Regulation of protein thermal stability and its potential application in the development of thermo-attenuated vaccines.

The coronavirus disease 2019 (COVID-19) pandemic has highlighted the importance of developing novel vaccines. An ideal vaccine should trigger an intense immune reaction without causing significant side effects. In this study we found that substitution of tryptophan located in the cores of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) protein structures with certain smaller amino acids resulted in variants with melting temperatures of 33-37 °C.

The revelation of high-yield amino acids and probiotic characteristics of an intestinal Lacticaseibacillus rhamnosus X9C17.

With the popularization of probiotic products, tapping high-quality probiotics is crucial. Therefore, in this study, Lacticaseibacillus rhamnosus (X9C17) was selected from a pool of 54 isolates for its remarkable ability to survive in simulated gastrointestinal fluid (Gastric juice: 81.08 %, intestinal juice: 48 %), after which its probiotic characteristics were evaluated.

Ampicillin-derived carbon dots as the sensitive probe for the detection of Fe and Cu in living cells and water samples.

Water-soluble N-doped fluorescent (FL) carbon dots (ACDs) were successfully fabricated hydrothermally using ampicillin sodium as sole precursor. The produced ACDs exhibit satisfactory optical behavior, favorable photostability, and acceptable water solubility. With bright blue emission at 450 nm, the ACDs were utilized for multivariate sensing Fe and Cu based on the synergistic effect of the inner filter effect (IFE) and static quenching with detection limits of 0.

Molecular mechanism of proteolytic cleavage-dependent activation of CadC-mediated response to acid in E. coli.

Colonizing in the gastrointestinal tract, Escherichia coli confronts diverse acidic challenges and evolves intricate acid resistance strategies for its survival. The lysine-mediated decarboxylation (Cad) system, featuring lysine decarboxylase CadA, lysine/cadaverine antiporter CadB, and transcriptional activator CadC, plays a crucial role in E. coli's adaptation to moderate acidic stress.

The cAMP receptor protein from Gardnerella vaginalis is not regulated by ligands.

Overgrowth of Gardnerella vaginalis causes an imbalance in vaginal microecology. The pathogenicity of G. vaginalis is directly regulated by the cAMP receptor protein (CRP).

Development of an indirect ELISA for detecting Toxoplasma gondii IgG antibodies based on a recombinant TgIMP1 protein.

Toxoplasma gondii (T. gondii) is widely spread around the world, which can cause serious harm to immunosuppressed patients. Currently, the commercial test kits are poor at assessing T.

Proteomics analysis of Toxoplasma gondii merozoites reveals regulatory proteins involved in sexual reproduction.

Sexual reproduction plays a crucial role in the transmission and life cycle of toxoplasmosis. The merozoites are the only developmental stage capable of differentiation into male and female gametes, thereby initiating sexual reproduction to form oocysts that are excreted into the environment. Hence, our study aimed to perform proteomic analyses of T.

Label-free quantitative proteomic analyses of mouse astrocytes provides insight into the host response mechanism at different developmental stages of Toxoplasma gondii.

Toxoplasma gondii (T. gondii) is an opportunistic parasite that can infect the central nervous system (CNS), causing severe toxoplasmosis and behavioral cognitive impairment. Mortality is high in immunocompromised individuals with toxoplasmosis, most commonly due to reactivation of infection in the CNS.

RT-IVT method allows multiplex real-time quantification of in vitro transcriptional mRNA production.

For the past 30 years, in vitro transcription (IVT) technology has been extensively used for RNA production or for basic transcriptional mechanism research. However, methods for mRNA quantification still need to be improved. In this study, we designed a RT-IVT method using binary fluorescence quencher (BFQ) probes and the PBCV-1 DNA ligase to quantify mRNA production in real-time by fluorescence resonance energy transfer (FRET) and RNA-splinted DNA ligation.

Transcriptomic and Proteomic Analysis of Responding to Acidic pH and Hydrogen Peroxide Stress.

is the main pathogen that causes bacterial vaginosis. In the healthy vaginal microecological environment of a woman, the lactobacilli produce lactate and hydrogen peroxide to inhibit the growth of pathogens such as . The lack of lactobacilli results in a high pH and low hydrogen peroxide in the vagina which facilitate growth, leading to the imbalance of the vaginal microecology.

