spread to all parts of the world and brought huge economic losses to the pig industry all over the world. At present, there is still no safe and effective vaccine for ASFV.

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  • Hypoxia plays a significant role in cancer aggression, impacting processes like blood vessel formation and immune response, yet the influence of genetic variants in hypoxia-related genes on survival in non-small cell lung cancer (NSCLC) remains unclear.
  • Researchers studied 16,092 single-nucleotide polymorphisms (SNPs) in 182 hypoxia-related genes among NSCLC patients, using data from two cancer studies to identify any survival associations.
  • They found that the ERRFI1 gene variant rs28624 was linked to lower overall survival rates in patients, indicating that certain genetic variations could affect how long individuals with NSCLC live, possibly by influencing gene expression.
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Synopsis of recent research by authors named "Hongliang Liu"

  • - Hongliang Liu's recent research primarily focuses on the identification and characterization of B-cell epitopes associated with African swine fever virus (ASFV), aiming to develop diagnostic tools and subunit vaccines to combat this significant threat to the swine industry! - Liu has also contributed to the field of cancer research, exploring genetic variants in T cell exhaustion-related genes and hypoxia-related genes to better understand their associations with non-small cell lung cancer survival outcomes, potentially guiding future therapeutic strategies! - Additionally, Liu's work extends into biomedical applications, including the development of innovative hemostatic materials and adhesive hydrogels for wound care, demonstrating a multidisciplinary approach that combines bioengineering with healthcare improvement!