Publications by authors named "Hongkai Qiu"
Highly pathogenic PRRSV (HP-PRRSV) emerged in China in 2006 and caused severe reproductive losses, particularly in late-term sows. To determine whether these reproductive failures were related to the susceptibility of late-term sows to HP-PRRV, 60- and 90-days of gestation sows were infected with HP-PRRSV isolate TA-12 (GenBank accession HQ417620). A monoclonal antibody specific to the C-terminal of the nucleocapsid protein was used to evaluate viral distribution by IHC.
View Article and Find Full Text PDFAvian hepatitis E virus (HEV) is related genetically and antigenically to human and swine HEVs and capsid protein of avian HEV shares approximately 48-49% amino acid sequence identities with those of human and swine HEVs. Six monoclonal antibodies (MAbs) were produced and used to locate different epitopes in the ORF2 region of aa 339-570 of avian HEV Chinese isolate. The results showed that five epitopes were located in the aa 339-414 region and one in the aa 510-515 region.
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- Two stable hybridoma cell lines were created that produce IgG1 monoclonal antibodies against goat IL-18.
- The antibodies, named 2E8 and 4C4, showed specific binding to goat IL-18 in assays like ELISA and Western blot, indicating they target different parts of the protein.
- These antibodies were effective in detecting goat IL-18 in various cell types and could also recognize IL-18 from other species, suggesting their potential use in studying inflammation and immune responses.