A fast RT-qPCR system significantly shortens the time for SARS-CoV-2 nucleic acid test.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a serious threat to global development. Rapid and accurate diagnosis is critical for containing the pandemic and treating patients in time. As the gold standard for SARS-CoV-2 diagnosis, the qualitative reverse transcription-PCR (RT-qPCR) test has long been criticized for its long detection time.

SARS-CoV-2 RdRp uses NDPs as a substrate and is able to incorporate NHC into RNA from diphosphate form molnupiravir.

The coronavirus disease 2019 has been ravaging throughout the world for three years and has severely impaired both human health and the economy. The causative agent, severe acute respiratory syndrome coronavirus 2 employs the viral RNA dependent RNA polymerase (RdRp) complex for genome replication and transcription, making RdRp an appealing target for antiviral drug development. Systematic characterization of RdRp will undoubtedly aid in the development of antiviral drugs targeting RdRp.

Antibiotic resistance and pathogenicity assessment of various sp. strains in local China.

overgrowth is the primary cause of bacterial vaginosis (BV), a common vaginal infection with incidences as high as 23-29% worldwide. Here, we studied the pathogenicity, drug resistance, and prevalence of varying spp. We isolated 20 strains from vaginal samples of 31 women in local China.

Structure-Based Primer Design Minimizes the Risk of PCR Failure Caused by SARS-CoV-2 Mutations.

The coronavirus disease 2019 (COVID-19) has caused and is still causing tremendous damage to the global economy and human health. Qualitative reverse transcription-PCR (RT-qPCR) is the golden standard for COVID-19 test. However, the SARS-CoV-2 variants may not only make vaccine less effective but also evade RT-qPCR test.

Analysis of COVID-19 Name Varieties in Chinese Mass and Social Media.

The sudden appearance of a new epidemic disease in China created the need for names identifying that disease. Between December 2019 and January 2020, a variety of severe pneumonia-related disease names suddenly appeared, and more name varieties kept coming up afterwards. To better understand the introduction and spread of these names, 16 different COVID-19-related name varieties were selected covering the period from the end of December 2019, when the epidemic started, to mid-March 2020, a moment at which the term competition had stabilized.

If the Coronavirus Doesn't Scare You, the Banners Will-A Case Study of Early COVID-19 Banners.

As a crucial element of China's political and cultural life, "banners," or biāoyǔ, have been around for decades, in support of national-level policies such as family planning and the governing mottos of Presidents. The banners that have emerged during the Covid-19 pandemic which was also the subject of a national-level driven policy, have been involved in a nation-wide public debate over the language styles of banners used to urge people to stay indoors. Based on the analysis of the early COVID-19 banners and the related online comments, this article analyzes the language style patterns of the banners and the mode of banner circulation.

High-Quality Genome Resource of the Pathogen of () Causing Kiwifruit Soft Rot.

spp. are critical plant pathogens that cause wood cankers, wilt, dieback, and fruit rot in a wide variety of economic plant hosts and are regarded as one of the most acute threats faced by the kiwifruit industry worldwide. NJD1 is a highly pathogenic isolate of soft rot of kiwifruit.

The YdiU Domain Modulates Bacterial Stress Signaling through Mn-Dependent UMPylation.

Sensing stressful conditions and adjusting the cellular metabolism to adapt to the environment are essential activities for bacteria to survive in variable situations. Here, we describe a stress-related protein, YdiU, and characterize YdiU as an enzyme that catalyzes the covalent attachment of uridine-5'-monophosphate to a protein tyrosine/histidine residue, an unusual modification defined as UMPylation. Mn serves as an essential co-factor for YdiU-mediated UMPylation.

YdiV regulates Escherichia coli ferric uptake by manipulating the DNA-binding ability of Fur in a SlyD-dependent manner.

Iron is essential for all bacteria. In most bacteria, intracellular iron homeostasis is tightly regulated by the ferric uptake regulator Fur. However, how Fur activates the iron-uptake system during iron deficiency is not fully elucidated.