p16 and p53 can Serve as Prognostic Markers for Head and Neck Squamous Cell Carcinoma.

Objective: The present study aimed to explore the expression and clinical significance of human papilloma virus-related pathogenic factors (p16, cyclin D1, p53) in patients with head and neck squamous cell carcinoma (HNSCC) and construct a predictive model.

Methods: The Cancer Genome Atlas was used to obtain clinical data for 112 patients with HNSCC. Expression of p16, p53, and cyclin D1 was quantified.

A case of oral tuberculous ulcer and literature review.

Our purpose is to clearly diagnose the tongue and back tuberculosis ulcer through detailed medical history collection combined with examination, so as to provide certain experience for the diagnosis and treatment of oral tuberculosis.

[Effects of miR-31-5p on HIF-1α/BNIP3 signaling pathway and the expression of osteoblast-related factors of dental pulp stem cells].

Purpose: To investigate the effects of microRNA-31-5p (miR-31-5p) on the signal pathway of hypoxia inducible factor-1α (HIF-1α)/Bcl-2/adenovirus E1B 19-kDa-interacting protein 3(BNIP3) and the expression of osteoblast-related factors of dental pulp stem cells(DPSCs).

Methods: Human dental pulp stem cells (DPSCs) were cultured in vitro and divided into the control group (no transfection), mimic NC group (transfected with negative control-miR-31-5p), miR-31-5p mimic group (transfected with hsa-miR-31-5p mimic), siRNA NC group (transfected with nonsense siRNA) and miR-31-5p siRNA group (transfected with miR-31-5p siRNA).The expressions of miR-31-5p, HIF-1α, BNIP3, alkaline phosphatase(ALP) and Runt-related transcription factor-2(Runx2) mRNA in DPSCs were detected by real-time fluorescence quantitative PCR; the proliferation of DPSCs was detected by MTT; ALP activity of DPSCs was detected by ALP activity test kit; and the protein expressions of HIF-1α, BNIP3 and Runx2 in DPSCs were detected by Western blot.

Identification of autophagy-related biomarker and analysis of immune infiltrates in oral carcinoma.

Background: Autophagy plays a vital role in the progression of the tumor. We aimed to investigate the expression, prognostic value, and immune infiltration of autophagy-related genes in oral carcinoma via bioinformatics analysis.

Methods: The microarray datasets (GSE146483 and GSE23558) of oral carcinoma were downloaded from Gene Expression Omnibus (GEO) database.

BCCIPβ facilitates p53 ubiquitination via binding with E6 protein in high-risk HPV positive head and neck squamous cell carcinoma.

BRCA2 And CDKN1A Interacting Protein (BCCIP) is initially identified as a tumor suppressor. Some recent studies confirmed its p53 binding capability. In this study, we explored the regulatory effect of BCCIPβ on p53 stability in HPV-positive and HPV-negative HNSCC cells.

-targeted RNA interference inhibits the proliferation of human oral squamous carcinoma HN12 cells.

In the present study, RNA interference (RNAi) was used to investigate the effect of vascular cell adhesion molecule 1 () silencing on the proliferation of human oral squamous carcinoma HN12 cells. HN12 cells were divided into three groups: The untreated blank control cell group (CK), the negative control group transfected with non-homologous vector (NC) and the positive group transfected with the target sequence small hairpin RNA (KD). Reverse-transcription polymerase chain reaction and western blot analysis were used to examine the effects of -knockdown on the mRNA expression of and subsequent protein expression.

[Dental pulp stem cells modified by HIF-1α can differentiate into blood vessels].

Purpose: To investigate the method of differentiating dental pulp stem cells (DPSCs) modified by HIF-1α into blood vessels.

Methods: DPSCs were extracted from teeth samples from 20 patients and were identified by Strol-1 and CD146. DPSCs were divided into experimental group and control group according to DPSCs were modified by HIF-1α not or.

Association of Decreased Expression of Serum miR-9 with Poor Prognosis of Oral Squamous Cell Carcinoma Patients.

Background: Dysregulation of miR-9 is a common feature of many types of cancers, including oral squamous cell carcinoma (OSCC). However, whether the expression level of serum miR-9 is changed in patients with OSCC remains unknown.

Material/methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to examine the expression level of serum miR-9 in OSCC patients, oral leukoplakia (OLK) patients, and healthy volunteers, then we evaluated the association between serum miR-9 expression level and clinical outcome of OSCC patients.

Infantile fibromatosis of the pterygomandibular space.

Infantile fibromatosis (IF) is a benign, nonmetastasizing but locally aggressive tumor. Pterygomandibular space is deep and obscure, and IF in this space is seldom reported. We describe a typical case of IF in the pterygomandibular space that occurred in a 3-year-old girl.

Intraosseous mucoepidermoid carcinoma of jaws: report of 24 cases.

Objective: Intraosseous mucoepidermoid carcinoma of jaws is rare, and management of the disease remains poorly understood. The aim of this study was to assist the diagnosis and treatment of the tumor.

Study Design: The records of clinical data and follow-up information were collected from 1996 to 2010 and retrospectively analyzed for clinical features, surgical intervention, and prognosis.

Clinical management of pediatric aggressive fibromatosis involving the mandible.

Background: Pediatric aggressive fibromatosis (AF) is a rare, benign tumor with locally infiltrative growth. Therefore, how to prevent reoccurrence while maintaining the mandible contour and continuity as much as possible is very important when the mandible is involved.

Procedure: We selected 10 pediatric patients with AF involving the mandible in our department between January 2001 and December 2011.

Pleomorphic adenoma of the salivary glands in children and adolescents.

Background: Pleomorphic adenomas (PAs) of the salivary glands are rare in children and adolescents. We reviewed the clinical manifestations, pathologic features, treatment, and prognosis of salivary gland PA in 90 children and adolescents.

Methods: Clinical data including age, sex, location, symptoms, the period of evolution of the symptoms, pathologic type, and surgical treatment were evaluated.

Blood vessel formation in the tissue-engineered bone with the constitutively active form of HIF-1α mediated BMSCs.

The successful clinical outcome of the implanted tissue-engineered bone is dependent on the establishment of a functional vascular network. A gene-enhanced tissue engineering represents a promising approach for vascularization. Our previous study indicated that hypoxia-inducible factor-1α (HIF-1α) can up-regulate the expression of vascular endothelial growth factor (VEGF) and stromal-derived factor 1 (SDF-1) in bone mesenchymal stem cells (BMSCs).

Clinical management of peripheral ameloblastoma.

Peripheral ameloblastoma is a rare epithelial odontogenic tumor, limited to the soft tissues of the gingiva or oral mucosa. Peripheral ameloblastoma represents approximately 2% to 10% of all ameloblastomas. It is always considered to be benign, but occasionally it may be locally aggressive or with malignant potential.

Clinical management of cervical ectopic thymus in children.

Objective: Cervical ectopic thymus (CET) is an extremely uncommon etiology of a neck mass in an infant. The aim of this study was to study and analyze the clinical manifestations, management principles, and pathological diagnosis of CET.

Methods: From 1995 to 2010, a total of 25,237 cases of head and neck lesions were treated in the Department of Oral and Maxillofacial Surgery, Shanghai Ninth People's Hospital.

Solitary neurofibroma arising from the infratemporal fossa in a child.

Neurofibromas are derived from the nerve sheath and are commonly located in the head and neck region. They usually occur between the ages of 30 and 50 years. Neurofibromas arising from the infratemporal fossa are quite rare, especially in children.