Characterization of Extended-Spectrum β-Lactamase-Producing in Animal Farms in Hunan Province, China.

Multi-drug resistance of bacteria producing extended-spectrum β-lactamase (ESBL) is a public health challenge. Thus, this study aimed to investigate the antimicrobial susceptibility of ESBL-producing (ESBL-EC) in Hunan Province, China. A total of 1366 fecal samples were collected from pig, chicken, and cattle farms over a six-year period, which were assessed using strain isolation, 16S rRNA identification, polymerase chain reaction, drug sensitivity testing, whole-genome sequencing, and bioinformatics analysis.

Serotyping and Antimicrobial Resistance Profiling of Multidrug-Resistant Non-Typhoidal from Farm Animals in Hunan, China.

Non-typhoidal (NTS) is a foodborne pathogen and a prevalent causative agent for disease outbreaks globally. The enterica serovar 4,[5],12:i:- (S.4,[5],12:i:-) belongs to the monophasic variant of , which is of current global concern.

Characteristics of (X4)-Producing in Chicken and Pig Farms in Hunan Province, China.

Background: The plasmid-mediated tigecycline resistance gene (X4) confers a high level of resistance to tigecycline. The experiment aims to investigate the prevalence and characterization of (X4) in isolates from chicken and pig farms in Hunan province, China.

Methods: A total of six (X4) positive strains were identified in 257 derived from chicken samples in Xiangtan city ( = 2), pig samples in Xiangxiang city ( = 1), Chenzhou city ( = 2), and Zhuzhou city ( = 1).

Characteristics of the plasmid-mediated colistin-resistance gene in isolated from a veterinary hospital in Shanghai.

The mobile colistin-resistance gene is primarily detected in species, such as and , and represents a significant public health threat. Herein, we investigated the prevalence and characteristics of -positive . (MCRPEC) in hospitalized companion animals in a pet hospital in Shanghai, China, from May 2021 to July 2021.

Occurrence of -Positive spp. in a Pig Farm of China.

Antibiotics have been extensively used to ensure the productivity of animals on intensive livestock farms. Accordingly, antimicrobial-resistant organisms, which can be transmitted to humans via the food chain, pose a threat to public health. The Enterobacterium antimicrobial resistance gene, , is a transmissible gene that has attracted widespread attention.

/ mutation might act as the predictor for chemotherapy response in lung adenocarcinoma and lung squamous carcinoma patients.

Background: Chemotherapy is the preferred treatment in many types of cancer including lung cancer. However, most of patients resist chemotherapy resulting in disease progressive and recurrence. Titin () mutation is proved as a beneficial role in lung squamous carcinoma (LUSC), but the predictive role on chemotherapy resistance of lung cancer is still limited and discussable.

Structure and function encoding of a bidirectional activatable synergetic DNA machine for speeded and ultrasensitive determination of microRNAs.

This work describes the unique design of a bidirectional activatable synergetic DNA machine (BAS-DNA machine) for speeded and ultrasensitive determination of microRNA-21 (miR-21), a well-known biomarker for biomedical research and early diagnosis of lung cancer. The BAS-DNA machine is composed by a pair of track strands (Track 1 and Track 2) encoding with two regions in the opposite direction for miR-21 recognition. Introduction of miR-21 can hybridize either with Track 1 or with Track 2 to activate the BAS-DNA machine with a synergistic effect for speeded amplifying the fluorescence signal.

Clinical characteristics of 78 cases of patients infected with coronavirus disease 2019 in Wuhan, China.

Coronavirus disease 2019 (COVID-19) emerged in Wuhan and rapidly spread throughout the world in December 2019. The present study aimed to describe the clinical characteristics and laboratory findings of 78 patients with COVID-19 in order to enhance the understanding of the disease. Medical records and data of 78 patients with COVID-19, including demographics, clinical features, laboratory findings and radiological characteristics, were collected and analyzed.

miR-218 expression in osteosarcoma tissues and its effect on cell growth in osteosarcoma cells.

Objective: To investigate the expression of miR-218 and its clinical significance in osteosarcoma tissues and explore its effect on proliferation and apoptosis in osteosarcoma cells.

Methods: miR-218 expression was detected in 76 samples of surgically resected osteosarcoma and matched normal tumor-adjacent tissues using quantitative reverse transcription polymerase chain reaction (qRT-PCR). MiR-218 was over-expressed by exogenous miR-218 plasmids in Saos-2 cells, and then BrdU cell proliferation assay and flow cytometry were used to determine cell proliferation and apoptosis.