Molecular characterization, expression analysis of gene and its potential role in intramuscular adipocyte differentiation of goat.

is a cytokine secreted by adipose cells and plays an important regulatory role in adipocyte differentiation and lipid metabolism. However, the function of on intramuscular fat (IMF) deposition is still unknown in goat. Here, we cloned the cDNA of goat gene using quantitative real-time PCR (qRT-PCR) technique and further clarified the expression pattern of in various tissues of goat.

Cloning and Expression of SFRP5 in Tibetan Chicken and its Relationship with IMF Deposition.

Secreted frizzled related protein 5 (SFRP5), an anti-inflammatory adipokine, is relevant to the adipocyte differentiation. In order to clarify its role in regulating intramuscular fat (IMF) deposition in Tibetan chicken, the full-length sequence of the Tibetan chicken SFRP5 gene was cloned. The relative expression of SFRP5 gene was detected using quantitative RT-PCR in various tissues of 154 days old Tibetan chicken, as well as in breast muscle, thigh muscle, and adipose tissue at different growth stages.

Selection of reference genes for gene expression studies related to intramuscular fat deposition in Capra hircus skeletal muscle.

The identification of suitable reference genes is critical for obtaining reliable results from gene expression studies using quantitative real-time PCR (qPCR) because the expression of reference genes may vary considerably under different experimental conditions. In most cases, however, commonly used reference genes are employed in data normalization without proper validation, which may lead to incorrect data interpretation. Here, we aim to select a set of optimal reference genes for the accurate normalization of gene expression associated with intramuscular fat (IMF) deposition during development.

Molecular cloning, expression and purification of lactoferrin from Tibetan sheep mammary gland using a yeast expression system.

This paper reports the successful expression of a lactoferrin gene-obtained from the mammary gland tissue of Tibetan sheep-in the yeast Pichia pastoris GS115 using pPICZαA as the recombinant plasmid and α-factor signal sequence for secretion. The recombinant lactoferrin was purified by ammonium sulfate precipitation, ion-exchange column chromatography and gel-filtration chromatography, and it had a molecular mass of 76kDa. We obtained an expression yield of >60mgL(-1) and specific activity of 2533.