Engineering the cellular mechanical microenvironment to regulate stem cell chondrogenesis: Insights from a microgel model.

Biophysical cues (especially mechanical cues) embedded in cellular microenvironments show a critical impact on stem cell fate. Despite the capability of traditional hydrogels to mimic the feature of extracellular matrix (ECM) and tune their physicochemical properties via diverse approaches, their relatively large size not only induces biased results, but also hinders high-throughput screening and analysis. In this paper, a microgel model is proposed to recapitulate the role of 3D mechanical microenvironment on stem cell behaviors especially chondrogenesis in vitro.

Hierarchical patterning via dynamic sacrificial printing of stimuli-responsive hydrogels.

Inspired by stimuli-tailored dynamic processes that spatiotemporally create structural and functional diversity in biology, a new hierarchical patterning strategy is proposed to induce the emergence of complex multidimensional structures via dynamic sacrificial printing of stimuli-responsive hydrogels. Using thermally responsive gelatin (Gel) and pH-responsive chitosan (Chit) as proof-of-concept materials, we demonstrate that the initially printed sacrificial material (Gel/Chit-H hydrogel with a single gelatin network) can be converted dynamically into non-sacrificial material (Gel/Chit-H-Citr hydrogel with gelatin and an electrostatic citrate-chitosan dual network) under stimulus cues (citrate ions). Complex hierarchical structures and functions can be created by controlling either the printing patterns of citrate ink or the diffusion time of citrate ions into the Gel/Chit-H hydrogel.

Multifunctional Conductive Hydrogel/Thermochromic Elastomer Hybrid Fibers with a Core-Shell Segmental Configuration for Wearable Strain and Temperature Sensors.

Flexible wearable sensors are emerging as next-generation tools to collect information from the human body and surroundings in a smart, friendly, and real-time manner. A new class of such sensors with various functionality and amenability for the human body is essential for this goal. Unfortunately, the majority of the wearable sensors reported so far in the literature were of a single function (mostly strain sensors) and just a prototype without thinking of continuous mass production.